Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice

Autores
Cha, Hye Lim; Choi, Jung-Mi; Oh, Huy-Hyen; Bashyal, Narayan; Kim, Sung-Soo; Birnbaumer, Lutz; Suh-Kim, Haeyoung
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Cha, Hye Lim. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Choi, Jung-Mi. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Oh, Huy-Hyen. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Bashyal, Narayan. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Bashyal, Narayan. Ajou University School of Medicine. The Graduate School. Departments of Biomedical Sciences; South Korea
Fil: Kim, Sung-Soo. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Birnbaumer, Lutz. Pontificia Univerisdad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Birnbaumer, Lutz. Research Triangle Park. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos
Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South Korea
Abtract: Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.
Fuente
Molecular Brain. 2019;12(1):57
Materia
SISTEMA NERVIOSO CENTRAL
CEREBELO
PROTEINAS
HIPOPLASIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/8680
Acceder
id RIUCA_44aca01e90d9ac2714995642f338dc46
oai_identifier_str oai:ucacris:123456789/8680
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in miceCha, Hye LimChoi, Jung-MiOh, Huy-HyenBashyal, NarayanKim, Sung-SooBirnbaumer, LutzSuh-Kim, HaeyoungSISTEMA NERVIOSO CENTRALCEREBELOPROTEINASHIPOPLASIAFil: Cha, Hye Lim. Ajou University School of Medicine. Departments of Anatomy; South KoreaFil: Choi, Jung-Mi. Ajou University School of Medicine. Departments of Anatomy; South KoreaFil: Oh, Huy-Hyen. Ajou University School of Medicine. Departments of Anatomy; South KoreaFil: Bashyal, Narayan. Ajou University School of Medicine. Departments of Anatomy; South KoreaFil: Bashyal, Narayan. Ajou University School of Medicine. The Graduate School. Departments of Biomedical Sciences; South KoreaFil: Kim, Sung-Soo. Ajou University School of Medicine. Departments of Anatomy; South KoreaFil: Birnbaumer, Lutz. Pontificia Univerisdad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Birnbaumer, Lutz. Research Triangle Park. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados UnidosFil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South KoreaFil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South KoreaAbtract: Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.BMC2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/86801756-660610.1186/s13041-019-0477-931221179Cha HL, Choi J-M, Oh H-H, et al. Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice [en línea]. Molecular Brain. 2019;12(1):57. doi:10.1186/s13041-019-0477-9 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8680Molecular Brain. 2019;12(1):57reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:54Zoai:ucacris:123456789/8680instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:54.99Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
spellingShingle Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
Cha, Hye Lim
SISTEMA NERVIOSO CENTRAL
CEREBELO
PROTEINAS
HIPOPLASIA
title_short Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_full Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_fullStr Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_full_unstemmed Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_sort Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
dc.creator.none.fl_str_mv Cha, Hye Lim
Choi, Jung-Mi
Oh, Huy-Hyen
Bashyal, Narayan
Kim, Sung-Soo
Birnbaumer, Lutz
Suh-Kim, Haeyoung
author Cha, Hye Lim
author_facet Cha, Hye Lim
Choi, Jung-Mi
Oh, Huy-Hyen
Bashyal, Narayan
Kim, Sung-Soo
Birnbaumer, Lutz
Suh-Kim, Haeyoung
author_role author
author2 Choi, Jung-Mi
Oh, Huy-Hyen
Bashyal, Narayan
Kim, Sung-Soo
Birnbaumer, Lutz
Suh-Kim, Haeyoung
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SISTEMA NERVIOSO CENTRAL
CEREBELO
PROTEINAS
HIPOPLASIA
topic SISTEMA NERVIOSO CENTRAL
CEREBELO
PROTEINAS
HIPOPLASIA
dc.description.none.fl_txt_mv Fil: Cha, Hye Lim. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Choi, Jung-Mi. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Oh, Huy-Hyen. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Bashyal, Narayan. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Bashyal, Narayan. Ajou University School of Medicine. The Graduate School. Departments of Biomedical Sciences; South Korea
Fil: Kim, Sung-Soo. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Birnbaumer, Lutz. Pontificia Univerisdad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Birnbaumer, Lutz. Research Triangle Park. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos
Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South Korea
Fil: Suh-Kim, Haeyoung. Ajou University School of Medicine. Departments of Anatomy; South Korea
Abtract: Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.
description Fil: Cha, Hye Lim. Ajou University School of Medicine. Departments of Anatomy; South Korea
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/8680
1756-6606
10.1186/s13041-019-0477-9
31221179
Cha HL, Choi J-M, Oh H-H, et al. Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice [en línea]. Molecular Brain. 2019;12(1):57. doi:10.1186/s13041-019-0477-9 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8680
url https://repositorio.uca.edu.ar/handle/123456789/8680
identifier_str_mv 1756-6606
10.1186/s13041-019-0477-9
31221179
Cha HL, Choi J-M, Oh H-H, et al. Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice [en línea]. Molecular Brain. 2019;12(1):57. doi:10.1186/s13041-019-0477-9 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8680
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Molecular Brain. 2019;12(1):57
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.069144

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