Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants

Autores
Espasandin, Fabiana Daniela; Calzadilla, Pablo I.; Maiale, Santiago Javier; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.
Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Calzadilla, Pablo I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina.
Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina.
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina.
Fuente
J Plant Growth Regul, 2018, no. 37, p. 156-165.
Materia
Putrescine
Abscisic acid
Nced
Salt tolerance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/9120
Acceder
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oai_identifier_str oai:repositorio.unne.edu.ar:123456789/9120
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plantsEspasandin, Fabiana DanielaCalzadilla, Pablo I.Maiale, Santiago JavierRuiz, Oscar AdolfoSansberro, Pedro AlfonsoPutrescineAbscisic acidNcedSalt toleranceTo analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Calzadilla, Pablo I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina.Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina.Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina.Springer Science2017-05-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 156-165application/pdfEspasandin, Fabiana D., et. al., 2018. Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants. J Plant Growth Regul. New York: Springer Science, no. 37, p. 156-165. ISSN 1435-8107.0721-7595http://repositorio.unne.edu.ar/handle/123456789/9120J Plant Growth Regul, 2018, no. 37, p. 156-165.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://link.springer.com/content/pdf/10.1007/s00344-017-9713-7.pdfhttps://link.springer.com/article/10.1007/s00344-017-9713-7http://dx.doi.org/10.1007/s00344-017-9713-7info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-10-16T10:07:07Zoai:repositorio.unne.edu.ar:123456789/9120instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-10-16 10:07:07.857Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
spellingShingle Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
Espasandin, Fabiana Daniela
Putrescine
Abscisic acid
Nced
Salt tolerance
title_short Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_full Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_fullStr Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_full_unstemmed Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_sort Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
dc.creator.none.fl_str_mv Espasandin, Fabiana Daniela
Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author Espasandin, Fabiana Daniela
author_facet Espasandin, Fabiana Daniela
Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_role author
author2 Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author2_role author
author
author
author
dc.subject.none.fl_str_mv Putrescine
Abscisic acid
Nced
Salt tolerance
topic Putrescine
Abscisic acid
Nced
Salt tolerance
dc.description.none.fl_txt_mv To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.
Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Calzadilla, Pablo I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina.
Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina.
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina.
description To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Espasandin, Fabiana D., et. al., 2018. Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants. J Plant Growth Regul. New York: Springer Science, no. 37, p. 156-165. ISSN 1435-8107.
0721-7595
http://repositorio.unne.edu.ar/handle/123456789/9120
identifier_str_mv Espasandin, Fabiana D., et. al., 2018. Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants. J Plant Growth Regul. New York: Springer Science, no. 37, p. 156-165. ISSN 1435-8107.
0721-7595
url http://repositorio.unne.edu.ar/handle/123456789/9120
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://link.springer.com/content/pdf/10.1007/s00344-017-9713-7.pdf
https://link.springer.com/article/10.1007/s00344-017-9713-7
http://dx.doi.org/10.1007/s00344-017-9713-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 156-165
application/pdf
dc.publisher.none.fl_str_mv Springer Science
publisher.none.fl_str_mv Springer Science
dc.source.none.fl_str_mv J Plant Growth Regul, 2018, no. 37, p. 156-165.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1846145998276001792
score 12.712165

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