Restriction site patterns in the ribosomal DNA of Camelidae

Autores
Semorile, Liliana Carmen; Crisci, Jorge Víctor; Arbeletche de Vidal Rioja, Lidia Beatriz
Año de publicación
1994
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI, BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17 restriction sites, six of which were mapped conserved in all the species. The other eleven corresponded to spacer regions and revealed variations between these taxa. The study showed that the two groups differ in the length of the internal transcribed spacer. Also they showed the existence of two regions of fast evolution on the opposite termini of the external spacer. A restriction site present at low frequency in the non-transcribed spacer of guanaco and llama was the only difference encountered within the South American group.
Instituto Multidisciplinario de Biología Celular
Materia
Biología
camelids
ribosomal DNA
restriction map
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/138433
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/138433
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Restriction site patterns in the ribosomal DNA of CamelidaeSemorile, Liliana CarmenCrisci, Jorge VíctorArbeletche de Vidal Rioja, Lidia BeatrizBiologíacamelidsribosomal DNArestriction mapThe restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI, BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17 restriction sites, six of which were mapped conserved in all the species. The other eleven corresponded to spacer regions and revealed variations between these taxa. The study showed that the two groups differ in the length of the internal transcribed spacer. Also they showed the existence of two regions of fast evolution on the opposite termini of the external spacer. A restriction site present at low frequency in the non-transcribed spacer of guanaco and llama was the only difference encountered within the South American group.Instituto Multidisciplinario de Biología Celular1994info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf115-122http://sedici.unlp.edu.ar/handle/10915/138433enginfo:eu-repo/semantics/altIdentifier/issn/0016-6707info:eu-repo/semantics/altIdentifier/issn/1573-6857info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00163760info:eu-repo/semantics/altIdentifier/pmid/7958935info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:12:52Zoai:sedici.unlp.edu.ar:10915/138433Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:12:52.667SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Restriction site patterns in the ribosomal DNA of Camelidae
title Restriction site patterns in the ribosomal DNA of Camelidae
spellingShingle Restriction site patterns in the ribosomal DNA of Camelidae
Semorile, Liliana Carmen
Biología
camelids
ribosomal DNA
restriction map
title_short Restriction site patterns in the ribosomal DNA of Camelidae
title_full Restriction site patterns in the ribosomal DNA of Camelidae
title_fullStr Restriction site patterns in the ribosomal DNA of Camelidae
title_full_unstemmed Restriction site patterns in the ribosomal DNA of Camelidae
title_sort Restriction site patterns in the ribosomal DNA of Camelidae
dc.creator.none.fl_str_mv Semorile, Liliana Carmen
Crisci, Jorge Víctor
Arbeletche de Vidal Rioja, Lidia Beatriz
author Semorile, Liliana Carmen
author_facet Semorile, Liliana Carmen
Crisci, Jorge Víctor
Arbeletche de Vidal Rioja, Lidia Beatriz
author_role author
author2 Crisci, Jorge Víctor
Arbeletche de Vidal Rioja, Lidia Beatriz
author2_role author
author
dc.subject.none.fl_str_mv Biología
camelids
ribosomal DNA
restriction map
topic Biología
camelids
ribosomal DNA
restriction map
dc.description.none.fl_txt_mv The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI, BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17 restriction sites, six of which were mapped conserved in all the species. The other eleven corresponded to spacer regions and revealed variations between these taxa. The study showed that the two groups differ in the length of the internal transcribed spacer. Also they showed the existence of two regions of fast evolution on the opposite termini of the external spacer. A restriction site present at low frequency in the non-transcribed spacer of guanaco and llama was the only difference encountered within the South American group.
Instituto Multidisciplinario de Biología Celular
description The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI, BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17 restriction sites, six of which were mapped conserved in all the species. The other eleven corresponded to spacer regions and revealed variations between these taxa. The study showed that the two groups differ in the length of the internal transcribed spacer. Also they showed the existence of two regions of fast evolution on the opposite termini of the external spacer. A restriction site present at low frequency in the non-transcribed spacer of guanaco and llama was the only difference encountered within the South American group.
publishDate 1994
dc.date.none.fl_str_mv 1994
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/138433
url http://sedici.unlp.edu.ar/handle/10915/138433
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0016-6707
info:eu-repo/semantics/altIdentifier/issn/1573-6857
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00163760
info:eu-repo/semantics/altIdentifier/pmid/7958935
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
115-122
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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