Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage

Autores
Gay, Carolina; Maruñak, Silvana; Teibler, Pamela; Leiva, Laura; Acosta, Ofelia
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Leiva, Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Acosta, Ofelia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops diporus venom, another medical relevant genus of the northeastern region of Argentina, was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice injected with B. diporus venom showed a small spot remaining even at the highest ratio of IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for revealing the central role of SVMPs in the development of myotoxicity of B. alternatus venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholi- pases A2 (PLA2s) in B. diporus venom.
Fuente
Toxicon, 2013, vol. 63, p. 104-111.
Materia
Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs SVMPs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/58049
Acceder
id RIUNNE_344fbd5b3c572f96798f7bdb20fb963c
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/58049
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damageGay, CarolinaMaruñak, SilvanaTeibler, PamelaLeiva, LauraAcosta, OfeliaBalterginBothrops alternatusHemorrhageMyotoxicityPolyclonal specific antibodiesSVMPs SVMPsFil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Fil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Leiva, Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Acosta, Ofelia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops diporus venom, another medical relevant genus of the northeastern region of Argentina, was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice injected with B. diporus venom showed a small spot remaining even at the highest ratio of IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for revealing the central role of SVMPs in the development of myotoxicity of B. alternatus venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholi- pases A2 (PLA2s) in B. diporus venom.Elsevier Ltd.2013-03-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 104-111application/pdfGay, Carolina, et. al, 2013. Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage. Toxicon: Países BajosElsevier Ltd., p. 104-111, ISSN 0041-0101.0041-0101http://repositorio.unne.edu.ar/handle/123456789/58049Toxicon, 2013, vol. 63, p. 104-111.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordestespahttp://dx.doi.org/10.1016/j.toxicon.2012.11.028info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:46Zoai:repositorio.unne.edu.ar:123456789/58049instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:46.991Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
spellingShingle Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
Gay, Carolina
Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs SVMPs
title_short Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_full Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_fullStr Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_full_unstemmed Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_sort Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
dc.creator.none.fl_str_mv Gay, Carolina
Maruñak, Silvana
Teibler, Pamela
Leiva, Laura
Acosta, Ofelia
author Gay, Carolina
author_facet Gay, Carolina
Maruñak, Silvana
Teibler, Pamela
Leiva, Laura
Acosta, Ofelia
author_role author
author2 Maruñak, Silvana
Teibler, Pamela
Leiva, Laura
Acosta, Ofelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs SVMPs
topic Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs SVMPs
dc.description.none.fl_txt_mv Fil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Teibler, Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Leiva, Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Acosta, Ofelia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops diporus venom, another medical relevant genus of the northeastern region of Argentina, was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice injected with B. diporus venom showed a small spot remaining even at the highest ratio of IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for revealing the central role of SVMPs in the development of myotoxicity of B. alternatus venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholi- pases A2 (PLA2s) in B. diporus venom.
description Fil: Gay, Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Gay, Carolina, et. al, 2013. Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage. Toxicon: Países BajosElsevier Ltd., p. 104-111, ISSN 0041-0101.
0041-0101
http://repositorio.unne.edu.ar/handle/123456789/58049
identifier_str_mv Gay, Carolina, et. al, 2013. Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage. Toxicon: Países BajosElsevier Ltd., p. 104-111, ISSN 0041-0101.
0041-0101
url http://repositorio.unne.edu.ar/handle/123456789/58049
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.toxicon.2012.11.028
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 104-111
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv Toxicon, 2013, vol. 63, p. 104-111.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1844621697073807360
score 12.559606

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