Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization...

Autores: Brentassi, María Eugenia; Franco, Mario Emilio Ernesto; Balatti, Pedro Alberto; Medina, Rocío; Bernabei, Franco; Marino de Remes Lenicov, Ana Maria
Año de publicación: 2016
Idioma: español castellano
Tipo de recurso: artículo
Estado: versión enviada
Descripción: In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont Candidatus Sulcia muelleri (Phylum Bacteroidetes) resides in bacteriomes of D. maidis. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as Candidatus Nasuia deltocephalinicola, a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of D. maidis showed that the sequence of Sulcia accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others Sulcia described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of Sulcia in the bacteriomes of D. maidis was discussed.
Materia: Agronomía, reproducción y protección de plantas
Candidatus Sulcia mueller
Bacteriomes
Corn stun
Corn leafhopper
Obligate endosymbionts
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
Institución: Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador: oai:digital.cic.gba.gob.ar:11746/7188

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repository_id_str	9441
network_name_str	CIC Digital (CICBA)
spelling	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmissionBrentassi, María EugeniaFranco, Mario Emilio ErnestoBalatti, Pedro AlbertoMedina, RocíoBernabei, FrancoMarino de Remes Lenicov, Ana MariaAgronomía, reproducción y protección de plantasCandidatus Sulcia muellerBacteriomesCorn stunCorn leafhopperObligate endosymbiontsIn this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont Candidatus Sulcia muelleri (Phylum Bacteroidetes) resides in bacteriomes of D. maidis. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as Candidatus Nasuia deltocephalinicola, a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of D. maidis showed that the sequence of Sulcia accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others Sulcia described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of Sulcia in the bacteriomes of D. maidis was discussed.2016-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/7188spainfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-016-1033-4info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:07Zoai:digital.cic.gba.gob.ar:11746/7188Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:07.446CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
title	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
spellingShingle	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission Brentassi, María Eugenia Agronomía, reproducción y protección de plantas Candidatus Sulcia mueller Bacteriomes Corn stun Corn leafhopper Obligate endosymbionts
title_short	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
title_full	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
title_fullStr	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
title_full_unstemmed	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
title_sort	Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure and transovarial transmission
dc.creator.none.fl_str_mv	Brentassi, María Eugenia Franco, Mario Emilio Ernesto Balatti, Pedro Alberto Medina, Rocío Bernabei, Franco Marino de Remes Lenicov, Ana Maria
author	Brentassi, María Eugenia
author_facet	Brentassi, María Eugenia Franco, Mario Emilio Ernesto Balatti, Pedro Alberto Medina, Rocío Bernabei, Franco Marino de Remes Lenicov, Ana Maria
author_role	author
author2	Franco, Mario Emilio Ernesto Balatti, Pedro Alberto Medina, Rocío Bernabei, Franco Marino de Remes Lenicov, Ana Maria
author2_role	author author author author author
dc.subject.none.fl_str_mv	Agronomía, reproducción y protección de plantas Candidatus Sulcia mueller Bacteriomes Corn stun Corn leafhopper Obligate endosymbionts
topic	Agronomía, reproducción y protección de plantas Candidatus Sulcia mueller Bacteriomes Corn stun Corn leafhopper Obligate endosymbionts
dc.description.none.fl_txt_mv	In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont Candidatus Sulcia muelleri (Phylum Bacteroidetes) resides in bacteriomes of D. maidis. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as Candidatus Nasuia deltocephalinicola, a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of D. maidis showed that the sequence of Sulcia accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others Sulcia described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of Sulcia in the bacteriomes of D. maidis was discussed.
description	In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont Candidatus Sulcia muelleri (Phylum Bacteroidetes) resides in bacteriomes of D. maidis. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as Candidatus Nasuia deltocephalinicola, a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of D. maidis showed that the sequence of Sulcia accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others Sulcia described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of Sulcia in the bacteriomes of D. maidis was discussed.
publishDate	2016
dc.date.none.fl_str_mv	2016-05-09
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	submittedVersion
dc.identifier.none.fl_str_mv	https://digital.cic.gba.gob.ar/handle/11746/7188
url	https://digital.cic.gba.gob.ar/handle/11746/7188
dc.language.none.fl_str_mv	spa
language	spa
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1007/s00709-016-1033-4
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc/4.0/
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA
reponame_str	CIC Digital (CICBA)
collection	CIC Digital (CICBA)
instname_str	Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str	CICBA
institution	CICBA
repository.name.fl_str_mv	CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv	marisa.degiusti@sedici.unlp.edu.ar
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Bacteriomes of the corn leaf hopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization...

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