Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina

Autores
Brusa, Victoria; Aliverti, Virginia; Aliverti, Florencia; Ortega, Emanuel; Torre, Julián Horacio de la; Linares, Luciano Héctor; Sanz, Marcelo E.; Etcheverría, Analía I.; Padola, Nora Lía; Galli, Lucía; Peral García, Pilar; Copes, Julio Alberto; Leotta, Gerardo Aníbal
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, meat mincing machine, and manipulator hands (n = 450) obtained from 90 retail markets over a nine-month period. The STEC isolates were serotyped and virulence genes as stx (Shiga toxin), rfbO157] (O157 lipopolysaccharide), fliCH7 (H7 flagellin), eae (intimin), ehxA (enterohemolysin) and saa (STEC autoagglutinating adhesin), were determined. STEC O157 were identified in 23 (25.5%) beef samples and 16 (4.4%) environmental samples, while STEC non-O157 were present in 47 (52.2%) and 182 (50.5%), respectively. Among 54 strains isolated, 17 were STEC O157:H7 and 37 were STEC non-O157. The prevalent genotype for O157 was stx2/eae/ehxA/fliCH7 (83.4%), and for STEC non-O157 the most frequent ones were stx1/stx2/saa/ehxA (29.7%); stx2 (29.7%); and stx2/saa/ehxA (27%). None of the STEC non-O157 strains were eae-positive. Besides O157:H7, other 20 different serotypes were identified, being O8:H19, O178:H19, and O174:H28 the prevalent. Strains belonging to the same serotype could be isolated from different sources of the same retail market. Also, the same serotype could be detected in different stores. In conclusion, screening techniques are increasingly sensitive, but the isolation of STEC non-O157 is still a challenge. Moreover, with the results obtained from the present work, although more studies are needed, cross-contamination between meat and the environment could be suspected.
Instituto de Genética Veterinaria
Materia
Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85414

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/85414
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from ArgentinaBrusa, VictoriaAliverti, VirginiaAliverti, FlorenciaOrtega, EmanuelTorre, Julián Horacio de laLinares, Luciano HéctorSanz, Marcelo E.Etcheverría, Analía I.Padola, Nora LíaGalli, LucíaPeral García, PilarCopes, Julio AlbertoLeotta, Gerardo AníbalCiencias VeterinariasArgentinaEnvironmental samplesRaw ground beefRetail marketsStecShiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, meat mincing machine, and manipulator hands (n = 450) obtained from 90 retail markets over a nine-month period. The STEC isolates were serotyped and virulence genes as stx (Shiga toxin), rfbO157] (O157 lipopolysaccharide), fliCH7 (H7 flagellin), eae (intimin), ehxA (enterohemolysin) and saa (STEC autoagglutinating adhesin), were determined. STEC O157 were identified in 23 (25.5%) beef samples and 16 (4.4%) environmental samples, while STEC non-O157 were present in 47 (52.2%) and 182 (50.5%), respectively. Among 54 strains isolated, 17 were STEC O157:H7 and 37 were STEC non-O157. The prevalent genotype for O157 was stx2/eae/ehxA/fliCH7 (83.4%), and for STEC non-O157 the most frequent ones were stx1/stx2/saa/ehxA (29.7%); stx2 (29.7%); and stx2/saa/ehxA (27%). None of the STEC non-O157 strains were eae-positive. Besides O157:H7, other 20 different serotypes were identified, being O8:H19, O178:H19, and O174:H28 the prevalent. Strains belonging to the same serotype could be isolated from different sources of the same retail market. Also, the same serotype could be detected in different stores. In conclusion, screening techniques are increasingly sensitive, but the isolation of STEC non-O157 is still a challenge. Moreover, with the results obtained from the present work, although more studies are needed, cross-contamination between meat and the environment could be suspected.Instituto de Genética Veterinaria2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85414enginfo:eu-repo/semantics/altIdentifier/issn/2235-2988info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2012.00171info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:34Zoai:sedici.unlp.edu.ar:10915/85414Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:34.882SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
spellingShingle Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
Brusa, Victoria
Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
title_short Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_full Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_fullStr Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_full_unstemmed Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
title_sort Shiga toxin-producing <i>Escherichia coli</i> in beef retail markets from Argentina
dc.creator.none.fl_str_mv Brusa, Victoria
Aliverti, Virginia
Aliverti, Florencia
Ortega, Emanuel
Torre, Julián Horacio de la
Linares, Luciano Héctor
Sanz, Marcelo E.
Etcheverría, Analía I.
Padola, Nora Lía
Galli, Lucía
Peral García, Pilar
Copes, Julio Alberto
Leotta, Gerardo Aníbal
author Brusa, Victoria
author_facet Brusa, Victoria
Aliverti, Virginia
Aliverti, Florencia
Ortega, Emanuel
Torre, Julián Horacio de la
Linares, Luciano Héctor
Sanz, Marcelo E.
Etcheverría, Analía I.
Padola, Nora Lía
Galli, Lucía
Peral García, Pilar
Copes, Julio Alberto
Leotta, Gerardo Aníbal
author_role author
author2 Aliverti, Virginia
Aliverti, Florencia
Ortega, Emanuel
Torre, Julián Horacio de la
Linares, Luciano Héctor
Sanz, Marcelo E.
Etcheverría, Analía I.
Padola, Nora Lía
Galli, Lucía
Peral García, Pilar
Copes, Julio Alberto
Leotta, Gerardo Aníbal
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
topic Ciencias Veterinarias
Argentina
Environmental samples
Raw ground beef
Retail markets
Stec
dc.description.none.fl_txt_mv Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, meat mincing machine, and manipulator hands (n = 450) obtained from 90 retail markets over a nine-month period. The STEC isolates were serotyped and virulence genes as stx (Shiga toxin), rfbO157] (O157 lipopolysaccharide), fliCH7 (H7 flagellin), eae (intimin), ehxA (enterohemolysin) and saa (STEC autoagglutinating adhesin), were determined. STEC O157 were identified in 23 (25.5%) beef samples and 16 (4.4%) environmental samples, while STEC non-O157 were present in 47 (52.2%) and 182 (50.5%), respectively. Among 54 strains isolated, 17 were STEC O157:H7 and 37 were STEC non-O157. The prevalent genotype for O157 was stx2/eae/ehxA/fliCH7 (83.4%), and for STEC non-O157 the most frequent ones were stx1/stx2/saa/ehxA (29.7%); stx2 (29.7%); and stx2/saa/ehxA (27%). None of the STEC non-O157 strains were eae-positive. Besides O157:H7, other 20 different serotypes were identified, being O8:H19, O178:H19, and O174:H28 the prevalent. Strains belonging to the same serotype could be isolated from different sources of the same retail market. Also, the same serotype could be detected in different stores. In conclusion, screening techniques are increasingly sensitive, but the isolation of STEC non-O157 is still a challenge. Moreover, with the results obtained from the present work, although more studies are needed, cross-contamination between meat and the environment could be suspected.
Instituto de Genética Veterinaria
description Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, meat mincing machine, and manipulator hands (n = 450) obtained from 90 retail markets over a nine-month period. The STEC isolates were serotyped and virulence genes as stx (Shiga toxin), rfbO157] (O157 lipopolysaccharide), fliCH7 (H7 flagellin), eae (intimin), ehxA (enterohemolysin) and saa (STEC autoagglutinating adhesin), were determined. STEC O157 were identified in 23 (25.5%) beef samples and 16 (4.4%) environmental samples, while STEC non-O157 were present in 47 (52.2%) and 182 (50.5%), respectively. Among 54 strains isolated, 17 were STEC O157:H7 and 37 were STEC non-O157. The prevalent genotype for O157 was stx2/eae/ehxA/fliCH7 (83.4%), and for STEC non-O157 the most frequent ones were stx1/stx2/saa/ehxA (29.7%); stx2 (29.7%); and stx2/saa/ehxA (27%). None of the STEC non-O157 strains were eae-positive. Besides O157:H7, other 20 different serotypes were identified, being O8:H19, O178:H19, and O174:H28 the prevalent. Strains belonging to the same serotype could be isolated from different sources of the same retail market. Also, the same serotype could be detected in different stores. In conclusion, screening techniques are increasingly sensitive, but the isolation of STEC non-O157 is still a challenge. Moreover, with the results obtained from the present work, although more studies are needed, cross-contamination between meat and the environment could be suspected.
publishDate 2013
dc.date.none.fl_str_mv 2013
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info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2012.00171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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