Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

Autores
Fusco, Luciano Sebastian; Neto, Emidio B.; Francisco, Aleff F.; Alfonso, Jorge; Soares, Andreimar; Pimenta, Daniel C.; Leiva, Laura Cristina Ana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Neto, Emidio B.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Francisco, Aleff F.. Instituto de Biociencias; Brasil
Fil: Alfonso, Jorge. Fundación Oswaldo Cruz; Brasil
Fil: Soares, Andreimar. Fundación Oswaldo Cruz; Brasil
Fil: Pimenta, Daniel C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Materia
VENOM
CROTALUS DIRISSUS TERRIFICUS
FAST VENOMIC ANALYSIS
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141222
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/141222
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fast venomic analysis of Crotalus durissus terrificus from northeastern ArgentinaFusco, Luciano SebastianNeto, Emidio B.Francisco, Aleff F.Alfonso, JorgeSoares, AndreimarPimenta, Daniel C.Leiva, Laura Cristina AnaVENOMCROTALUS DIRISSUS TERRIFICUSFAST VENOMIC ANALYSISARGENTINAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Neto, Emidio B.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Francisco, Aleff F.. Instituto de Biociencias; BrasilFil: Alfonso, Jorge. Fundación Oswaldo Cruz; BrasilFil: Soares, Andreimar. Fundación Oswaldo Cruz; BrasilFil: Pimenta, Daniel C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaElsevier2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141222Fusco, Luciano Sebastian; Neto, Emidio B.; Francisco, Aleff F.; Alfonso, Jorge; Soares, Andreimar; et al.; Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina; Elsevier; Toxicon: X; 7; 9-2020; 1-72590-1710CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2590171020300254info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxcx.2020.100047info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:43Zoai:ri.conicet.gov.ar:11336/141222instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:43.562CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
spellingShingle Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
Fusco, Luciano Sebastian
VENOM
CROTALUS DIRISSUS TERRIFICUS
FAST VENOMIC ANALYSIS
ARGENTINA
title_short Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_fullStr Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full_unstemmed Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_sort Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
dc.creator.none.fl_str_mv Fusco, Luciano Sebastian
Neto, Emidio B.
Francisco, Aleff F.
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel C.
Leiva, Laura Cristina Ana
author Fusco, Luciano Sebastian
author_facet Fusco, Luciano Sebastian
Neto, Emidio B.
Francisco, Aleff F.
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel C.
Leiva, Laura Cristina Ana
author_role author
author2 Neto, Emidio B.
Francisco, Aleff F.
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel C.
Leiva, Laura Cristina Ana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv VENOM
CROTALUS DIRISSUS TERRIFICUS
FAST VENOMIC ANALYSIS
ARGENTINA
topic VENOM
CROTALUS DIRISSUS TERRIFICUS
FAST VENOMIC ANALYSIS
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Neto, Emidio B.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Francisco, Aleff F.. Instituto de Biociencias; Brasil
Fil: Alfonso, Jorge. Fundación Oswaldo Cruz; Brasil
Fil: Soares, Andreimar. Fundación Oswaldo Cruz; Brasil
Fil: Pimenta, Daniel C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
description The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141222
Fusco, Luciano Sebastian; Neto, Emidio B.; Francisco, Aleff F.; Alfonso, Jorge; Soares, Andreimar; et al.; Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina; Elsevier; Toxicon: X; 7; 9-2020; 1-7
2590-1710
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141222
identifier_str_mv Fusco, Luciano Sebastian; Neto, Emidio B.; Francisco, Aleff F.; Alfonso, Jorge; Soares, Andreimar; et al.; Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina; Elsevier; Toxicon: X; 7; 9-2020; 1-7
2590-1710
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2590171020300254
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxcx.2020.100047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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