Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa

Autores
Castellani, Lucas Gabriel; Luchetti, Abril; Nilsson, Juliet Fernanda; Pérez Giménez, Julieta; Wegener, Caren; Schlüter, Andreas; Pühler, Alfred; Lagares, Antonio; Brom, Susana; Pistorio, Mariano; Niehaus, Karsten; Torres Tejerizo, Gonzalo Arturo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.
Instituto de Biotecnologia y Biologia Molecular
Materia
Biología
Botánica
Rhizobia
Symbiosis
Alfalfa
Exopolysaccharide
Nitrogen fixation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/124226

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spelling Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfaCastellani, Lucas GabrielLuchetti, AbrilNilsson, Juliet FernandaPérez Giménez, JulietaWegener, CarenSchlüter, AndreasPühler, AlfredLagares, AntonioBrom, SusanaPistorio, MarianoNiehaus, KarstenTorres Tejerizo, Gonzalo ArturoBiologíaBotánicaRhizobiaSymbiosisAlfalfaExopolysaccharideNitrogen fixationOne of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.Instituto de Biotecnologia y Biologia Molecular2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124226enginfo:eu-repo/semantics/altIdentifier/issn/1664-462Xinfo:eu-repo/semantics/altIdentifier/pmid/33643369info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.642576info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:48Zoai:sedici.unlp.edu.ar:10915/124226Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:48.381SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
title Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
spellingShingle Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
Castellani, Lucas Gabriel
Biología
Botánica
Rhizobia
Symbiosis
Alfalfa
Exopolysaccharide
Nitrogen fixation
title_short Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
title_full Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
title_fullStr Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
title_full_unstemmed Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
title_sort Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
dc.creator.none.fl_str_mv Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Wegener, Caren
Schlüter, Andreas
Pühler, Alfred
Lagares, Antonio
Brom, Susana
Pistorio, Mariano
Niehaus, Karsten
Torres Tejerizo, Gonzalo Arturo
author Castellani, Lucas Gabriel
author_facet Castellani, Lucas Gabriel
Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Wegener, Caren
Schlüter, Andreas
Pühler, Alfred
Lagares, Antonio
Brom, Susana
Pistorio, Mariano
Niehaus, Karsten
Torres Tejerizo, Gonzalo Arturo
author_role author
author2 Luchetti, Abril
Nilsson, Juliet Fernanda
Pérez Giménez, Julieta
Wegener, Caren
Schlüter, Andreas
Pühler, Alfred
Lagares, Antonio
Brom, Susana
Pistorio, Mariano
Niehaus, Karsten
Torres Tejerizo, Gonzalo Arturo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Botánica
Rhizobia
Symbiosis
Alfalfa
Exopolysaccharide
Nitrogen fixation
topic Biología
Botánica
Rhizobia
Symbiosis
Alfalfa
Exopolysaccharide
Nitrogen fixation
dc.description.none.fl_txt_mv One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.
Instituto de Biotecnologia y Biologia Molecular
description One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/124226
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dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1664-462X
info:eu-repo/semantics/altIdentifier/pmid/33643369
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.642576
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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