Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice

Autores
Cha, Hye Lim; Choi, Jung Mi; Oh, Huy Hyen; Bashyal, Narayan; Kim, Sung-Soo; Birnbaumer, Lutz; Suh Kim, Haeyoung
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.
Fil: Cha, Hye Lim. Ajou University, School Of Medicine; Corea del Sur
Fil: Choi, Jung Mi. Ajou University, School Of Medicine; Corea del Sur
Fil: Oh, Huy Hyen. Ajou University, School Of Medicine; Corea del Sur
Fil: Bashyal, Narayan. Ajou University, School Of Medicine; Corea del Sur
Fil: Kim, Sung-Soo. Ajou University, School Of Medicine; Corea del Sur
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Suh Kim, Haeyoung. Ajou University, School Of Medicine; Corea del Sur
Materia
CEREBELLAR DEVELOPMENT
CEREBELLUM
CLIMBING FIBER
GO ALPHA SUBUNIT (GΑO
GTP-BINDING PROTEIN ALPHA SUBUNIT OF GO)
HYPOPLASIA
PURKINJE CELL
SYNAPTIC BOUTONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120833
Acceder
id CONICETDig_256cf9bca2e326e146685b6952b8c4f8
oai_identifier_str oai:ri.conicet.gov.ar:11336/120833
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in miceCha, Hye LimChoi, Jung MiOh, Huy HyenBashyal, NarayanKim, Sung-SooBirnbaumer, LutzSuh Kim, HaeyoungCEREBELLAR DEVELOPMENTCEREBELLUMCLIMBING FIBERGO ALPHA SUBUNIT (GΑOGTP-BINDING PROTEIN ALPHA SUBUNIT OF GO)HYPOPLASIAPURKINJE CELLSYNAPTIC BOUTONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.Fil: Cha, Hye Lim. Ajou University, School Of Medicine; Corea del SurFil: Choi, Jung Mi. Ajou University, School Of Medicine; Corea del SurFil: Oh, Huy Hyen. Ajou University, School Of Medicine; Corea del SurFil: Bashyal, Narayan. Ajou University, School Of Medicine; Corea del SurFil: Kim, Sung-Soo. Ajou University, School Of Medicine; Corea del SurFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Suh Kim, Haeyoung. Ajou University, School Of Medicine; Corea del SurBioMed Central2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120833Cha, Hye Lim; Choi, Jung Mi; Oh, Huy Hyen; Bashyal, Narayan; Kim, Sung-Soo; et al.; Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice; BioMed Central; Molecular Brain; 12; 1; 6-2019; 1-121756-6606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13041-019-0477-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:01Zoai:ri.conicet.gov.ar:11336/120833instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:02.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
spellingShingle Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
Cha, Hye Lim
CEREBELLAR DEVELOPMENT
CEREBELLUM
CLIMBING FIBER
GO ALPHA SUBUNIT (GΑO
GTP-BINDING PROTEIN ALPHA SUBUNIT OF GO)
HYPOPLASIA
PURKINJE CELL
SYNAPTIC BOUTONS
title_short Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_full Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_fullStr Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_full_unstemmed Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
title_sort Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice
dc.creator.none.fl_str_mv Cha, Hye Lim
Choi, Jung Mi
Oh, Huy Hyen
Bashyal, Narayan
Kim, Sung-Soo
Birnbaumer, Lutz
Suh Kim, Haeyoung
author Cha, Hye Lim
author_facet Cha, Hye Lim
Choi, Jung Mi
Oh, Huy Hyen
Bashyal, Narayan
Kim, Sung-Soo
Birnbaumer, Lutz
Suh Kim, Haeyoung
author_role author
author2 Choi, Jung Mi
Oh, Huy Hyen
Bashyal, Narayan
Kim, Sung-Soo
Birnbaumer, Lutz
Suh Kim, Haeyoung
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CEREBELLAR DEVELOPMENT
CEREBELLUM
CLIMBING FIBER
GO ALPHA SUBUNIT (GΑO
GTP-BINDING PROTEIN ALPHA SUBUNIT OF GO)
HYPOPLASIA
PURKINJE CELL
SYNAPTIC BOUTONS
topic CEREBELLAR DEVELOPMENT
CEREBELLUM
CLIMBING FIBER
GO ALPHA SUBUNIT (GΑO
GTP-BINDING PROTEIN ALPHA SUBUNIT OF GO)
HYPOPLASIA
PURKINJE CELL
SYNAPTIC BOUTONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.
Fil: Cha, Hye Lim. Ajou University, School Of Medicine; Corea del Sur
Fil: Choi, Jung Mi. Ajou University, School Of Medicine; Corea del Sur
Fil: Oh, Huy Hyen. Ajou University, School Of Medicine; Corea del Sur
Fil: Bashyal, Narayan. Ajou University, School Of Medicine; Corea del Sur
Fil: Kim, Sung-Soo. Ajou University, School Of Medicine; Corea del Sur
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Suh Kim, Haeyoung. Ajou University, School Of Medicine; Corea del Sur
description Go is a member of the pertussis toxin-sensitive Gi/o family. Despite its abundance in the central nervous system, the precise role of Go remains largely unknown compared to other G proteins. In the present study, we explored the functions of Go in the developing cerebellar cortex by deleting its gene, Gnao. We performed a histological analysis with cerebellar sections of adult mice by cresyl violet- and immunostaining. Global deletion of Gnao induced cerebellar hypoplasia, reduced arborization of Purkinje cell dendrites, and atrophied Purkinje cell dendritic spines and the terminal boutons of climbing fibers from the inferior olivary nucleus. These results indicate that Go-mediated signaling pathway regulates maturation of presynaptic parallel fibers from granule cells and climbing fibers during the cerebellar cortical development.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120833
Cha, Hye Lim; Choi, Jung Mi; Oh, Huy Hyen; Bashyal, Narayan; Kim, Sung-Soo; et al.; Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice; BioMed Central; Molecular Brain; 12; 1; 6-2019; 1-12
1756-6606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120833
identifier_str_mv Cha, Hye Lim; Choi, Jung Mi; Oh, Huy Hyen; Bashyal, Narayan; Kim, Sung-Soo; et al.; Deletion of the α subunit of the heterotrimeric Go protein impairs cerebellar cortical development in mice; BioMed Central; Molecular Brain; 12; 1; 6-2019; 1-12
1756-6606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13041-019-0477-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269319459766272
score 13.13397

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