Salt stress on Lotus tenuis triggers cell wall polysaccharide changes affecting their digestibility by ruminants

• Autores: Vago, María Elena; Jaurena, Gustavo; Estevez, José M.; Agueda Castro, María; Zavala, Jorge Alberto; Ciancia, Marina
• Año de publicación: 2021
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plant Physiology and Biochemistry. 2021, 166
• Materia: RUMIANTE; LOTUS TENUIS; SALINIDAD; GANADERIA; NUTRICION ANIMAL; PARED CELULAR
• Institución: Pontificia Universidad Católica Argentina

Fil: Vago, María Elena. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Evaluación de Alimentos para Uso Animal; Argentina
Fil: Jaurena, Gustavo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Nutrición Animal; Argentina
Fil: Estevez, José M. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Estevez, José M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Estevez, José M. Universidad Andres Bello.Facultad de Ciencias de La Vida. Centro de Biotecnología Vegetal; Chile
Fil: Estevez, José M. Millennium Institute for Integrative Biology; Chile
Fil: Agueda Castro, María. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Laboratorio de Anatomía Vegetal Aplicada; Argentina
Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ciancia, Marina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Química de Biomoléculas; Argentina
Fil: Ciancia, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Lotus tenuis is a glycophytic forage legume (Fabaceae) used in feeding ruminants that can grow under salinity and waterlogging stresses. Plants obtained in controlled conditions were affected negatively in their growth by the effect of salt. Results from sequential extraction of plant cell wall polysaccharides and chemical characterization were related to those from nutritional parameters used to assess ruminants feedstuffs (Van Soest detergent system). Shoots and leaves were analyzed, and the most important differences were found for shoots. The salt-stressed shoots gave lower values of neutral detergent fiber and acid detergent fiber; they produced higher amounts of reserve α-glucans, and hemicelluloses (xyloglucans and glucuronoxylans from primary and secondary cell walls, respectively) and pectins, leaving less material resistant to extraction. This effect was clearly confirmed by an in vitro gas production assay. In addition, observations by light microcopy (LM) and transmission electron microscopy (TEM), showed in some tissues thicker walls and more opened cell wall structures in regard to control samples, which could allow easier access of degrading enzymes in the rumen. Although the plant biomass of Lotus tenuis produced under salt stress was lower, its quality as forage improved due to production of increased quantities of more digestible polysaccharides.