How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?

Autores
Santos, María Victoria; Sansiñena, Marina Julia; Zaritzky, Noemí; Chirife, Jorge
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Faculta de Ingeniería y Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chirife, Jorge. Pontificia Universidad Católica Argentina. Faculta de Ingeniería y Ciencias Agrarias; Argentina
Abstract: The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender packaged in straw was extended to predict internal temperature gradients during the cooling/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen/extender “liquid” column was 1.5 ºC for an external heat transfer coefficient, h = 15 W/(m2 K), and only 0.5 ºC for h = 5 W/(m2 K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoa
Fuente
CryoLetters, 34(2), 2013
Materia
CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/5491
Acceder
id RIUCA_ddb3147cb2dbc5eb93dbd9b671b61759
oai_identifier_str oai:ucacris:123456789/5491
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?Santos, María VictoriaSansiñena, Marina JuliaZaritzky, NoemíChirife, JorgeCONGELACIONESPERMA BOVINOTRANSFERENCIA DE CALORVAPOR DE NITROGENOCOEFICIENTEFil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; ArgentinaFil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Faculta de Ingeniería y Ciencias Agrarias; ArgentinaFil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; ArgentinaFil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chirife, Jorge. Pontificia Universidad Católica Argentina. Faculta de Ingeniería y Ciencias Agrarias; ArgentinaAbstract: The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender packaged in straw was extended to predict internal temperature gradients during the cooling/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen/extender “liquid” column was 1.5 ºC for an external heat transfer coefficient, h = 15 W/(m2 K), and only 0.5 ºC for h = 5 W/(m2 K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoaIngenta2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/54910143-20441742-0644 (Online)Santos, M. V., Sansinena, M., Zaritzky, N. y J. Chirife. 2013. How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor? [en línea]. CryoLetters 34 (2). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5491CryoLetters, 34(2), 2013reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:07Zoai:ucacris:123456789/5491instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:07.964Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
spellingShingle How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
Santos, María Victoria
CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
title_short How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_full How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_fullStr How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_full_unstemmed How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
title_sort How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
dc.creator.none.fl_str_mv Santos, María Victoria
Sansiñena, Marina Julia
Zaritzky, Noemí
Chirife, Jorge
author Santos, María Victoria
author_facet Santos, María Victoria
Sansiñena, Marina Julia
Zaritzky, Noemí
Chirife, Jorge
author_role author
author2 Sansiñena, Marina Julia
Zaritzky, Noemí
Chirife, Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
topic CONGELACION
ESPERMA BOVINO
TRANSFERENCIA DE CALOR
VAPOR DE NITROGENO
COEFICIENTE
dc.description.none.fl_txt_mv Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Faculta de Ingeniería y Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chirife, Jorge. Pontificia Universidad Católica Argentina. Faculta de Ingeniería y Ciencias Agrarias; Argentina
Abstract: The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender packaged in straw was extended to predict internal temperature gradients during the cooling/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen/extender “liquid” column was 1.5 ºC for an external heat transfer coefficient, h = 15 W/(m2 K), and only 0.5 ºC for h = 5 W/(m2 K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoa
description Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingenierías. Departamento de Ingeniería; Argentina
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/5491
0143-2044
1742-0644 (Online)
Santos, M. V., Sansinena, M., Zaritzky, N. y J. Chirife. 2013. How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor? [en línea]. CryoLetters 34 (2). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5491
url https://repositorio.uca.edu.ar/handle/123456789/5491
identifier_str_mv 0143-2044
1742-0644 (Online)
Santos, M. V., Sansinena, M., Zaritzky, N. y J. Chirife. 2013. How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor? [en línea]. CryoLetters 34 (2). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5491
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Ingenta
publisher.none.fl_str_mv Ingenta
dc.source.none.fl_str_mv CryoLetters, 34(2), 2013
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.070432

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