Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation

Autores
Vazquez, Susana Claudia; Nogales, B.; Ruberto, Lucas Adolfo Mauro; Mestre, C.; Christie Oleza, J; Ferrero, Marcela Alejandra; Bosch, R; Mac Cormack, Walter Patricio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Antarctica, the environmental conditions and the restrictions imposed by the Antarctic Treaty prevent inoculation with foreign bacteria. Therefore, our aim was to investigate native bacterial consortia which might serve to design bacterial formulas suitable for soil bioremediation processes at cold temperatures. Two bacterial consortia, M10 and J13, were isolated from diesel contaminated Antarctic soils. Their ability to use hydrocarbons was evaluated in vitro and by the detection of three catabolic genes (alkB, nahAc, xylE). Both consortia showed similar 16S rRNA gene profiles, suggesting the presence of the same phylotypes. Total 16S rDNA was cloned from M10 grown on diesel. Sixty clones were screened, grouped by restriction profiles of PCR-amplified inserts and sequenced. T-RFLP (Terminal-Restriction-Fragment-Length-Polymorphism) of clones showed that all phylotypes from the entire consortia were recovered. A culture-dependent approach was used to isolate M10 components able to utilise aliphatic and aromatic hydrocarbons. Pseudomonas, Stenotrophomonas, Pedobacter and Brevundimonas genera were detected. The combination of dependent and independent culture methods allowed elucidating the taxonomic composition of these native bacterial consortia. Further work will assess whether combining the isolates obtained as a defined mixed culture can enhance bioremediation of contaminated soils.
Fil: Vazquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina;
Fil: Nogales, B.. Universidad de las Islas Baleares; España;
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina;
Fil: Mestre, C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina; Universidad Nacional del Litoral; Argentina;
Fil: Christie Oleza, J. Universidad de las Islas Baleares; España;
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;
Fil: Bosch, R. Universidad de las Islas Baleares; España;
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina;
Materia
Bacterial Consortium
Antarctica
T-Rflp
Bioremediation
Hydrocarbon Degradation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1701
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1701
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentationVazquez, Susana ClaudiaNogales, B.Ruberto, Lucas Adolfo MauroMestre, C.Christie Oleza, JFerrero, Marcela AlejandraBosch, RMac Cormack, Walter PatricioBacterial ConsortiumAntarcticaT-RflpBioremediationHydrocarbon Degradationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Antarctica, the environmental conditions and the restrictions imposed by the Antarctic Treaty prevent inoculation with foreign bacteria. Therefore, our aim was to investigate native bacterial consortia which might serve to design bacterial formulas suitable for soil bioremediation processes at cold temperatures. Two bacterial consortia, M10 and J13, were isolated from diesel contaminated Antarctic soils. Their ability to use hydrocarbons was evaluated in vitro and by the detection of three catabolic genes (alkB, nahAc, xylE). Both consortia showed similar 16S rRNA gene profiles, suggesting the presence of the same phylotypes. Total 16S rDNA was cloned from M10 grown on diesel. Sixty clones were screened, grouped by restriction profiles of PCR-amplified inserts and sequenced. T-RFLP (Terminal-Restriction-Fragment-Length-Polymorphism) of clones showed that all phylotypes from the entire consortia were recovered. A culture-dependent approach was used to isolate M10 components able to utilise aliphatic and aromatic hydrocarbons. Pseudomonas, Stenotrophomonas, Pedobacter and Brevundimonas genera were detected. The combination of dependent and independent culture methods allowed elucidating the taxonomic composition of these native bacterial consortia. Further work will assess whether combining the isolates obtained as a defined mixed culture can enhance bioremediation of contaminated soils.Fil: Vazquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina;Fil: Nogales, B.. Universidad de las Islas Baleares; España;Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina;Fil: Mestre, C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina; Universidad Nacional del Litoral; Argentina;Fil: Christie Oleza, J. Universidad de las Islas Baleares; España;Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;Fil: Bosch, R. Universidad de las Islas Baleares; España;Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina;Elsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1701Vazquez, Susana Claudia; Nogales, B.; Ruberto, Lucas Adolfo Mauro; Mestre, C.; Christie Oleza, J; et al.; Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation; Elsevier; International Biodeterioration & Biodegradation; 77; 1-2013; 22-300964-8305enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830512002867info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.11.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:25:03Zoai:ri.conicet.gov.ar:11336/1701instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:25:03.933CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
title Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
spellingShingle Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
Vazquez, Susana Claudia
Bacterial Consortium
Antarctica
T-Rflp
Bioremediation
Hydrocarbon Degradation
title_short Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
title_full Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
title_fullStr Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
title_full_unstemmed Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
title_sort Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation
dc.creator.none.fl_str_mv Vazquez, Susana Claudia
Nogales, B.
Ruberto, Lucas Adolfo Mauro
Mestre, C.
Christie Oleza, J
Ferrero, Marcela Alejandra
Bosch, R
Mac Cormack, Walter Patricio
author Vazquez, Susana Claudia
author_facet Vazquez, Susana Claudia
Nogales, B.
Ruberto, Lucas Adolfo Mauro
Mestre, C.
Christie Oleza, J
Ferrero, Marcela Alejandra
Bosch, R
Mac Cormack, Walter Patricio
author_role author
author2 Nogales, B.
Ruberto, Lucas Adolfo Mauro
Mestre, C.
Christie Oleza, J
Ferrero, Marcela Alejandra
Bosch, R
Mac Cormack, Walter Patricio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial Consortium
Antarctica
T-Rflp
Bioremediation
Hydrocarbon Degradation
topic Bacterial Consortium
Antarctica
T-Rflp
Bioremediation
Hydrocarbon Degradation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Antarctica, the environmental conditions and the restrictions imposed by the Antarctic Treaty prevent inoculation with foreign bacteria. Therefore, our aim was to investigate native bacterial consortia which might serve to design bacterial formulas suitable for soil bioremediation processes at cold temperatures. Two bacterial consortia, M10 and J13, were isolated from diesel contaminated Antarctic soils. Their ability to use hydrocarbons was evaluated in vitro and by the detection of three catabolic genes (alkB, nahAc, xylE). Both consortia showed similar 16S rRNA gene profiles, suggesting the presence of the same phylotypes. Total 16S rDNA was cloned from M10 grown on diesel. Sixty clones were screened, grouped by restriction profiles of PCR-amplified inserts and sequenced. T-RFLP (Terminal-Restriction-Fragment-Length-Polymorphism) of clones showed that all phylotypes from the entire consortia were recovered. A culture-dependent approach was used to isolate M10 components able to utilise aliphatic and aromatic hydrocarbons. Pseudomonas, Stenotrophomonas, Pedobacter and Brevundimonas genera were detected. The combination of dependent and independent culture methods allowed elucidating the taxonomic composition of these native bacterial consortia. Further work will assess whether combining the isolates obtained as a defined mixed culture can enhance bioremediation of contaminated soils.
Fil: Vazquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina;
Fil: Nogales, B.. Universidad de las Islas Baleares; España;
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina;
Fil: Mestre, C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Biotecnología; Argentina; Universidad Nacional del Litoral; Argentina;
Fil: Christie Oleza, J. Universidad de las Islas Baleares; España;
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;
Fil: Bosch, R. Universidad de las Islas Baleares; España;
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina;
description In Antarctica, the environmental conditions and the restrictions imposed by the Antarctic Treaty prevent inoculation with foreign bacteria. Therefore, our aim was to investigate native bacterial consortia which might serve to design bacterial formulas suitable for soil bioremediation processes at cold temperatures. Two bacterial consortia, M10 and J13, were isolated from diesel contaminated Antarctic soils. Their ability to use hydrocarbons was evaluated in vitro and by the detection of three catabolic genes (alkB, nahAc, xylE). Both consortia showed similar 16S rRNA gene profiles, suggesting the presence of the same phylotypes. Total 16S rDNA was cloned from M10 grown on diesel. Sixty clones were screened, grouped by restriction profiles of PCR-amplified inserts and sequenced. T-RFLP (Terminal-Restriction-Fragment-Length-Polymorphism) of clones showed that all phylotypes from the entire consortia were recovered. A culture-dependent approach was used to isolate M10 components able to utilise aliphatic and aromatic hydrocarbons. Pseudomonas, Stenotrophomonas, Pedobacter and Brevundimonas genera were detected. The combination of dependent and independent culture methods allowed elucidating the taxonomic composition of these native bacterial consortia. Further work will assess whether combining the isolates obtained as a defined mixed culture can enhance bioremediation of contaminated soils.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1701
Vazquez, Susana Claudia; Nogales, B.; Ruberto, Lucas Adolfo Mauro; Mestre, C.; Christie Oleza, J; et al.; Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation; Elsevier; International Biodeterioration & Biodegradation; 77; 1-2013; 22-30
0964-8305
url http://hdl.handle.net/11336/1701
identifier_str_mv Vazquez, Susana Claudia; Nogales, B.; Ruberto, Lucas Adolfo Mauro; Mestre, C.; Christie Oleza, J; et al.; Characterization of bacterial consortia from diesel-contaminated Antarctic soils: Towards the design of tailored formulas for bioaugmentation; Elsevier; International Biodeterioration & Biodegradation; 77; 1-2013; 22-30
0964-8305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830512002867
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.11.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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