Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress
- Autores
- Giannuzzi, Leda; Krock, Bernd; Crettaz Minaglia, Melina Celeste; Rosso, Lorena; Houghton, Christian; Sedan, Daniela; Malanga, Gabriela; Espinosa, Mariela; Andrinolo, Darío; Hernando, Marcelo
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Microcystis are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells to low(23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth ratewas significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidencewith the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell−1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell−1 on day 2 and 70 fg.cell−1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
Biología
catalase
Chlorophyll
Microcystin variants
ROS
Toxinas Biológicas - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/56853
Ver los metadatos del registro completo
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Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stressGiannuzzi, LedaKrock, BerndCrettaz Minaglia, Melina CelesteRosso, LorenaHoughton, ChristianSedan, DanielaMalanga, GabrielaEspinosa, MarielaAndrinolo, DaríoHernando, MarceloCiencias ExactasBiologíacatalaseChlorophyllMicrocystin variantsROSToxinas Biológicas<i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells to low(23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth ratewas significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidencewith the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell−1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell−1 on day 2 and 70 fg.cell−1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.Facultad de Ciencias Exactas2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf22-30http://sedici.unlp.edu.ar/handle/10915/56853enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1532045616300734info:eu-repo/semantics/altIdentifier/issn/1532-0456info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2016.07.001info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:38:50Zoai:sedici.unlp.edu.ar:10915/56853Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:38:50.636SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| spellingShingle |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress Giannuzzi, Leda Ciencias Exactas Biología catalase Chlorophyll Microcystin variants ROS Toxinas Biológicas |
| title_short |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_full |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_fullStr |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_full_unstemmed |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_sort |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| dc.creator.none.fl_str_mv |
Giannuzzi, Leda Krock, Bernd Crettaz Minaglia, Melina Celeste Rosso, Lorena Houghton, Christian Sedan, Daniela Malanga, Gabriela Espinosa, Mariela Andrinolo, Darío Hernando, Marcelo |
| author |
Giannuzzi, Leda |
| author_facet |
Giannuzzi, Leda Krock, Bernd Crettaz Minaglia, Melina Celeste Rosso, Lorena Houghton, Christian Sedan, Daniela Malanga, Gabriela Espinosa, Mariela Andrinolo, Darío Hernando, Marcelo |
| author_role |
author |
| author2 |
Krock, Bernd Crettaz Minaglia, Melina Celeste Rosso, Lorena Houghton, Christian Sedan, Daniela Malanga, Gabriela Espinosa, Mariela Andrinolo, Darío Hernando, Marcelo |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Exactas Biología catalase Chlorophyll Microcystin variants ROS Toxinas Biológicas |
| topic |
Ciencias Exactas Biología catalase Chlorophyll Microcystin variants ROS Toxinas Biológicas |
| dc.description.none.fl_txt_mv |
<i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells to low(23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth ratewas significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidencewith the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell−1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell−1 on day 2 and 70 fg.cell−1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production. Facultad de Ciencias Exactas |
| description |
<i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells to low(23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth ratewas significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidencewith the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell−1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell−1 on day 2 and 70 fg.cell−1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production. |
| publishDate |
2016 |
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2016 |
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eng |
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