Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants

Autores
Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Duarte, Evelyn Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus
Fuente
African Journal of Biotechnology, 2016, vol. 15, no. 25, p. 1292-1298.
Materia
Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/27788
Acceder
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oai_identifier_str oai:repositorio.unne.edu.ar:123456789/27788
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explantsDuarte, Evelyn RaquelSansberro, Pedro AlfonsoLuna, Claudia VerónicaAxillary shootsAdventitious shootsMicropropagationSeedUncoated seedsCotyledonStem nodesBlack lapachoFil: Duarte, Evelyn Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllusAcademic Journals2016-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfDuarte, Evelyn Raquel, Sansberro, Pedro Alfonso y Luna, Claudia Verónica, 2016. Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants. African Journal of Biotechnology. Nairobi: Academic Journals, vol. 15, no. 25, p. 1292-1298. ISSN 1684-5315.1684-5315http://repositorio.unne.edu.ar/handle/123456789/27788African Journal of Biotechnology, 2016, vol. 15, no. 25, p. 1292-1298.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://academicjournals.org/journal/AJB/article-abstract/059F2CD59041info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-04T11:13:18Zoai:repositorio.unne.edu.ar:123456789/27788instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-04 11:13:18.243Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
spellingShingle Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
Duarte, Evelyn Raquel
Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
title_short Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_full Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_fullStr Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_full_unstemmed Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
title_sort Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants
dc.creator.none.fl_str_mv Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author Duarte, Evelyn Raquel
author_facet Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author_role author
author2 Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author2_role author
author
dc.subject.none.fl_str_mv Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
topic Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
dc.description.none.fl_txt_mv Fil: Duarte, Evelyn Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus
description Fil: Duarte, Evelyn Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Duarte, Evelyn Raquel, Sansberro, Pedro Alfonso y Luna, Claudia Verónica, 2016. Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants. African Journal of Biotechnology. Nairobi: Academic Journals, vol. 15, no. 25, p. 1292-1298. ISSN 1684-5315.
1684-5315
http://repositorio.unne.edu.ar/handle/123456789/27788
identifier_str_mv Duarte, Evelyn Raquel, Sansberro, Pedro Alfonso y Luna, Claudia Verónica, 2016. Micropropagation of handroanthus heptaphyllus (Vell.) mattos from seedling explants. African Journal of Biotechnology. Nairobi: Academic Journals, vol. 15, no. 25, p. 1292-1298. ISSN 1684-5315.
1684-5315
url http://repositorio.unne.edu.ar/handle/123456789/27788
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://academicjournals.org/journal/AJB/article-abstract/059F2CD59041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv African Journal of Biotechnology, 2016, vol. 15, no. 25, p. 1292-1298.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.623145

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