Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular cha...

Autores
Brentassi, María Eugenia; Franco, Mario Emilio Ernesto; Balatti, Pedro Alberto; Medina, Rocío; Bernabei, Franco; Marino de Remes Lenicov, Ana María
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “Candidatus Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of D. maidis. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “Candidatus Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of D. maidis showed that the sequence of Sulcia accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others Sulcia described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of Sulcia in the bacteriomes of D. maidis was discussed.
Facultad de Ciencias Naturales y Museo
Centro de Investigaciones en Fitopatología
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Naturales
Ciencias Exactas
"Candidatus Sulcia muelleri"
Bacteriomes
Corn stunt
Corn leafhopper
Obligate endosymbionts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136965
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/136965
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmissionBrentassi, María EugeniaFranco, Mario Emilio ErnestoBalatti, Pedro AlbertoMedina, RocíoBernabei, FrancoMarino de Remes Lenicov, Ana MaríaCiencias NaturalesCiencias Exactas"Candidatus Sulcia muelleri"BacteriomesCorn stuntCorn leafhopperObligate endosymbiontsIn this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper <i>Dalbulus maidis</i> by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “<i>Candidatus</i> Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of <i>D. maidis</i>. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “<i>Candidatus</i> Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of <i>D. maidis</i> showed that the sequence of <i>Sulcia</i> accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others <i>Sulcia</i> described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of <i>Sulcia</i> in the bacteriomes of <i>D. maidis</i> was discussed.Facultad de Ciencias Naturales y MuseoCentro de Investigaciones en FitopatologíaCentro de Investigación y Desarrollo en Fermentaciones Industriales2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1421-1429http://sedici.unlp.edu.ar/handle/10915/136965enginfo:eu-repo/semantics/altIdentifier/issn/1615-6102info:eu-repo/semantics/altIdentifier/issn/0033-183Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-016-1033-4info:eu-repo/semantics/altIdentifier/pmid/27730310info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:13:26Zoai:sedici.unlp.edu.ar:10915/136965Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:13:27.197SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
spellingShingle Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
Brentassi, María Eugenia
Ciencias Naturales
Ciencias Exactas
"Candidatus Sulcia muelleri"
Bacteriomes
Corn stunt
Corn leafhopper
Obligate endosymbionts
title_short Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_full Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_fullStr Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_full_unstemmed Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_sort Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
dc.creator.none.fl_str_mv Brentassi, María Eugenia
Franco, Mario Emilio Ernesto
Balatti, Pedro Alberto
Medina, Rocío
Bernabei, Franco
Marino de Remes Lenicov, Ana María
author Brentassi, María Eugenia
author_facet Brentassi, María Eugenia
Franco, Mario Emilio Ernesto
Balatti, Pedro Alberto
Medina, Rocío
Bernabei, Franco
Marino de Remes Lenicov, Ana María
author_role author
author2 Franco, Mario Emilio Ernesto
Balatti, Pedro Alberto
Medina, Rocío
Bernabei, Franco
Marino de Remes Lenicov, Ana María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Ciencias Exactas
"Candidatus Sulcia muelleri"
Bacteriomes
Corn stunt
Corn leafhopper
Obligate endosymbionts
topic Ciencias Naturales
Ciencias Exactas
"Candidatus Sulcia muelleri"
Bacteriomes
Corn stunt
Corn leafhopper
Obligate endosymbionts
dc.description.none.fl_txt_mv In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper <i>Dalbulus maidis</i> by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “<i>Candidatus</i> Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of <i>D. maidis</i>. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “<i>Candidatus</i> Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of <i>D. maidis</i> showed that the sequence of <i>Sulcia</i> accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others <i>Sulcia</i> described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of <i>Sulcia</i> in the bacteriomes of <i>D. maidis</i> was discussed.
Facultad de Ciencias Naturales y Museo
Centro de Investigaciones en Fitopatología
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper <i>Dalbulus maidis</i> by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “<i>Candidatus</i> Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of <i>D. maidis</i>. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “<i>Candidatus</i> Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of <i>D. maidis</i> showed that the sequence of <i>Sulcia</i> accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others <i>Sulcia</i> described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of <i>Sulcia</i> in the bacteriomes of <i>D. maidis</i> was discussed.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136965
url http://sedici.unlp.edu.ar/handle/10915/136965
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1615-6102
info:eu-repo/semantics/altIdentifier/issn/0033-183X
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-016-1033-4
info:eu-repo/semantics/altIdentifier/pmid/27730310
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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1421-1429
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reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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