Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

Autores
Fusco, Luciano S.; Neto, Emidio B.; Francisco, Aleff F.; Alfonso, Jorge; Soares, Andreimar; Pimenta, Daniel C.; Leiva, Laura C.
Año de publicación
2020
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Fusco, Luciano S. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Neto, Emidio B. Instituto Butantan; Brasil.
Fil: Francisco, Aleff F. Sao Paulo State University; Brazil.
Fil: Alfonso, Jorge. Universidade Federal de Rondonia; Brazil.
Fil: Soares, Andreimar. Universidade Federal de Rondonia; Brazil.
Fil: Soares, Andreimar. Centro Universitario Sao Lucas; Brazil.
Fil: Pimenta, Daniel C. Instituto Butantan; Brasil.
Fil: Leiva, Laura C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Leiva, Laura C. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 50 -nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
Fuente
Toxicon: X, 2020, vol. 7, 100047
Materia
Crotalus durissus terrificus
Northeastern Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/58047
Acceder
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oai_identifier_str oai:repositorio.unne.edu.ar:123456789/58047
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Fast venomic analysis of Crotalus durissus terrificus from northeastern ArgentinaFusco, Luciano S.Neto, Emidio B.Francisco, Aleff F.Alfonso, JorgeSoares, AndreimarPimenta, Daniel C.Leiva, Laura C.Crotalus durissus terrificusNortheastern ArgentinaFil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Fil: Fusco, Luciano S. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Fil: Neto, Emidio B. Instituto Butantan; Brasil.Fil: Francisco, Aleff F. Sao Paulo State University; Brazil.Fil: Alfonso, Jorge. Universidade Federal de Rondonia; Brazil.Fil: Soares, Andreimar. Universidade Federal de Rondonia; Brazil.Fil: Soares, Andreimar. Centro Universitario Sao Lucas; Brazil.Fil: Pimenta, Daniel C. Instituto Butantan; Brasil.Fil: Leiva, Laura C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Fil: Leiva, Laura C. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 50 -nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.Elsevier Ltd.2020-09-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf100047application/pdfFusco, Luciano S., et. al, 2020. Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina. Toxicon: X: Países BajosElsevier Ltd., 100047, ISSN 041-0101.0041-0101http://repositorio.unne.edu.ar/handle/123456789/58047Toxicon: X, 2020, vol. 7, 100047reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordestespahttps://doi.org/10.1016/j.toxcx.2020.100047info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-04T11:14:04Zoai:repositorio.unne.edu.ar:123456789/58047instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-04 11:14:05.024Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
spellingShingle Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
Fusco, Luciano S.
Crotalus durissus terrificus
Northeastern Argentina
title_short Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_fullStr Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full_unstemmed Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_sort Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
dc.creator.none.fl_str_mv Fusco, Luciano S.
Neto, Emidio B.
Francisco, Aleff F.
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel C.
Leiva, Laura C.
author Fusco, Luciano S.
author_facet Fusco, Luciano S.
Neto, Emidio B.
Francisco, Aleff F.
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel C.
Leiva, Laura C.
author_role author
author2 Neto, Emidio B.
Francisco, Aleff F.
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel C.
Leiva, Laura C.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Crotalus durissus terrificus
Northeastern Argentina
topic Crotalus durissus terrificus
Northeastern Argentina
dc.description.none.fl_txt_mv Fil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Fusco, Luciano S. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Neto, Emidio B. Instituto Butantan; Brasil.
Fil: Francisco, Aleff F. Sao Paulo State University; Brazil.
Fil: Alfonso, Jorge. Universidade Federal de Rondonia; Brazil.
Fil: Soares, Andreimar. Universidade Federal de Rondonia; Brazil.
Fil: Soares, Andreimar. Centro Universitario Sao Lucas; Brazil.
Fil: Pimenta, Daniel C. Instituto Butantan; Brasil.
Fil: Leiva, Laura C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Leiva, Laura C. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 50 -nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
description Fil: Fusco, Luciano S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Fusco, Luciano S., et. al, 2020. Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina. Toxicon: X: Países BajosElsevier Ltd., 100047, ISSN 041-0101.
0041-0101
http://repositorio.unne.edu.ar/handle/123456789/58047
identifier_str_mv Fusco, Luciano S., et. al, 2020. Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina. Toxicon: X: Países BajosElsevier Ltd., 100047, ISSN 041-0101.
0041-0101
url http://repositorio.unne.edu.ar/handle/123456789/58047
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.toxcx.2020.100047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
100047
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv Toxicon: X, 2020, vol. 7, 100047
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.623145

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