Antioxidant profiling of ginger via reaction flow chromatography

Autores
Zhou, Xian; Power, Declan; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; Shalliker, R. Andrew; Li, Chunguang; Soliven, Arianne
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective detection, in particular the antioxidant profiling of natural products. This study employed RF for the first time to identify antioxidants in a commercial ginger sample. This demonstrated the previously validated assay's ease and power to extract information about the natural product's antioxidant properties. Due to the simplicity involved with data analysis and peak matching process, the following information was revealed between the chemical and antioxidant profiles: three of the strongest antioxidant activity peaks in the ginger sample (593 nm) did not correlate with the three most abundant chemical profile peaks (UV absorbance at 254 and 280 nm); the ratio of seven antioxidant peaks may be potentially used for food authenticity purposes, and future research should target these peaks for the early discovery of novel antioxidants sourced in ginger. Utilization of this previously validated assay provided the resolution of numerous peaks in the ginger extract and information associated with their antioxidant attributes and chemical abundance. This approach is more informative than total antioxidant assays that lack compound specificity information. Furthermore, it is superior to mass spectrometric (MS) assays that cannot evaluate each compound's antioxidant strength, and does not involve the expense involved in the acquisition and maintenance of the MS detection hardware, and does not require the high level of expertise needed to conduct the MS data analysis.
Fil: Zhou, Xian. University of Western Sydney; Australia
Fil: Power, Declan. University of Western Sydney; Australia
Fil: Jones, Andrew. University of Western Sydney; Australia
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Dennis, Gary R.. University of Western Sydney; Australia
Fil: Shalliker, R. Andrew. University of Western Sydney; Australia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Fil: Li, Chunguang. University of Western Sydney; Australia
Fil: Soliven, Arianne. University of Western Sydney; Australia
Materia
ANTIOXIDANT
FERRIC REDUCING ANTIOXIDANT POWER ASSAY
GINGER
POST COLUMN DERIVATIZATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159862
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/159862
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant profiling of ginger via reaction flow chromatographyZhou, XianPower, DeclanJones, AndrewAcquaviva, AgustínDennis, Gary R.Shalliker, R. AndrewLi, ChunguangSoliven, ArianneANTIOXIDANTFERRIC REDUCING ANTIOXIDANT POWER ASSAYGINGERPOST COLUMN DERIVATIZATIONREACTION FLOW CHROMATOGRAPHYSELECTIVE DETECTIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective detection, in particular the antioxidant profiling of natural products. This study employed RF for the first time to identify antioxidants in a commercial ginger sample. This demonstrated the previously validated assay's ease and power to extract information about the natural product's antioxidant properties. Due to the simplicity involved with data analysis and peak matching process, the following information was revealed between the chemical and antioxidant profiles: three of the strongest antioxidant activity peaks in the ginger sample (593 nm) did not correlate with the three most abundant chemical profile peaks (UV absorbance at 254 and 280 nm); the ratio of seven antioxidant peaks may be potentially used for food authenticity purposes, and future research should target these peaks for the early discovery of novel antioxidants sourced in ginger. Utilization of this previously validated assay provided the resolution of numerous peaks in the ginger extract and information associated with their antioxidant attributes and chemical abundance. This approach is more informative than total antioxidant assays that lack compound specificity information. Furthermore, it is superior to mass spectrometric (MS) assays that cannot evaluate each compound's antioxidant strength, and does not involve the expense involved in the acquisition and maintenance of the MS detection hardware, and does not require the high level of expertise needed to conduct the MS data analysis.Fil: Zhou, Xian. University of Western Sydney; AustraliaFil: Power, Declan. University of Western Sydney; AustraliaFil: Jones, Andrew. University of Western Sydney; AustraliaFil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Dennis, Gary R.. University of Western Sydney; AustraliaFil: Shalliker, R. Andrew. University of Western Sydney; Australia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; ArgentinaFil: Li, Chunguang. University of Western Sydney; AustraliaFil: Soliven, Arianne. University of Western Sydney; AustraliaNatural Products2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159862Zhou, Xian; Power, Declan; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; et al.; Antioxidant profiling of ginger via reaction flow chromatography; Natural Products; Natural Product Communications; 16; 9; 7-2021; 1-61934-578XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/1934578X211035286info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1934578X211035286info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:56Zoai:ri.conicet.gov.ar:11336/159862instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:56.636CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant profiling of ginger via reaction flow chromatography
title Antioxidant profiling of ginger via reaction flow chromatography
spellingShingle Antioxidant profiling of ginger via reaction flow chromatography
Zhou, Xian
ANTIOXIDANT
FERRIC REDUCING ANTIOXIDANT POWER ASSAY
GINGER
POST COLUMN DERIVATIZATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
title_short Antioxidant profiling of ginger via reaction flow chromatography
title_full Antioxidant profiling of ginger via reaction flow chromatography
title_fullStr Antioxidant profiling of ginger via reaction flow chromatography
title_full_unstemmed Antioxidant profiling of ginger via reaction flow chromatography
title_sort Antioxidant profiling of ginger via reaction flow chromatography
dc.creator.none.fl_str_mv Zhou, Xian
Power, Declan
Jones, Andrew
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Li, Chunguang
Soliven, Arianne
author Zhou, Xian
author_facet Zhou, Xian
Power, Declan
Jones, Andrew
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Li, Chunguang
Soliven, Arianne
author_role author
author2 Power, Declan
Jones, Andrew
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Li, Chunguang
Soliven, Arianne
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT
FERRIC REDUCING ANTIOXIDANT POWER ASSAY
GINGER
POST COLUMN DERIVATIZATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
topic ANTIOXIDANT
FERRIC REDUCING ANTIOXIDANT POWER ASSAY
GINGER
POST COLUMN DERIVATIZATION
REACTION FLOW CHROMATOGRAPHY
SELECTIVE DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective detection, in particular the antioxidant profiling of natural products. This study employed RF for the first time to identify antioxidants in a commercial ginger sample. This demonstrated the previously validated assay's ease and power to extract information about the natural product's antioxidant properties. Due to the simplicity involved with data analysis and peak matching process, the following information was revealed between the chemical and antioxidant profiles: three of the strongest antioxidant activity peaks in the ginger sample (593 nm) did not correlate with the three most abundant chemical profile peaks (UV absorbance at 254 and 280 nm); the ratio of seven antioxidant peaks may be potentially used for food authenticity purposes, and future research should target these peaks for the early discovery of novel antioxidants sourced in ginger. Utilization of this previously validated assay provided the resolution of numerous peaks in the ginger extract and information associated with their antioxidant attributes and chemical abundance. This approach is more informative than total antioxidant assays that lack compound specificity information. Furthermore, it is superior to mass spectrometric (MS) assays that cannot evaluate each compound's antioxidant strength, and does not involve the expense involved in the acquisition and maintenance of the MS detection hardware, and does not require the high level of expertise needed to conduct the MS data analysis.
Fil: Zhou, Xian. University of Western Sydney; Australia
Fil: Power, Declan. University of Western Sydney; Australia
Fil: Jones, Andrew. University of Western Sydney; Australia
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Grupo Cromatografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Dennis, Gary R.. University of Western Sydney; Australia
Fil: Shalliker, R. Andrew. University of Western Sydney; Australia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Fil: Li, Chunguang. University of Western Sydney; Australia
Fil: Soliven, Arianne. University of Western Sydney; Australia
description Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective detection, in particular the antioxidant profiling of natural products. This study employed RF for the first time to identify antioxidants in a commercial ginger sample. This demonstrated the previously validated assay's ease and power to extract information about the natural product's antioxidant properties. Due to the simplicity involved with data analysis and peak matching process, the following information was revealed between the chemical and antioxidant profiles: three of the strongest antioxidant activity peaks in the ginger sample (593 nm) did not correlate with the three most abundant chemical profile peaks (UV absorbance at 254 and 280 nm); the ratio of seven antioxidant peaks may be potentially used for food authenticity purposes, and future research should target these peaks for the early discovery of novel antioxidants sourced in ginger. Utilization of this previously validated assay provided the resolution of numerous peaks in the ginger extract and information associated with their antioxidant attributes and chemical abundance. This approach is more informative than total antioxidant assays that lack compound specificity information. Furthermore, it is superior to mass spectrometric (MS) assays that cannot evaluate each compound's antioxidant strength, and does not involve the expense involved in the acquisition and maintenance of the MS detection hardware, and does not require the high level of expertise needed to conduct the MS data analysis.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159862
Zhou, Xian; Power, Declan; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; et al.; Antioxidant profiling of ginger via reaction flow chromatography; Natural Products; Natural Product Communications; 16; 9; 7-2021; 1-6
1934-578X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159862
identifier_str_mv Zhou, Xian; Power, Declan; Jones, Andrew; Acquaviva, Agustín; Dennis, Gary R.; et al.; Antioxidant profiling of ginger via reaction flow chromatography; Natural Products; Natural Product Communications; 16; 9; 7-2021; 1-6
1934-578X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/1934578X211035286
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1934578X211035286
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natural Products
publisher.none.fl_str_mv Natural Products
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614222760116224
score 13.070432

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