Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants
- Autores
- Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.
Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina - Materia
-
Handroanthus Heptaphyllus
Axillary Shoots
Adventitious Shoots
Micropropagation
Seed
Uncoated Seeds
Cotyledon
Stem Nodes
Black Lapacho - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/20648
Ver los metadatos del registro completo
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Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explantsDuarte, Evelyn RaquelSansberro, Pedro AlfonsoLuna, Claudia VerónicaHandroanthus HeptaphyllusAxillary ShootsAdventitious ShootsMicropropagationSeedUncoated SeedsCotyledonStem NodesBlack Lapachohttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaAcademic Journals2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20648Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants; Academic Journals; African Journal of Biotechnology; 15; 25; 6-2016; 1292-12981684-5315CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5897/AJB2015.15194info:eu-repo/semantics/altIdentifier/url/http://academicjournals.org/journal/AJB/article-abstract/059F2CD59041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:15Zoai:ri.conicet.gov.ar:11336/20648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:15.303CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
title |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
spellingShingle |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants Duarte, Evelyn Raquel Handroanthus Heptaphyllus Axillary Shoots Adventitious Shoots Micropropagation Seed Uncoated Seeds Cotyledon Stem Nodes Black Lapacho |
title_short |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
title_full |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
title_fullStr |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
title_full_unstemmed |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
title_sort |
Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants |
dc.creator.none.fl_str_mv |
Duarte, Evelyn Raquel Sansberro, Pedro Alfonso Luna, Claudia Verónica |
author |
Duarte, Evelyn Raquel |
author_facet |
Duarte, Evelyn Raquel Sansberro, Pedro Alfonso Luna, Claudia Verónica |
author_role |
author |
author2 |
Sansberro, Pedro Alfonso Luna, Claudia Verónica |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Handroanthus Heptaphyllus Axillary Shoots Adventitious Shoots Micropropagation Seed Uncoated Seeds Cotyledon Stem Nodes Black Lapacho |
topic |
Handroanthus Heptaphyllus Axillary Shoots Adventitious Shoots Micropropagation Seed Uncoated Seeds Cotyledon Stem Nodes Black Lapacho |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus. Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina |
description |
Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/20648 Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants; Academic Journals; African Journal of Biotechnology; 15; 25; 6-2016; 1292-1298 1684-5315 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/20648 |
identifier_str_mv |
Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica; Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants; Academic Journals; African Journal of Biotechnology; 15; 25; 6-2016; 1292-1298 1684-5315 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.5897/AJB2015.15194 info:eu-repo/semantics/altIdentifier/url/http://academicjournals.org/journal/AJB/article-abstract/059F2CD59041 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Journals |
publisher.none.fl_str_mv |
Academic Journals |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268779841585152 |
score |
13.13397 |