Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium...

Autores
Nieto, Esteban Emanuel; Macchi, Marianela; Valacco, María P.; Festa, Sabrina; Morelli, Irma Susana; Coppotelli, Bibiana Marina
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Universidad de Buenos Aires
Comisión de Investigaciones Científicas de la provincia de Buenos Aires
Materia
Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/157726
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/157726
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortiumNieto, Esteban EmanuelMacchi, MarianelaValacco, María P.Festa, SabrinaMorelli, Irma SusanaCoppotelli, Bibiana MarinaBiologíaQuímicaSynthetic bacterial consortiumPhenanthrene (PHN) degradation pathwayMicrobial degradation potentialBioaugmentationBioremediationPolycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesUniversidad de Buenos AiresComisión de Investigaciones Científicas de la provincia de Buenos Aires2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf181-197http://sedici.unlp.edu.ar/handle/10915/157726enginfo:eu-repo/semantics/altIdentifier/issn/1572-9729info:eu-repo/semantics/altIdentifier/doi/10.1007/s10532-022-10012-3info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:41:06Zoai:sedici.unlp.edu.ar:10915/157726Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:41:06.762SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
spellingShingle Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
Nieto, Esteban Emanuel
Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
title_short Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_full Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_fullStr Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_full_unstemmed Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
title_sort Metaproteomic and gene expression analysis of interspecies interactions in a PAH‑degrading synthetic microbial consortium constructed with the key microbes of a natural consortium
dc.creator.none.fl_str_mv Nieto, Esteban Emanuel
Macchi, Marianela
Valacco, María P.
Festa, Sabrina
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author Nieto, Esteban Emanuel
author_facet Nieto, Esteban Emanuel
Macchi, Marianela
Valacco, María P.
Festa, Sabrina
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author_role author
author2 Macchi, Marianela
Valacco, María P.
Festa, Sabrina
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
topic Biología
Química
Synthetic bacterial consortium
Phenanthrene (PHN) degradation pathway
Microbial degradation potential
Bioaugmentation
Bioremediation
dc.description.none.fl_txt_mv Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Universidad de Buenos Aires
Comisión de Investigaciones Científicas de la provincia de Buenos Aires
description Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/157726
url http://sedici.unlp.edu.ar/handle/10915/157726
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1572-9729
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10532-022-10012-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
181-197
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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