Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa
- Autores
- Castellani, Lucas Gabriel; Luchetti, Abril; Nilsson, Juliet Fernanda; Pérez Giménez, Julieta; Wegener, Caren; Schlüter, Andreas; Pühler, Alfred; Lagares, Antonio; Brom, Susana; Pistorio, Mariano; Niehaus, Karsten; Torres Tejerizo, Gonzalo Arturo
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.
Instituto de Biotecnologia y Biologia Molecular - Materia
-
Biología
Botánica
Rhizobia
Symbiosis
Alfalfa
Exopolysaccharide
Nitrogen fixation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/124226
Ver los metadatos del registro completo
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Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfaCastellani, Lucas GabrielLuchetti, AbrilNilsson, Juliet FernandaPérez Giménez, JulietaWegener, CarenSchlüter, AndreasPühler, AlfredLagares, AntonioBrom, SusanaPistorio, MarianoNiehaus, KarstenTorres Tejerizo, Gonzalo ArturoBiologíaBotánicaRhizobiaSymbiosisAlfalfaExopolysaccharideNitrogen fixationOne of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis.Instituto de Biotecnologia y Biologia Molecular2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/124226enginfo:eu-repo/semantics/altIdentifier/issn/1664-462Xinfo:eu-repo/semantics/altIdentifier/pmid/33643369info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.642576info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:48Zoai:sedici.unlp.edu.ar:10915/124226Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:48.381SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
title |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
spellingShingle |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa Castellani, Lucas Gabriel Biología Botánica Rhizobia Symbiosis Alfalfa Exopolysaccharide Nitrogen fixation |
title_short |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
title_full |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
title_fullStr |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
title_full_unstemmed |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
title_sort |
Exopolysaccharide characterization of Rhizobium favelukesii LPU83 and its role in the symbiosis with alfalfa |
dc.creator.none.fl_str_mv |
Castellani, Lucas Gabriel Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Wegener, Caren Schlüter, Andreas Pühler, Alfred Lagares, Antonio Brom, Susana Pistorio, Mariano Niehaus, Karsten Torres Tejerizo, Gonzalo Arturo |
author |
Castellani, Lucas Gabriel |
author_facet |
Castellani, Lucas Gabriel Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Wegener, Caren Schlüter, Andreas Pühler, Alfred Lagares, Antonio Brom, Susana Pistorio, Mariano Niehaus, Karsten Torres Tejerizo, Gonzalo Arturo |
author_role |
author |
author2 |
Luchetti, Abril Nilsson, Juliet Fernanda Pérez Giménez, Julieta Wegener, Caren Schlüter, Andreas Pühler, Alfred Lagares, Antonio Brom, Susana Pistorio, Mariano Niehaus, Karsten Torres Tejerizo, Gonzalo Arturo |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Biología Botánica Rhizobia Symbiosis Alfalfa Exopolysaccharide Nitrogen fixation |
topic |
Biología Botánica Rhizobia Symbiosis Alfalfa Exopolysaccharide Nitrogen fixation |
dc.description.none.fl_txt_mv |
One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis. Instituto de Biotecnologia y Biologia Molecular |
description |
One of the greatest inputs of available nitrogen into the biosphere occurs through the biological N2-fixation to ammonium as result of the symbiosis between rhizobia and leguminous plants. These interactions allow increased crop yields on nitrogen-poor soils. Exopolysaccharides (EPS) are key components for the establishment of an effective symbiosis between alfalfa and Ensifer meliloti, as bacteria that lack EPS are unable to infect the host plants. Rhizobium favelukesii LPU83 is an acid-tolerant rhizobia strain capable of nodulating alfalfa but inefficient to fix nitrogen. Aiming to identify the molecular determinants that allow R. favelukesii to infect plants, we studied its EPS biosynthesis. LPU83 produces an EPS I identical to the one present in Ensifer meliloti, but the organization of the genes involved in its synthesis is different. The main gene cluster needed for the synthesis of EPS I in E. meliloti, is split into three different sections in R. favelukesii, which probably arose by a recent event of horizontal gene transfer. A R. favelukesii strain devoided of all the genes needed for the synthesis of EPS I is still able to infect and nodulate alfalfa, suggesting that attention should be directed to other molecules involved in the development of the symbiosis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/124226 |
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http://sedici.unlp.edu.ar/handle/10915/124226 |
dc.language.none.fl_str_mv |
eng |
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eng |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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