Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation

Autores
Dolce, Natalia Raquel; Medina, Ricardo Daniel; Mroginski, Luis Amado; Rey, Hebe Yolanda
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at 4,L20,L70, andL196 8C. Fertilizing ability of fresh and stored (30 to 360 days) pollinia was determined by the fruit and seed formation for each treatment, as well as by the seed viability, in vitro seed germination, and seedling growth. Pollinia stored at L70 and L196 8C showed high fertilizing ability (94.4% to 100.0%) even 1 year after collection, revealing no significant differences with fresh pollinia. Seeds from all treatments showed high viability (91.2% to 94.3%) through the 2,3,5-triphenyltetrazolium chloride (TTC) reduction assay and high in vitro germination (91.7% to 97.3%). Thus, successful ultracold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection, or precooling treatment before placing into an ultra freezer (L70 8C) or immersing in liquid nitrogen (LN) (L196 8C).
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Mroginski, Luís Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe R. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fuente
Hortscience, 2016, vol. 51, no. 10, p. 1265-1269.
Materia
Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/9170
Acceder
id RIUNNE_13aa74940eb1c30f2b3d463d16bfd8e7
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/9170
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formationDolce, Natalia RaquelMedina, Ricardo DanielMroginski, Luis AmadoRey, Hebe YolandaLong-term storageLiquid nitrogenPollenIn vitro seed germinationOrchidsPollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at 4,L20,L70, andL196 8C. Fertilizing ability of fresh and stored (30 to 360 days) pollinia was determined by the fruit and seed formation for each treatment, as well as by the seed viability, in vitro seed germination, and seedling growth. Pollinia stored at L70 and L196 8C showed high fertilizing ability (94.4% to 100.0%) even 1 year after collection, revealing no significant differences with fresh pollinia. Seeds from all treatments showed high viability (91.2% to 94.3%) through the 2,3,5-triphenyltetrazolium chloride (TTC) reduction assay and high in vitro germination (91.7% to 97.3%). Thus, successful ultracold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection, or precooling treatment before placing into an ultra freezer (L70 8C) or immersing in liquid nitrogen (LN) (L196 8C).Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Mroginski, Luís Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Rey, Hebe R. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.American Society for Horticultural Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 1265-1269application/pdfDolce, Natalia Raquel, et al., 2016. Storage of Cohniella cepula (Orchidaceae) Pollinia : Fertilizing Ability and Subsequent Fruit and Seed Formation. Hortscience. Estados Unidos: American Society for Horticultural Science, vol. 51, no 10, p. 1265–1269. ISSN 2327-9834.0018-5345http://repositorio.unne.edu.ar/handle/123456789/9170Hortscience, 2016, vol. 51, no. 10, p. 1265-1269.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://journals.ashs.org/hortsci/view/journals/hortsci/51/10/article-p1265.xml?rskey=hD6ckJinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:29:15Zoai:repositorio.unne.edu.ar:123456789/9170instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:29:16.23Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
spellingShingle Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
Dolce, Natalia Raquel
Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
title_short Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_full Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_fullStr Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_full_unstemmed Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
title_sort Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
dc.creator.none.fl_str_mv Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author Dolce, Natalia Raquel
author_facet Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_role author
author2 Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author2_role author
author
author
dc.subject.none.fl_str_mv Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
topic Long-term storage
Liquid nitrogen
Pollen
In vitro seed germination
Orchids
dc.description.none.fl_txt_mv Pollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at 4,L20,L70, andL196 8C. Fertilizing ability of fresh and stored (30 to 360 days) pollinia was determined by the fruit and seed formation for each treatment, as well as by the seed viability, in vitro seed germination, and seedling growth. Pollinia stored at L70 and L196 8C showed high fertilizing ability (94.4% to 100.0%) even 1 year after collection, revealing no significant differences with fresh pollinia. Seeds from all treatments showed high viability (91.2% to 94.3%) through the 2,3,5-triphenyltetrazolium chloride (TTC) reduction assay and high in vitro germination (91.7% to 97.3%). Thus, successful ultracold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection, or precooling treatment before placing into an ultra freezer (L70 8C) or immersing in liquid nitrogen (LN) (L196 8C).
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Mroginski, Luís Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe R. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
description Pollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at 4,L20,L70, andL196 8C. Fertilizing ability of fresh and stored (30 to 360 days) pollinia was determined by the fruit and seed formation for each treatment, as well as by the seed viability, in vitro seed germination, and seedling growth. Pollinia stored at L70 and L196 8C showed high fertilizing ability (94.4% to 100.0%) even 1 year after collection, revealing no significant differences with fresh pollinia. Seeds from all treatments showed high viability (91.2% to 94.3%) through the 2,3,5-triphenyltetrazolium chloride (TTC) reduction assay and high in vitro germination (91.7% to 97.3%). Thus, successful ultracold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection, or precooling treatment before placing into an ultra freezer (L70 8C) or immersing in liquid nitrogen (LN) (L196 8C).
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Dolce, Natalia Raquel, et al., 2016. Storage of Cohniella cepula (Orchidaceae) Pollinia : Fertilizing Ability and Subsequent Fruit and Seed Formation. Hortscience. Estados Unidos: American Society for Horticultural Science, vol. 51, no 10, p. 1265–1269. ISSN 2327-9834.
0018-5345
http://repositorio.unne.edu.ar/handle/123456789/9170
identifier_str_mv Dolce, Natalia Raquel, et al., 2016. Storage of Cohniella cepula (Orchidaceae) Pollinia : Fertilizing Ability and Subsequent Fruit and Seed Formation. Hortscience. Estados Unidos: American Society for Horticultural Science, vol. 51, no 10, p. 1265–1269. ISSN 2327-9834.
0018-5345
url http://repositorio.unne.edu.ar/handle/123456789/9170
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://journals.ashs.org/hortsci/view/journals/hortsci/51/10/article-p1265.xml?rskey=hD6ckJ
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 1265-1269
application/pdf
dc.publisher.none.fl_str_mv American Society for Horticultural Science
publisher.none.fl_str_mv American Society for Horticultural Science
dc.source.none.fl_str_mv Hortscience, 2016, vol. 51, no. 10, p. 1265-1269.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1844621659456143360
score 12.559606

Publicaciones similares