Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress

Autores
Nilsson, Juliet Fernanda; Castellani, Lucas Gabriel; Draghi, Walter Omar; Mogro, Ezequiel Gerardo; Wibberg, Daniel; Winkler, Anika; Hansen, Lars; Schluter, Andreas; Pühler, Alfred; Kalinowski, Jörn; Torres Tejerizo, Gonzalo Arturo; Pistorio, Mariano
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acidic environments naturally occur worldwide and inappropriate agricultural management may also cause acidification of soils. Low soil pH values are an important barrier in the plant-rhizobia interaction. Acidic conditions disturb the establishment of the efficient rhizobia usually used as biofertilizer. This negative effect on the rhizobia-legume symbiosis is mainly due to the low acid tolerance of the bacteria. Here, we describe the identification of relevant factors in the acid tolerance of Rhizobium favelukesii using transcriptome sequencing. A total of 1924 genes were differentially expressed under acidic conditions, with ∼60% underexpressed. Rhizobium favelukesii acid response mainly includes changes in the energy metabolism and protein turnover, as well as a combination of mechanisms that may contribute to this phenotype, including GABA and histidine metabolism, cell envelope modifications and reverse proton efflux. We confirmed the acid-sensitive phenotype of a mutant in the braD gene, which showed higher expression under acid stress. Remarkably, 60% of the coding sequences encoded in the symbiotic plasmid were underexpressed and we evidenced that a strain cured for this plasmid featured an improved performance under acidic conditions. Hence, this work provides relevant information in the characterization of genes associated with tolerance or adaptation to acidic stress of R. favelukesii.
Fil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Draghi, Walter Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Mogro, Ezequiel Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Wibberg, Daniel. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Winkler, Anika. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Hansen, Lars. Universidad de Copenhagen; Dinamarca
Fil: Schluter, Andreas. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Pühler, Alfred. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Kalinowski, Jörn. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
ACID STRESS
RHIZOBIA
RNA-SEQ
TRANSCRIPTOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163868
Acceder
id CONICETDig_d4632d0c950fa0f3075ca78569db8946
oai_identifier_str oai:ri.conicet.gov.ar:11336/163868
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stressNilsson, Juliet FernandaCastellani, Lucas GabrielDraghi, Walter OmarMogro, Ezequiel GerardoWibberg, DanielWinkler, AnikaHansen, LarsSchluter, AndreasPühler, AlfredKalinowski, JörnTorres Tejerizo, Gonzalo ArturoPistorio, MarianoACID STRESSRHIZOBIARNA-SEQTRANSCRIPTOMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acidic environments naturally occur worldwide and inappropriate agricultural management may also cause acidification of soils. Low soil pH values are an important barrier in the plant-rhizobia interaction. Acidic conditions disturb the establishment of the efficient rhizobia usually used as biofertilizer. This negative effect on the rhizobia-legume symbiosis is mainly due to the low acid tolerance of the bacteria. Here, we describe the identification of relevant factors in the acid tolerance of Rhizobium favelukesii using transcriptome sequencing. A total of 1924 genes were differentially expressed under acidic conditions, with ∼60% underexpressed. Rhizobium favelukesii acid response mainly includes changes in the energy metabolism and protein turnover, as well as a combination of mechanisms that may contribute to this phenotype, including GABA and histidine metabolism, cell envelope modifications and reverse proton efflux. We confirmed the acid-sensitive phenotype of a mutant in the braD gene, which showed higher expression under acid stress. Remarkably, 60% of the coding sequences encoded in the symbiotic plasmid were underexpressed and we evidenced that a strain cured for this plasmid featured an improved performance under acidic conditions. Hence, this work provides relevant information in the characterization of genes associated with tolerance or adaptation to acidic stress of R. favelukesii.Fil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Draghi, Walter Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Mogro, Ezequiel Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Wibberg, Daniel. Universitat Bielefeld. Center For Biotechnology; AlemaniaFil: Winkler, Anika. Universitat Bielefeld. Center For Biotechnology; AlemaniaFil: Hansen, Lars. Universidad de Copenhagen; DinamarcaFil: Schluter, Andreas. Universitat Bielefeld. Center For Biotechnology; AlemaniaFil: Pühler, Alfred. Universitat Bielefeld. Center For Biotechnology; AlemaniaFil: Kalinowski, Jörn. Universitat Bielefeld. Center For Biotechnology; AlemaniaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163868Nilsson, Juliet Fernanda; Castellani, Lucas Gabriel; Draghi, Walter Omar; Mogro, Ezequiel Gerardo; Wibberg, Daniel; et al.; Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 97; 1; 11-2020; 1-160168-6496CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/97/1/fiaa235/5998221?login=falseinfo:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiaa235info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:21Zoai:ri.conicet.gov.ar:11336/163868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:21.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
title Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
spellingShingle Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
Nilsson, Juliet Fernanda
ACID STRESS
RHIZOBIA
RNA-SEQ
TRANSCRIPTOMICS
title_short Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
title_full Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
title_fullStr Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
title_full_unstemmed Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
title_sort Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress
dc.creator.none.fl_str_mv Nilsson, Juliet Fernanda
Castellani, Lucas Gabriel
Draghi, Walter Omar
Mogro, Ezequiel Gerardo
Wibberg, Daniel
Winkler, Anika
Hansen, Lars
Schluter, Andreas
Pühler, Alfred
Kalinowski, Jörn
Torres Tejerizo, Gonzalo Arturo
Pistorio, Mariano
author Nilsson, Juliet Fernanda
author_facet Nilsson, Juliet Fernanda
Castellani, Lucas Gabriel
Draghi, Walter Omar
Mogro, Ezequiel Gerardo
Wibberg, Daniel
Winkler, Anika
Hansen, Lars
Schluter, Andreas
Pühler, Alfred
Kalinowski, Jörn
Torres Tejerizo, Gonzalo Arturo
Pistorio, Mariano
author_role author
author2 Castellani, Lucas Gabriel
Draghi, Walter Omar
Mogro, Ezequiel Gerardo
Wibberg, Daniel
Winkler, Anika
Hansen, Lars
Schluter, Andreas
Pühler, Alfred
Kalinowski, Jörn
Torres Tejerizo, Gonzalo Arturo
Pistorio, Mariano
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACID STRESS
RHIZOBIA
RNA-SEQ
TRANSCRIPTOMICS
topic ACID STRESS
RHIZOBIA
RNA-SEQ
TRANSCRIPTOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acidic environments naturally occur worldwide and inappropriate agricultural management may also cause acidification of soils. Low soil pH values are an important barrier in the plant-rhizobia interaction. Acidic conditions disturb the establishment of the efficient rhizobia usually used as biofertilizer. This negative effect on the rhizobia-legume symbiosis is mainly due to the low acid tolerance of the bacteria. Here, we describe the identification of relevant factors in the acid tolerance of Rhizobium favelukesii using transcriptome sequencing. A total of 1924 genes were differentially expressed under acidic conditions, with ∼60% underexpressed. Rhizobium favelukesii acid response mainly includes changes in the energy metabolism and protein turnover, as well as a combination of mechanisms that may contribute to this phenotype, including GABA and histidine metabolism, cell envelope modifications and reverse proton efflux. We confirmed the acid-sensitive phenotype of a mutant in the braD gene, which showed higher expression under acid stress. Remarkably, 60% of the coding sequences encoded in the symbiotic plasmid were underexpressed and we evidenced that a strain cured for this plasmid featured an improved performance under acidic conditions. Hence, this work provides relevant information in the characterization of genes associated with tolerance or adaptation to acidic stress of R. favelukesii.
Fil: Nilsson, Juliet Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Draghi, Walter Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Mogro, Ezequiel Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Wibberg, Daniel. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Winkler, Anika. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Hansen, Lars. Universidad de Copenhagen; Dinamarca
Fil: Schluter, Andreas. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Pühler, Alfred. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Kalinowski, Jörn. Universitat Bielefeld. Center For Biotechnology; Alemania
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Acidic environments naturally occur worldwide and inappropriate agricultural management may also cause acidification of soils. Low soil pH values are an important barrier in the plant-rhizobia interaction. Acidic conditions disturb the establishment of the efficient rhizobia usually used as biofertilizer. This negative effect on the rhizobia-legume symbiosis is mainly due to the low acid tolerance of the bacteria. Here, we describe the identification of relevant factors in the acid tolerance of Rhizobium favelukesii using transcriptome sequencing. A total of 1924 genes were differentially expressed under acidic conditions, with ∼60% underexpressed. Rhizobium favelukesii acid response mainly includes changes in the energy metabolism and protein turnover, as well as a combination of mechanisms that may contribute to this phenotype, including GABA and histidine metabolism, cell envelope modifications and reverse proton efflux. We confirmed the acid-sensitive phenotype of a mutant in the braD gene, which showed higher expression under acid stress. Remarkably, 60% of the coding sequences encoded in the symbiotic plasmid were underexpressed and we evidenced that a strain cured for this plasmid featured an improved performance under acidic conditions. Hence, this work provides relevant information in the characterization of genes associated with tolerance or adaptation to acidic stress of R. favelukesii.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163868
Nilsson, Juliet Fernanda; Castellani, Lucas Gabriel; Draghi, Walter Omar; Mogro, Ezequiel Gerardo; Wibberg, Daniel; et al.; Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 97; 1; 11-2020; 1-16
0168-6496
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163868
identifier_str_mv Nilsson, Juliet Fernanda; Castellani, Lucas Gabriel; Draghi, Walter Omar; Mogro, Ezequiel Gerardo; Wibberg, Daniel; et al.; Global transcriptome analysis of Rhizobium favelukesii LPU83 in response to acid stress; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 97; 1; 11-2020; 1-16
0168-6496
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/97/1/fiaa235/5998221?login=false
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiaa235
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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