Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil

Autores
Festa, Sabrina; Coppotelli, Bibiana Marina; Morelli, Irma Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A phenanthrene-degrading consortium (CON-Phe) was obtained from a chronically contaminated soil. The consortium degraded 58% of the phenanthrene supplied during the first 7 days of incubation with the concomitant accumulation of 1-hydroxy-2-naphthoic acid (HNA). The composition of CON-Phe and its dynamic during phenanthrene degradation were determined using culture-dependent and independent approaches (polymerase chain reaction-denaturing gradient gel electrophoresis and clone libraries). Among the detected bacteria by both methods, Sphingomonadaceae family frequently occurred, but some genera were only observed through culture-dependent methods (Enterobacter sp. and Pseudomonas sp.) and others only through culture-independent methods (Ochrobacterium sp., Alcaligenes sp.). Five different strains were isolated and identified; between them only the strain AM (Sphingobium sp.) showed phenanthrene degradation. And only in strains AM and B (Enterobacter sp) evidence of the presence of PAH-ring hydroxylating dioxygenases genes was found. In order to determine the role of the isolated strains in the CON-Phe and the interaction between them, the phenanthrene degradation by defined mixed cultures was studied. All the defined consortia, formed by strain AM together with another of the isolated strains, showed percentages of phenanthrene biodegradation significantly higher than the strain AM alone and the natural consortia. In addition, no accumulation of HNA was observed in these defined consortia. These results might suggest that in the soil consortia the competition between the species and the community dynamics could cause a negative effect in the phenanthrene degradation. On the other hand, a synergistic effect between the phenanthrene-degrading strain AM and the other isolated strains was observed.
Fil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
BACTERIAL CONSORTIUM
DGGE
PHENANTHRENE DEGRADATION
SPHINGOMONADACEAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93468
Acceder
id CONICETDig_56cd0def81d57582422cd5a24b9f5eac
oai_identifier_str oai:ri.conicet.gov.ar:11336/93468
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soilFesta, SabrinaCoppotelli, Bibiana MarinaMorelli, Irma SusanaBACTERIAL CONSORTIUMDGGEPHENANTHRENE DEGRADATIONSPHINGOMONADACEAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A phenanthrene-degrading consortium (CON-Phe) was obtained from a chronically contaminated soil. The consortium degraded 58% of the phenanthrene supplied during the first 7 days of incubation with the concomitant accumulation of 1-hydroxy-2-naphthoic acid (HNA). The composition of CON-Phe and its dynamic during phenanthrene degradation were determined using culture-dependent and independent approaches (polymerase chain reaction-denaturing gradient gel electrophoresis and clone libraries). Among the detected bacteria by both methods, Sphingomonadaceae family frequently occurred, but some genera were only observed through culture-dependent methods (Enterobacter sp. and Pseudomonas sp.) and others only through culture-independent methods (Ochrobacterium sp., Alcaligenes sp.). Five different strains were isolated and identified; between them only the strain AM (Sphingobium sp.) showed phenanthrene degradation. And only in strains AM and B (Enterobacter sp) evidence of the presence of PAH-ring hydroxylating dioxygenases genes was found. In order to determine the role of the isolated strains in the CON-Phe and the interaction between them, the phenanthrene degradation by defined mixed cultures was studied. All the defined consortia, formed by strain AM together with another of the isolated strains, showed percentages of phenanthrene biodegradation significantly higher than the strain AM alone and the natural consortia. In addition, no accumulation of HNA was observed in these defined consortia. These results might suggest that in the soil consortia the competition between the species and the community dynamics could cause a negative effect in the phenanthrene degradation. On the other hand, a synergistic effect between the phenanthrene-degrading strain AM and the other isolated strains was observed.Fil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93468Festa, Sabrina; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil; Elsevier; International Biodeterioration and Biodegradation; 85; 11-2013; 42-510964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830513002199info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2013.06.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:37Zoai:ri.conicet.gov.ar:11336/93468instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:38.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
title Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
spellingShingle Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
Festa, Sabrina
BACTERIAL CONSORTIUM
DGGE
PHENANTHRENE DEGRADATION
SPHINGOMONADACEAE
title_short Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
title_full Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
title_fullStr Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
title_full_unstemmed Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
title_sort Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil
dc.creator.none.fl_str_mv Festa, Sabrina
Coppotelli, Bibiana Marina
Morelli, Irma Susana
author Festa, Sabrina
author_facet Festa, Sabrina
Coppotelli, Bibiana Marina
Morelli, Irma Susana
author_role author
author2 Coppotelli, Bibiana Marina
Morelli, Irma Susana
author2_role author
author
dc.subject.none.fl_str_mv BACTERIAL CONSORTIUM
DGGE
PHENANTHRENE DEGRADATION
SPHINGOMONADACEAE
topic BACTERIAL CONSORTIUM
DGGE
PHENANTHRENE DEGRADATION
SPHINGOMONADACEAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A phenanthrene-degrading consortium (CON-Phe) was obtained from a chronically contaminated soil. The consortium degraded 58% of the phenanthrene supplied during the first 7 days of incubation with the concomitant accumulation of 1-hydroxy-2-naphthoic acid (HNA). The composition of CON-Phe and its dynamic during phenanthrene degradation were determined using culture-dependent and independent approaches (polymerase chain reaction-denaturing gradient gel electrophoresis and clone libraries). Among the detected bacteria by both methods, Sphingomonadaceae family frequently occurred, but some genera were only observed through culture-dependent methods (Enterobacter sp. and Pseudomonas sp.) and others only through culture-independent methods (Ochrobacterium sp., Alcaligenes sp.). Five different strains were isolated and identified; between them only the strain AM (Sphingobium sp.) showed phenanthrene degradation. And only in strains AM and B (Enterobacter sp) evidence of the presence of PAH-ring hydroxylating dioxygenases genes was found. In order to determine the role of the isolated strains in the CON-Phe and the interaction between them, the phenanthrene degradation by defined mixed cultures was studied. All the defined consortia, formed by strain AM together with another of the isolated strains, showed percentages of phenanthrene biodegradation significantly higher than the strain AM alone and the natural consortia. In addition, no accumulation of HNA was observed in these defined consortia. These results might suggest that in the soil consortia the competition between the species and the community dynamics could cause a negative effect in the phenanthrene degradation. On the other hand, a synergistic effect between the phenanthrene-degrading strain AM and the other isolated strains was observed.
Fil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description A phenanthrene-degrading consortium (CON-Phe) was obtained from a chronically contaminated soil. The consortium degraded 58% of the phenanthrene supplied during the first 7 days of incubation with the concomitant accumulation of 1-hydroxy-2-naphthoic acid (HNA). The composition of CON-Phe and its dynamic during phenanthrene degradation were determined using culture-dependent and independent approaches (polymerase chain reaction-denaturing gradient gel electrophoresis and clone libraries). Among the detected bacteria by both methods, Sphingomonadaceae family frequently occurred, but some genera were only observed through culture-dependent methods (Enterobacter sp. and Pseudomonas sp.) and others only through culture-independent methods (Ochrobacterium sp., Alcaligenes sp.). Five different strains were isolated and identified; between them only the strain AM (Sphingobium sp.) showed phenanthrene degradation. And only in strains AM and B (Enterobacter sp) evidence of the presence of PAH-ring hydroxylating dioxygenases genes was found. In order to determine the role of the isolated strains in the CON-Phe and the interaction between them, the phenanthrene degradation by defined mixed cultures was studied. All the defined consortia, formed by strain AM together with another of the isolated strains, showed percentages of phenanthrene biodegradation significantly higher than the strain AM alone and the natural consortia. In addition, no accumulation of HNA was observed in these defined consortia. These results might suggest that in the soil consortia the competition between the species and the community dynamics could cause a negative effect in the phenanthrene degradation. On the other hand, a synergistic effect between the phenanthrene-degrading strain AM and the other isolated strains was observed.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93468
Festa, Sabrina; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil; Elsevier; International Biodeterioration and Biodegradation; 85; 11-2013; 42-51
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93468
identifier_str_mv Festa, Sabrina; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil; Elsevier; International Biodeterioration and Biodegradation; 85; 11-2013; 42-51
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830513002199
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2013.06.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614255262826496
score 13.070432

Publicaciones similares