Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii

Autores
Suárez, Viviana Beatriz; Miriam Zago; Giraffa, Giorgio; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The bacteriophages Cb1/204 and Cb1/342 were obtained by induction from the commercial strain Lactobacillus delbrueckii subsp. lactis Cb1, and propagated on Lactobacillus delbrueckii subsp. lactis 204 (Lb.l 204) and Lactobacillusdelbrueckii subsp. bulgaricus 342 (Lb.b 342), respectively. By cross sensitivity, it was possible to detect a delay in the lysis of Lb.l 204 with Cb1/342 phage, while the adsorption rate was high (99.5%). Modified and unmodified phages were isolated using phage Cb1/342 and strain Lb.l 204. The EOP (Efficiency of Plaquing) values for the four phages (Cb1/204, Cb1/342, Cb1/342modified and Cb1/342unmodified) suggested that an R/M system modified the original temperate phage, and the BglII-DNA restriction patterns of these phages might point out the presence of a Type II R/M system. Also, the existence of also a Type I R/M system was demonstrated by PCR and nucleotide sequence, being the percentages of alignment homology with Type I R/M systems reported previously higher than 95 %. In this study it was possible to demonstrate that the native phage resistant mechanisms and the occurrence of prophages in commercial host strains, contribute strongly to diversify of the phage population in a factory environment.
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Miriam Zago. Consiglio Per la Ricerca In Agricoltura E L' Analisi del L' Economía Agraria. Centro de Ricerca Per Le Produzioni Foraggere E Lattier-casearie; Italia
Fil: Giraffa, Giorgio. Consiglio Per la Ricerca In Agricoltura E L' Analisi del L' Economía Agraria. Centro de Ricerca Per Le Produzioni Foraggere E Lattier-casearie; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
RESTRICTION / MODIFICATION SYSTEM
LYSOGENY
BACTERIOPHAGES
LACTOBACILLUS DELBRUECKII
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103391
Acceder
id CONICETDig_db437791c46186af6333b8acbad62ea3
oai_identifier_str oai:ri.conicet.gov.ar:11336/103391
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckiiSuárez, Viviana BeatrizMiriam ZagoGiraffa, GiorgioReinheimer, Jorge AlbertoQuiberoni, Andrea del LujanRESTRICTION / MODIFICATION SYSTEMLYSOGENYBACTERIOPHAGESLACTOBACILLUS DELBRUECKIIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The bacteriophages Cb1/204 and Cb1/342 were obtained by induction from the commercial strain Lactobacillus delbrueckii subsp. lactis Cb1, and propagated on Lactobacillus delbrueckii subsp. lactis 204 (Lb.l 204) and Lactobacillusdelbrueckii subsp. bulgaricus 342 (Lb.b 342), respectively. By cross sensitivity, it was possible to detect a delay in the lysis of Lb.l 204 with Cb1/342 phage, while the adsorption rate was high (99.5%). Modified and unmodified phages were isolated using phage Cb1/342 and strain Lb.l 204. The EOP (Efficiency of Plaquing) values for the four phages (Cb1/204, Cb1/342, Cb1/342modified and Cb1/342unmodified) suggested that an R/M system modified the original temperate phage, and the BglII-DNA restriction patterns of these phages might point out the presence of a Type II R/M system. Also, the existence of also a Type I R/M system was demonstrated by PCR and nucleotide sequence, being the percentages of alignment homology with Type I R/M systems reported previously higher than 95 %. In this study it was possible to demonstrate that the native phage resistant mechanisms and the occurrence of prophages in commercial host strains, contribute strongly to diversify of the phage population in a factory environment.Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Miriam Zago. Consiglio Per la Ricerca In Agricoltura E L' Analisi del L' Economía Agraria. Centro de Ricerca Per Le Produzioni Foraggere E Lattier-casearie; ItaliaFil: Giraffa, Giorgio. Consiglio Per la Ricerca In Agricoltura E L' Analisi del L' Economía Agraria. Centro de Ricerca Per Le Produzioni Foraggere E Lattier-casearie; ItaliaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaCambridge University Press2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103391Suárez, Viviana Beatriz; Miriam Zago; Giraffa, Giorgio; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii; Cambridge University Press; Journal of Dairy Research; 76; 12-2009; 433-4400022-0299CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029909990112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:32Zoai:ri.conicet.gov.ar:11336/103391instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:32.355CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
title Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
spellingShingle Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
Suárez, Viviana Beatriz
RESTRICTION / MODIFICATION SYSTEM
LYSOGENY
BACTERIOPHAGES
LACTOBACILLUS DELBRUECKII
title_short Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
title_full Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
title_fullStr Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
title_full_unstemmed Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
title_sort Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii
dc.creator.none.fl_str_mv Suárez, Viviana Beatriz
Miriam Zago
Giraffa, Giorgio
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author Suárez, Viviana Beatriz
author_facet Suárez, Viviana Beatriz
Miriam Zago
Giraffa, Giorgio
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author_role author
author2 Miriam Zago
Giraffa, Giorgio
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author2_role author
author
author
author
dc.subject.none.fl_str_mv RESTRICTION / MODIFICATION SYSTEM
LYSOGENY
BACTERIOPHAGES
LACTOBACILLUS DELBRUECKII
topic RESTRICTION / MODIFICATION SYSTEM
LYSOGENY
BACTERIOPHAGES
LACTOBACILLUS DELBRUECKII
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The bacteriophages Cb1/204 and Cb1/342 were obtained by induction from the commercial strain Lactobacillus delbrueckii subsp. lactis Cb1, and propagated on Lactobacillus delbrueckii subsp. lactis 204 (Lb.l 204) and Lactobacillusdelbrueckii subsp. bulgaricus 342 (Lb.b 342), respectively. By cross sensitivity, it was possible to detect a delay in the lysis of Lb.l 204 with Cb1/342 phage, while the adsorption rate was high (99.5%). Modified and unmodified phages were isolated using phage Cb1/342 and strain Lb.l 204. The EOP (Efficiency of Plaquing) values for the four phages (Cb1/204, Cb1/342, Cb1/342modified and Cb1/342unmodified) suggested that an R/M system modified the original temperate phage, and the BglII-DNA restriction patterns of these phages might point out the presence of a Type II R/M system. Also, the existence of also a Type I R/M system was demonstrated by PCR and nucleotide sequence, being the percentages of alignment homology with Type I R/M systems reported previously higher than 95 %. In this study it was possible to demonstrate that the native phage resistant mechanisms and the occurrence of prophages in commercial host strains, contribute strongly to diversify of the phage population in a factory environment.
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Miriam Zago. Consiglio Per la Ricerca In Agricoltura E L' Analisi del L' Economía Agraria. Centro de Ricerca Per Le Produzioni Foraggere E Lattier-casearie; Italia
Fil: Giraffa, Giorgio. Consiglio Per la Ricerca In Agricoltura E L' Analisi del L' Economía Agraria. Centro de Ricerca Per Le Produzioni Foraggere E Lattier-casearie; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description The bacteriophages Cb1/204 and Cb1/342 were obtained by induction from the commercial strain Lactobacillus delbrueckii subsp. lactis Cb1, and propagated on Lactobacillus delbrueckii subsp. lactis 204 (Lb.l 204) and Lactobacillusdelbrueckii subsp. bulgaricus 342 (Lb.b 342), respectively. By cross sensitivity, it was possible to detect a delay in the lysis of Lb.l 204 with Cb1/342 phage, while the adsorption rate was high (99.5%). Modified and unmodified phages were isolated using phage Cb1/342 and strain Lb.l 204. The EOP (Efficiency of Plaquing) values for the four phages (Cb1/204, Cb1/342, Cb1/342modified and Cb1/342unmodified) suggested that an R/M system modified the original temperate phage, and the BglII-DNA restriction patterns of these phages might point out the presence of a Type II R/M system. Also, the existence of also a Type I R/M system was demonstrated by PCR and nucleotide sequence, being the percentages of alignment homology with Type I R/M systems reported previously higher than 95 %. In this study it was possible to demonstrate that the native phage resistant mechanisms and the occurrence of prophages in commercial host strains, contribute strongly to diversify of the phage population in a factory environment.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103391
Suárez, Viviana Beatriz; Miriam Zago; Giraffa, Giorgio; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii; Cambridge University Press; Journal of Dairy Research; 76; 12-2009; 433-440
0022-0299
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103391
identifier_str_mv Suárez, Viviana Beatriz; Miriam Zago; Giraffa, Giorgio; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Evidence for the presence of restriction/modification systems in Lactobacillus delbrueckii; Cambridge University Press; Journal of Dairy Research; 76; 12-2009; 433-440
0022-0299
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029909990112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613697158250496
score 13.070432

Publicaciones similares