Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture

Autores
Barrio, Daniel Alejandro; Williams, Patricia Ana María; Cortizo, Ana María; Etcheverry, Susana B.
Año de publicación
2003
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vanadium compounds show interesting biological and pharmacological properties. Some of them display insulin-mimetic effects and others produce antitumor actions. The bioactivity of vanadium is present in inorganic species like the vanadyl(IV) cation or vanadate( V) anion. Nevertheless, the development of new vanadium derivatives with organic ligands which improve the beneficial actions and decrease the toxic effects is of great interest. On the other hand, the mechanisms involved in vanadium bioactivity are still poorly understood. A new vanadium complex of the vanadyl(IV) cation with the disaccharide trehalose (TreVO), Na6 [VO(Tre)2].4H2O, here reported, shows interesting insulin- mimetic properties in two osteoblast cell lines, a normal one (MC3T3E1) and a tumoral one (UMR106). The complex affected the proliferation of both cell lines in a different manner. On tumoral cells, TreVO caused a weak stimulation of growth at 5 lM but it inhibited cell proliferation in a dose-response manner between 50 and 100 lM. TreVO significantly inhibited UMR106 differentiation (15–25% of basal) in the range 5–100 lM. On normal osteoblasts, TreVO behaved as a mitogen at 5–25 lM. Different inhibitors of the MAPK pathway blocked this effect. At higher concentrations (75–100 lM), the complex was a weak inhibitor of the MC3T3E1 proliferation. Besides, TreVO enhanced glucose consumption by a mechanism independent of the PI3-kinase activation. In both cell lines, TreVO stimulated the ERK phosphorylation in a dose- and time-dependent manner. Different inhibitors (PD98059, wortmannin, vitamins C and E) partially decreased this effect, which was totally inhibited by their combination. These results suggest that TreVO could be a potential candidate for therapeutic treatments.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium
Insulina
Bioquímica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/76081

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spelling Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in cultureBarrio, Daniel AlejandroWilliams, Patricia Ana MaríaCortizo, Ana MaríaEtcheverry, Susana B.Ciencias Exactasdiabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadiumInsulinaBioquímicaVanadium compounds show interesting biological and pharmacological properties. Some of them display insulin-mimetic effects and others produce antitumor actions. The bioactivity of vanadium is present in inorganic species like the vanadyl(IV) cation or vanadate( V) anion. Nevertheless, the development of new vanadium derivatives with organic ligands which improve the beneficial actions and decrease the toxic effects is of great interest. On the other hand, the mechanisms involved in vanadium bioactivity are still poorly understood. A new vanadium complex of the vanadyl(IV) cation with the disaccharide trehalose (TreVO), Na6 [VO(Tre)2].4H2O, here reported, shows interesting insulin- mimetic properties in two osteoblast cell lines, a normal one (MC3T3E1) and a tumoral one (UMR106). The complex affected the proliferation of both cell lines in a different manner. On tumoral cells, TreVO caused a weak stimulation of growth at 5 lM but it inhibited cell proliferation in a dose-response manner between 50 and 100 lM. TreVO significantly inhibited UMR106 differentiation (15–25% of basal) in the range 5–100 lM. On normal osteoblasts, TreVO behaved as a mitogen at 5–25 lM. Different inhibitors of the MAPK pathway blocked this effect. At higher concentrations (75–100 lM), the complex was a weak inhibitor of the MC3T3E1 proliferation. Besides, TreVO enhanced glucose consumption by a mechanism independent of the PI3-kinase activation. In both cell lines, TreVO stimulated the ERK phosphorylation in a dose- and time-dependent manner. Different inhibitors (PD98059, wortmannin, vitamins C and E) partially decreased this effect, which was totally inhibited by their combination. These results suggest that TreVO could be a potential candidate for therapeutic treatments.Facultad de Ciencias Exactas2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf459-468http://sedici.unlp.edu.ar/handle/10915/76081spainfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-002-0438-zinfo:eu-repo/semantics/altIdentifier/hdl/11746/4373info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:13:20Zoai:sedici.unlp.edu.ar:10915/76081Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:13:20.684SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
spellingShingle Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
Barrio, Daniel Alejandro
Ciencias Exactas
diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium
Insulina
Bioquímica
title_short Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_full Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_fullStr Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_full_unstemmed Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
title_sort Synthesis of a new vanadyl(IV) complex with trehalose (TreVO): insulin-mimetic activities in osteoblast-like cells in culture
dc.creator.none.fl_str_mv Barrio, Daniel Alejandro
Williams, Patricia Ana María
Cortizo, Ana María
Etcheverry, Susana B.
author Barrio, Daniel Alejandro
author_facet Barrio, Daniel Alejandro
Williams, Patricia Ana María
Cortizo, Ana María
Etcheverry, Susana B.
author_role author
author2 Williams, Patricia Ana María
Cortizo, Ana María
Etcheverry, Susana B.
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium
Insulina
Bioquímica
topic Ciencias Exactas
diabetes mellitus; insulin mimics; osteoblasts; signal transduction; vanadium
Insulina
Bioquímica
dc.description.none.fl_txt_mv Vanadium compounds show interesting biological and pharmacological properties. Some of them display insulin-mimetic effects and others produce antitumor actions. The bioactivity of vanadium is present in inorganic species like the vanadyl(IV) cation or vanadate( V) anion. Nevertheless, the development of new vanadium derivatives with organic ligands which improve the beneficial actions and decrease the toxic effects is of great interest. On the other hand, the mechanisms involved in vanadium bioactivity are still poorly understood. A new vanadium complex of the vanadyl(IV) cation with the disaccharide trehalose (TreVO), Na6 [VO(Tre)2].4H2O, here reported, shows interesting insulin- mimetic properties in two osteoblast cell lines, a normal one (MC3T3E1) and a tumoral one (UMR106). The complex affected the proliferation of both cell lines in a different manner. On tumoral cells, TreVO caused a weak stimulation of growth at 5 lM but it inhibited cell proliferation in a dose-response manner between 50 and 100 lM. TreVO significantly inhibited UMR106 differentiation (15–25% of basal) in the range 5–100 lM. On normal osteoblasts, TreVO behaved as a mitogen at 5–25 lM. Different inhibitors of the MAPK pathway blocked this effect. At higher concentrations (75–100 lM), the complex was a weak inhibitor of the MC3T3E1 proliferation. Besides, TreVO enhanced glucose consumption by a mechanism independent of the PI3-kinase activation. In both cell lines, TreVO stimulated the ERK phosphorylation in a dose- and time-dependent manner. Different inhibitors (PD98059, wortmannin, vitamins C and E) partially decreased this effect, which was totally inhibited by their combination. These results suggest that TreVO could be a potential candidate for therapeutic treatments.
Facultad de Ciencias Exactas
description Vanadium compounds show interesting biological and pharmacological properties. Some of them display insulin-mimetic effects and others produce antitumor actions. The bioactivity of vanadium is present in inorganic species like the vanadyl(IV) cation or vanadate( V) anion. Nevertheless, the development of new vanadium derivatives with organic ligands which improve the beneficial actions and decrease the toxic effects is of great interest. On the other hand, the mechanisms involved in vanadium bioactivity are still poorly understood. A new vanadium complex of the vanadyl(IV) cation with the disaccharide trehalose (TreVO), Na6 [VO(Tre)2].4H2O, here reported, shows interesting insulin- mimetic properties in two osteoblast cell lines, a normal one (MC3T3E1) and a tumoral one (UMR106). The complex affected the proliferation of both cell lines in a different manner. On tumoral cells, TreVO caused a weak stimulation of growth at 5 lM but it inhibited cell proliferation in a dose-response manner between 50 and 100 lM. TreVO significantly inhibited UMR106 differentiation (15–25% of basal) in the range 5–100 lM. On normal osteoblasts, TreVO behaved as a mitogen at 5–25 lM. Different inhibitors of the MAPK pathway blocked this effect. At higher concentrations (75–100 lM), the complex was a weak inhibitor of the MC3T3E1 proliferation. Besides, TreVO enhanced glucose consumption by a mechanism independent of the PI3-kinase activation. In both cell lines, TreVO stimulated the ERK phosphorylation in a dose- and time-dependent manner. Different inhibitors (PD98059, wortmannin, vitamins C and E) partially decreased this effect, which was totally inhibited by their combination. These results suggest that TreVO could be a potential candidate for therapeutic treatments.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
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format article
status_str publishedVersion
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
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