Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology

• Autores: Elshafey, Naglaa; Selim, Samy; Mohammed, Asmaa H.; Hagagy, Nashwa; Samy, Mennatalla; Mostafa, Ehab M.; Safhi, Fatmah A.; Saddiq, Amna; Alsharari, Salam S.; Aseel, Dalia G.; Hafiz, Iram; Elkelish, Amr; Pérez, Leonardo Martín
• Año de publicación: 2022
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Fermentation. 2022, 8(8), 365
• Materia: HALOARQUEAS; BIOTECNOLOGIA; BIODIVERSIDAD
• Institución: Pontificia Universidad Católica Argentina

Fil: Elshafey, Naglaa. Al-Arish University. Faculty of Science. Botany and Microbiology Department; Egipto
Fil: Selim, Samy. Jouf University. College of Applied Medical Sciences. Department of Clinical Laboratory Sciences; Arabia Saudita
Fil: Mohammed, Asmaa H. National Authority for Remote Sensing and Space Sciences. Marine Sciences Department; Egipto
Fil: Hagagy, Nashwa. University of Jeddah. College of Science & Arts at Khulis. Department of Biology; Arabia Saudita
Fil: Hagagy, Nashwa. Suez Canal University. Faculty of Science. Botany and Microbiology Department; Egipto
Fil: Samy, Mennatalla. The Higher Institute of Engineering. Department of Communications and Computers Engineering; Egipto
Fil: Mostafa, Ehab M. Jouf University. College of Pharmacy. Department of Pharmacognosy. Arabia Saudita
Fil: Safhi, Fatmah A. Princess Nourah bint Abdulrahman University. College of Science. Department of Biology; Arabia Saudita
Fil: Salha M. Alshamrani. University of Jeddah. College of Science. Department of Biology; Arabia Saudita
Fil: Saddiq, Amna. University of Jeddah. College of Science & Arts at Khulis. Department of Biology; Arabia Saudita
Fil: Alsharari, Salam S. Jouf University. College of Science. Biology Department; Arabia Saudita
Fil: Aseel, Dalia G. City of Scientific Research and Technological Applications. Arid Lands Cultivation Research Institute. Plant Protection and Biomolecular Diagnosis Department; Egipto
Fil: Hafiz, Iram. University of Sargodha. Institute of Chemistry; Pakistán
Fil: Elkelish, Amr. Suez Canal University. Faculty of Science. Botany and Microbiology Department; Egipto
Fil: Pérez, Leonardo Martín. Pontificia Universidad Católica Argentina; Argentina
Fil: Pérez, Leonardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes, which was combined with remote sensing and geographic information system (GIS) data to highlight the distribution pattern of the microbial diversity in water and sediment samples. The majority of archaeal sequences identified in all four lakes belonged to the phyla Euryarchaeota and Crenarchaeota. Sediment samples from Beida Lake and water samples from El-Hamra Lake showed the highest levels of archaeal diversity. Sequence similarities ≥ 95% were found between six of the acquired clones and uncultured Halorhabdus, Euryarchaeota, and archaeon clones. In addition, two clones shared a high level of sequence similarity (97%) with unclassified archaea, while other nine clones exhibited 96% to 99% sequence similarity with uncultured archaeon clones, and only one clone showed 97% identity with an uncultured Crenarchaeota. Likewise, 7 DGGE bands presented a sequence similarity of 90 to 98% to Halogranum sp., Halalkalicoccus tibetensis, Halalkalicoccus jeotgali, uncultured Halorubrum, Halobacteriaceae sp., or uncultured haloarchaeon. In conclusion, while the variety of alkaliphilic haloarchaea in the examined soda lakes was restricted, the possibility of uncovering novel species for biotechnological applications from these extreme habitats remains promising.