Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen

Autores
Santos, Maria Victoria; Sansinena, Marina Julia; Zaritzky, Noemi Elisabet; Chirife, Jorge
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A widespread practice in cryopreservation is to freeze spermatozoa by suspending the straws in stagnant nitrogen vapor over liquid nitrogen (N2V/LN2) for variable periods of time before plunging into liquid nitrogen (-196 C) for indefinite storage. A mathematical heat transfer model was developed to predict freezing times (phase change was considered) required for bull semen and extender packaged in 0.5 ml plastic straws and suspended in static liquid nitrogen vapor. Thermophysical properties (i.e. thermal conductivity, specific heat, density, initial freezing temperature) of bovine semen and extender as a function of temperature were determined considering the water change of phase. The non-stationary heat transfer partial differential equations with variable properties (nonlinear mathematical problem) were numerically solved considering in series thermal resistances (semen suspension?straw) and the temperature profiles were obtained for both semen suspension and plastic straw. It was observed both the external heat transfer coefficient in stagnant nitrogen vapor and its temperature (controlled by the distance from the surface of liquid nitrogen to the straw) affected freezing times. The accuracy of the model to estimate freezing times of the straws was further confirmed by comparing with experimental literature data. Results of this study will be useful to select ??safe?? holding times of bull semen in plastic straws placed N2V/LN2 to ensure that complete freezing of the sample has occurred in the nitrogen vapor and avoid cryodamage when plunging in LN2. Freezing times predicted by the numerical model can be applied to optimize freezing protocols of bull semen in straws.
Fil: Santos, Maria Victoria. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Fil: Sansinena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemi Elisabet. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Chirife, Jorge. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Agrarias; Argentina
Materia
FREEZING
BOVINE SPERMATOZOA
HEAT TRANSFER COEFFICIENT
COOLING RATE
NITROGEN VAPOR
LIQUID NITROGEN
NUMERICAL SIMULATION
FINITE ELEMENT METHOD
PLASTIC STRAWS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102209
Acceder
id CONICETDig_1a44418c4dfa70474545a2cd53bdb58d
oai_identifier_str oai:ri.conicet.gov.ar:11336/102209
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogenSantos, Maria VictoriaSansinena, Marina JuliaZaritzky, Noemi ElisabetChirife, JorgeFREEZINGBOVINE SPERMATOZOAHEAT TRANSFER COEFFICIENTCOOLING RATENITROGEN VAPORLIQUID NITROGENNUMERICAL SIMULATIONFINITE ELEMENT METHODPLASTIC STRAWShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2A widespread practice in cryopreservation is to freeze spermatozoa by suspending the straws in stagnant nitrogen vapor over liquid nitrogen (N2V/LN2) for variable periods of time before plunging into liquid nitrogen (-196 C) for indefinite storage. A mathematical heat transfer model was developed to predict freezing times (phase change was considered) required for bull semen and extender packaged in 0.5 ml plastic straws and suspended in static liquid nitrogen vapor. Thermophysical properties (i.e. thermal conductivity, specific heat, density, initial freezing temperature) of bovine semen and extender as a function of temperature were determined considering the water change of phase. The non-stationary heat transfer partial differential equations with variable properties (nonlinear mathematical problem) were numerically solved considering in series thermal resistances (semen suspension?straw) and the temperature profiles were obtained for both semen suspension and plastic straw. It was observed both the external heat transfer coefficient in stagnant nitrogen vapor and its temperature (controlled by the distance from the surface of liquid nitrogen to the straw) affected freezing times. The accuracy of the model to estimate freezing times of the straws was further confirmed by comparing with experimental literature data. Results of this study will be useful to select ??safe?? holding times of bull semen in plastic straws placed N2V/LN2 to ensure that complete freezing of the sample has occurred in the nitrogen vapor and avoid cryodamage when plunging in LN2. Freezing times predicted by the numerical model can be applied to optimize freezing protocols of bull semen in straws.Fil: Santos, Maria Victoria. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaFil: Sansinena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Agrarias; ArgentinaFil: Zaritzky, Noemi Elisabet. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Chirife, Jorge. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Agrarias; ArgentinaAcademic Press Inc Elsevier Science2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102209Santos, Maria Victoria; Sansinena, Marina Julia; Zaritzky, Noemi Elisabet; Chirife, Jorge; Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen; Academic Press Inc Elsevier Science; Cryobiology; 66; 1; 2-2013; 30-370011-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2012.10.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224012002337info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:13:36Zoai:ri.conicet.gov.ar:11336/102209instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:13:37.119CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
title Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
spellingShingle Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
Santos, Maria Victoria
FREEZING
BOVINE SPERMATOZOA
HEAT TRANSFER COEFFICIENT
COOLING RATE
NITROGEN VAPOR
LIQUID NITROGEN
NUMERICAL SIMULATION
FINITE ELEMENT METHOD
PLASTIC STRAWS
title_short Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
title_full Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
title_fullStr Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
title_full_unstemmed Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
title_sort Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen
dc.creator.none.fl_str_mv Santos, Maria Victoria
Sansinena, Marina Julia
Zaritzky, Noemi Elisabet
Chirife, Jorge
author Santos, Maria Victoria
author_facet Santos, Maria Victoria
Sansinena, Marina Julia
Zaritzky, Noemi Elisabet
Chirife, Jorge
author_role author
author2 Sansinena, Marina Julia
Zaritzky, Noemi Elisabet
Chirife, Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv FREEZING
BOVINE SPERMATOZOA
HEAT TRANSFER COEFFICIENT
COOLING RATE
NITROGEN VAPOR
LIQUID NITROGEN
NUMERICAL SIMULATION
FINITE ELEMENT METHOD
PLASTIC STRAWS
topic FREEZING
BOVINE SPERMATOZOA
HEAT TRANSFER COEFFICIENT
COOLING RATE
NITROGEN VAPOR
LIQUID NITROGEN
NUMERICAL SIMULATION
FINITE ELEMENT METHOD
PLASTIC STRAWS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A widespread practice in cryopreservation is to freeze spermatozoa by suspending the straws in stagnant nitrogen vapor over liquid nitrogen (N2V/LN2) for variable periods of time before plunging into liquid nitrogen (-196 C) for indefinite storage. A mathematical heat transfer model was developed to predict freezing times (phase change was considered) required for bull semen and extender packaged in 0.5 ml plastic straws and suspended in static liquid nitrogen vapor. Thermophysical properties (i.e. thermal conductivity, specific heat, density, initial freezing temperature) of bovine semen and extender as a function of temperature were determined considering the water change of phase. The non-stationary heat transfer partial differential equations with variable properties (nonlinear mathematical problem) were numerically solved considering in series thermal resistances (semen suspension?straw) and the temperature profiles were obtained for both semen suspension and plastic straw. It was observed both the external heat transfer coefficient in stagnant nitrogen vapor and its temperature (controlled by the distance from the surface of liquid nitrogen to the straw) affected freezing times. The accuracy of the model to estimate freezing times of the straws was further confirmed by comparing with experimental literature data. Results of this study will be useful to select ??safe?? holding times of bull semen in plastic straws placed N2V/LN2 to ensure that complete freezing of the sample has occurred in the nitrogen vapor and avoid cryodamage when plunging in LN2. Freezing times predicted by the numerical model can be applied to optimize freezing protocols of bull semen in straws.
Fil: Santos, Maria Victoria. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Fil: Sansinena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemi Elisabet. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Chirife, Jorge. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Agrarias; Argentina
description A widespread practice in cryopreservation is to freeze spermatozoa by suspending the straws in stagnant nitrogen vapor over liquid nitrogen (N2V/LN2) for variable periods of time before plunging into liquid nitrogen (-196 C) for indefinite storage. A mathematical heat transfer model was developed to predict freezing times (phase change was considered) required for bull semen and extender packaged in 0.5 ml plastic straws and suspended in static liquid nitrogen vapor. Thermophysical properties (i.e. thermal conductivity, specific heat, density, initial freezing temperature) of bovine semen and extender as a function of temperature were determined considering the water change of phase. The non-stationary heat transfer partial differential equations with variable properties (nonlinear mathematical problem) were numerically solved considering in series thermal resistances (semen suspension?straw) and the temperature profiles were obtained for both semen suspension and plastic straw. It was observed both the external heat transfer coefficient in stagnant nitrogen vapor and its temperature (controlled by the distance from the surface of liquid nitrogen to the straw) affected freezing times. The accuracy of the model to estimate freezing times of the straws was further confirmed by comparing with experimental literature data. Results of this study will be useful to select ??safe?? holding times of bull semen in plastic straws placed N2V/LN2 to ensure that complete freezing of the sample has occurred in the nitrogen vapor and avoid cryodamage when plunging in LN2. Freezing times predicted by the numerical model can be applied to optimize freezing protocols of bull semen in straws.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102209
Santos, Maria Victoria; Sansinena, Marina Julia; Zaritzky, Noemi Elisabet; Chirife, Jorge; Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen; Academic Press Inc Elsevier Science; Cryobiology; 66; 1; 2-2013; 30-37
0011-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102209
identifier_str_mv Santos, Maria Victoria; Sansinena, Marina Julia; Zaritzky, Noemi Elisabet; Chirife, Jorge; Mathematical prediction of freezing times of bovine semen straws placed in static vapor over liquid nitrogen; Academic Press Inc Elsevier Science; Cryobiology; 66; 1; 2-2013; 30-37
0011-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2012.10.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224012002337
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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