First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii

Autores
Foieri, Alvaro; Decker Franco, Cecilia; Marino de Remes Lenicov, Ana María; Arneodo Larochette, Joel Demian
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. ‘Candidatus Zinderia insecticola’ or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marino de Remes Lenicov, Ana Maria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina
Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fuente
Bulletin of Insectology 75 (1) : 15-20 (junio 2022)
Materia
Bacteria
Cercopidae
Symbionts
Phylogeny
South America
Simbiontico
Filogenia
América del Sur
Bacteriome
Candidatus Sulcia muelleri
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/12347
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spelling First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchiiFoieri, AlvaroDecker Franco, CeciliaMarino de Remes Lenicov, Ana MaríaArneodo Larochette, Joel DemianBacteriaCercopidaeSymbiontsPhylogenySouth AmericaSimbionticoFilogeniaAmérica del SurBacteriomeCandidatus Sulcia muelleriSpittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. ‘Candidatus Zinderia insecticola’ or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Marino de Remes Lenicov, Ana Maria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; ArgentinaFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaAlma Mater Studiorum, Università di Bologna2022-07-19T15:37:27Z2022-07-19T15:37:27Z2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12347http://www.bulletinofinsectology.org/pdfarticles/vol75-2022-015-020foieri.pdf2283-03321721-8861Bulletin of Insectology 75 (1) : 15-20 (junio 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:37Zoai:localhost:20.500.12123/12347instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:37.858INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
spellingShingle First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
Foieri, Alvaro
Bacteria
Cercopidae
Symbionts
Phylogeny
South America
Simbiontico
Filogenia
América del Sur
Bacteriome
Candidatus Sulcia muelleri
title_short First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_full First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_fullStr First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_full_unstemmed First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_sort First identification of bacterial endosymbionts in three South-American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
dc.creator.none.fl_str_mv Foieri, Alvaro
Decker Franco, Cecilia
Marino de Remes Lenicov, Ana María
Arneodo Larochette, Joel Demian
author Foieri, Alvaro
author_facet Foieri, Alvaro
Decker Franco, Cecilia
Marino de Remes Lenicov, Ana María
Arneodo Larochette, Joel Demian
author_role author
author2 Decker Franco, Cecilia
Marino de Remes Lenicov, Ana María
Arneodo Larochette, Joel Demian
author2_role author
author
author
dc.subject.none.fl_str_mv Bacteria
Cercopidae
Symbionts
Phylogeny
South America
Simbiontico
Filogenia
América del Sur
Bacteriome
Candidatus Sulcia muelleri
topic Bacteria
Cercopidae
Symbionts
Phylogeny
South America
Simbiontico
Filogenia
América del Sur
Bacteriome
Candidatus Sulcia muelleri
dc.description.none.fl_txt_mv Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. ‘Candidatus Zinderia insecticola’ or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Foieri, Alvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marino de Remes Lenicov, Ana Maria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina
Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont ‘Candidatus Sulcia muelleri’ (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. ‘Candidatus Zinderia insecticola’ or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-19T15:37:27Z
2022-07-19T15:37:27Z
2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12347
http://www.bulletinofinsectology.org/pdfarticles/vol75-2022-015-020foieri.pdf
2283-0332
1721-8861
url http://hdl.handle.net/20.500.12123/12347
http://www.bulletinofinsectology.org/pdfarticles/vol75-2022-015-020foieri.pdf
identifier_str_mv 2283-0332
1721-8861
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Alma Mater Studiorum, Università di Bologna
publisher.none.fl_str_mv Alma Mater Studiorum, Università di Bologna
dc.source.none.fl_str_mv Bulletin of Insectology 75 (1) : 15-20 (junio 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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