Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase

Autores
Barreiro Arcos, María Laura; Sterle, Helena Andrea; Vercelli, C.; Valli, Eduardo; Cayrol, María Florencia; Klecha, Alicia Juana; Paulazo, Maria Alejandra; Díaz Flaqué, María Celeste; Franchi, A. M.; Cremaschi, Graciela A.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Vercelli, C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biomedicina; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klecha, Alicia Juana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Flaqué, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Franchi, A. M. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Franchi, A. M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Cremaschi, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Abstract: Thyroid hormones are important regulators of cell physiology, inducing cell proliferation, differentiation or apoptosis, depending on the cell type. Thyroid hormones induce proliferation in short-term T lymphocyte cultures. In this study, we assessed the effect of long-term thyroxine (T4) treatment on the balance of proliferation and apoptosis and the intermediate participants in T lymphoma cells. Treatment with T4 affected this balance from the fifth day of culture, inhibiting proliferation in a time-dependent manner. This effect was associated with apoptosis induction, as characterized through nuclear morphological changes, DNA fragmentation, and Annexin V-FITC/Propidium Iodide co-staining. In addition, increased iNOS gene and protein levels, and enzyme activity were observed. The generation of reactive oxygen species, depolarization of the mitochondrial membrane, and a reduction in glutathione levels were also observed. The imbalance between oxidants and antioxidants species is typically associated with the nitration of proteins, including PKCζ, an isoenzyme essential for lymphoma cell division and survival. Consistently, evidence of PKCζ nitration via proteasome degradation was also observed in this study. Taken together, these results suggest that the long-term culture of T lymphoma cells with T4 induces apoptosis through the increased production of oxidative species resulting from both augmented iNOS activity and the loss of mitochondrial function. These species induce the nitration of proteins involved in cell viability, promoting proteasome degradation. Furthermore, we discuss the impact of these results on the modulation of T lymphoma growth and the thyroid status in vivo.
Fuente
Apoptosis. 2013. 18(11)
Materia
APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/15265
Acceder
id RIUCA_fb62b8a559f4c080b08decfffc4dfa05
oai_identifier_str oai:ucacris:123456789/15265
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthaseBarreiro Arcos, María LauraSterle, Helena AndreaVercelli, C.Valli, EduardoCayrol, María FlorenciaKlecha, Alicia JuanaPaulazo, Maria AlejandraDíaz Flaqué, María CelesteFranchi, A. M.Cremaschi, Graciela A.APOPTOSISADNGENESMITOCONDRIANITRATOSPROTEINASTIEMPOLINFOMA DE CELULAS TFil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Vercelli, C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biomedicina; ArgentinaFil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Klecha, Alicia Juana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Díaz Flaqué, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Franchi, A. M. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Franchi, A. M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Cremaschi, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaAbstract: Thyroid hormones are important regulators of cell physiology, inducing cell proliferation, differentiation or apoptosis, depending on the cell type. Thyroid hormones induce proliferation in short-term T lymphocyte cultures. In this study, we assessed the effect of long-term thyroxine (T4) treatment on the balance of proliferation and apoptosis and the intermediate participants in T lymphoma cells. Treatment with T4 affected this balance from the fifth day of culture, inhibiting proliferation in a time-dependent manner. This effect was associated with apoptosis induction, as characterized through nuclear morphological changes, DNA fragmentation, and Annexin V-FITC/Propidium Iodide co-staining. In addition, increased iNOS gene and protein levels, and enzyme activity were observed. The generation of reactive oxygen species, depolarization of the mitochondrial membrane, and a reduction in glutathione levels were also observed. The imbalance between oxidants and antioxidants species is typically associated with the nitration of proteins, including PKCζ, an isoenzyme essential for lymphoma cell division and survival. Consistently, evidence of PKCζ nitration via proteasome degradation was also observed in this study. Taken together, these results suggest that the long-term culture of T lymphoma cells with T4 induces apoptosis through the increased production of oxidative species resulting from both augmented iNOS activity and the loss of mitochondrial function. These species induce the nitration of proteins involved in cell viability, promoting proteasome degradation. Furthermore, we discuss the impact of these results on the modulation of T lymphoma growth and the thyroid status in vivo.Springer2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/152651573-675X (online)1360-818510.1007/s10495-013-0869-823733107Barreiro Arcos, M.L. Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase [en línea]. Apoptosis. 2013. 18(11) doi:10.1007/s10495-013-0869-8 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15265Apoptosis. 2013. 18(11)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:58:53Zoai:ucacris:123456789/15265instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:58:54.164Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
spellingShingle Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
Barreiro Arcos, María Laura
APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
title_short Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_full Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_fullStr Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_full_unstemmed Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
title_sort Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase
dc.creator.none.fl_str_mv Barreiro Arcos, María Laura
Sterle, Helena Andrea
Vercelli, C.
Valli, Eduardo
Cayrol, María Florencia
Klecha, Alicia Juana
Paulazo, Maria Alejandra
Díaz Flaqué, María Celeste
Franchi, A. M.
Cremaschi, Graciela A.
author Barreiro Arcos, María Laura
author_facet Barreiro Arcos, María Laura
Sterle, Helena Andrea
Vercelli, C.
Valli, Eduardo
Cayrol, María Florencia
Klecha, Alicia Juana
Paulazo, Maria Alejandra
Díaz Flaqué, María Celeste
Franchi, A. M.
Cremaschi, Graciela A.
author_role author
author2 Sterle, Helena Andrea
Vercelli, C.
Valli, Eduardo
Cayrol, María Florencia
Klecha, Alicia Juana
Paulazo, Maria Alejandra
Díaz Flaqué, María Celeste
Franchi, A. M.
Cremaschi, Graciela A.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
topic APOPTOSIS
ADN
GENES
MITOCONDRIA
NITRATOS
PROTEINAS
TIEMPO
LINFOMA DE CELULAS T
dc.description.none.fl_txt_mv Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Vercelli, C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biomedicina; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klecha, Alicia Juana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Paulazo, Maria Alejandra. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Flaqué, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Franchi, A. M. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Franchi, A. M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Cremaschi, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Abstract: Thyroid hormones are important regulators of cell physiology, inducing cell proliferation, differentiation or apoptosis, depending on the cell type. Thyroid hormones induce proliferation in short-term T lymphocyte cultures. In this study, we assessed the effect of long-term thyroxine (T4) treatment on the balance of proliferation and apoptosis and the intermediate participants in T lymphoma cells. Treatment with T4 affected this balance from the fifth day of culture, inhibiting proliferation in a time-dependent manner. This effect was associated with apoptosis induction, as characterized through nuclear morphological changes, DNA fragmentation, and Annexin V-FITC/Propidium Iodide co-staining. In addition, increased iNOS gene and protein levels, and enzyme activity were observed. The generation of reactive oxygen species, depolarization of the mitochondrial membrane, and a reduction in glutathione levels were also observed. The imbalance between oxidants and antioxidants species is typically associated with the nitration of proteins, including PKCζ, an isoenzyme essential for lymphoma cell division and survival. Consistently, evidence of PKCζ nitration via proteasome degradation was also observed in this study. Taken together, these results suggest that the long-term culture of T lymphoma cells with T4 induces apoptosis through the increased production of oxidative species resulting from both augmented iNOS activity and the loss of mitochondrial function. These species induce the nitration of proteins involved in cell viability, promoting proteasome degradation. Furthermore, we discuss the impact of these results on the modulation of T lymphoma growth and the thyroid status in vivo.
description Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/15265
1573-675X (online)
1360-8185
10.1007/s10495-013-0869-8
23733107
Barreiro Arcos, M.L. Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase [en línea]. Apoptosis. 2013. 18(11) doi:10.1007/s10495-013-0869-8 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15265
url https://repositorio.uca.edu.ar/handle/123456789/15265
identifier_str_mv 1573-675X (online)
1360-8185
10.1007/s10495-013-0869-8
23733107
Barreiro Arcos, M.L. Induction of apoptosis in T lymphoma cells by long-term treatment with thyroxine involves PKCf nitration by nitric oxide synthase [en línea]. Apoptosis. 2013. 18(11) doi:10.1007/s10495-013-0869-8 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15265
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Apoptosis. 2013. 18(11)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.070432

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