Gametogenesis in Rhinella bergi (Anura : Bufonidae) : morphological and morphometric analysis

• Autores: Cheij, Esteban Omar; Olea, Gabriela Beatriz; Rodríguez, Florencia Evelyn; Céspedez, Jorge Abel; Flores Quintana, Carolina Isabel
• Año de publicación: 2023
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Acta Zoológica, 2023, vol. 105, no. 3, p. 317-330.
• Materia: Anurans; Folliculogenesis; Reproduction; Spermatogenesis
• Institución: Universidad Nacional del Nordeste

Fil: Cheij, Esteban Omar. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Olea, Gabriela Beatriz. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Olea, Gabriela Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas; Argentina.
Fil: Olea, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina.
Fil: Rodríguez, Florencia Evelyn. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Rodríguez, Florencia Evelyn. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina.
Fil: Rodríguez, Florencia Evelyn. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Instituto de Química Básica & Aplicada del Nordeste Argentino; Argentina.
Fil: Céspedez, Jorge Abel. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Flores Quintana, Carolina Isabel. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Flores Quintana, Carolina Isabel. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas; Argentina.
This study aims to characterize the gonads, folliculogenesis, and spermatogenesis processes of male and female Rhinella bergi through anatomical, histological and morphometric analysis. We worked with specimens previously collected in the spring and summerseasons(2014–2015), in which the gonads were studied based on morphological, histological and morphometric analysis. The reproductive system was analyzed using a stereoscopic microscope and a scanning electron microscope (SEM). Histological preparations were made following the conventional protocol of dehydration, inclusion in paraffin and stained with haematoxylineosin, Mallory trichrome and PAS histochemical reactions. The anatomical and morphometric characteristics and the gametogenesis in R. bergi agree with what was proposed for other analyzed species of the Bufonidae family. Females with ovaries with oocytes in different stages of maturity and postovulatory females were found. The males presented continuous spermatogenesis. The results of this work serve as a basis for the characterization of the reproductive cycle in R. bergi and, at the same time, provide background information on the analysis of gametogenic activity. Future investigations will be focused on evaluating the reproductive cycle in this species, both in ovaries and testes as well as Bidder's organ, to compare and correlate with the results obtained in this study.