Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

Autores
Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.
Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
Handroanthus Heptaphyllus
Axillary Shoots
Adventitious Shoots
Micropropagation
Seed
Uncoated Seeds
Cotyledon
Stem Nodes
Black Lapacho
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20648
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explantsDuarte, Evelyn RaquelSansberro, Pedro AlfonsoLuna, Claudia VerónicaHandroanthus HeptaphyllusAxillary ShootsAdventitious ShootsMicropropagationSeedUncoated SeedsCotyledonStem NodesBlack Lapachohttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaAcademic Journals2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20648Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants; Academic Journals; African Journal of Biotechnology; 15; 25; 6-2016; 1292-12981684-5315CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5897/AJB2015.15194info:eu-repo/semantics/altIdentifier/url/http://academicjournals.org/journal/AJB/article-abstract/059F2CD59041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:16Zoai:ri.conicet.gov.ar:11336/20648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:16.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
title Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
spellingShingle Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
Duarte, Evelyn Raquel
Handroanthus Heptaphyllus
Axillary Shoots
Adventitious Shoots
Micropropagation
Seed
Uncoated Seeds
Cotyledon
Stem Nodes
Black Lapacho
title_short Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
title_full Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
title_fullStr Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
title_full_unstemmed Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
title_sort Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
dc.creator.none.fl_str_mv Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author Duarte, Evelyn Raquel
author_facet Duarte, Evelyn Raquel
Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author_role author
author2 Sansberro, Pedro Alfonso
Luna, Claudia Verónica
author2_role author
author
dc.subject.none.fl_str_mv Handroanthus Heptaphyllus
Axillary Shoots
Adventitious Shoots
Micropropagation
Seed
Uncoated Seeds
Cotyledon
Stem Nodes
Black Lapacho
topic Handroanthus Heptaphyllus
Axillary Shoots
Adventitious Shoots
Micropropagation
Seed
Uncoated Seeds
Cotyledon
Stem Nodes
Black Lapacho
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.
Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20648
Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants; Academic Journals; African Journal of Biotechnology; 15; 25; 6-2016; 1292-1298
1684-5315
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20648
identifier_str_mv Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants; Academic Journals; African Journal of Biotechnology; 15; 25; 6-2016; 1292-1298
1684-5315
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5897/AJB2015.15194
info:eu-repo/semantics/altIdentifier/url/http://academicjournals.org/journal/AJB/article-abstract/059F2CD59041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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