Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse

Autores
Díaz, Gabriela Verónica; Zapata, Pedro Darío; Villalba, Laura Lidia; Fonseca, María Isabel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and b-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL1 of endoxylanases, respectively; 0.6 UmL1 of b-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.
Materia
Aspergillus
Endoxylanases
B-xylosidases
Proteases
Sugarcane bagasse
Multivariate analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Repositorio
Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
Institución
Universidad Nacional de Misiones
OAI Identificador
oai:rid.unam.edu.ar:20.500.12219/5428
Acceder
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network_acronym_str RIDUNaM
repository_id_str
network_name_str Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
spelling Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasseDíaz, Gabriela VerónicaZapata, Pedro DaríoVillalba, Laura LidiaFonseca, María IsabelAspergillusEndoxylanasesB-xylosidasesProteasesSugarcane bagasseMultivariate analysisFil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Zapata, Pedro Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Villalba, Laura Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Fil: Fonseca, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and b-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL1 of endoxylanases, respectively; 0.6 UmL1 of b-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.Taylor & Francis2019-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf1.8 MBhttps://hdl.handle.net/20.500.12219/5428enginfo:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/107324info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1080/25765299.2019.1622922info:eu-repo/semantics/altIdentifier/urn/https://www.tandfonline.com/doi/epdf/10.1080/25765299.2019.1622922?needAccess=trueinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)instname:Universidad Nacional de Misiones2025-10-23T11:20:13Zoai:rid.unam.edu.ar:20.500.12219/5428instacron:UNAMInstitucionalhttps://rid.unam.edu.ar/Universidad públicahttps://www.unam.edu.ar/https://rid.unam.edu.ar/oai/rsnrdArgentinaopendoar:2025-10-23 11:20:13.725Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misionesfalse
dc.title.none.fl_str_mv Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
spellingShingle Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
Díaz, Gabriela Verónica
Aspergillus
Endoxylanases
B-xylosidases
Proteases
Sugarcane bagasse
Multivariate analysis
title_short Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_full Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_fullStr Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_full_unstemmed Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_sort Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
dc.creator.none.fl_str_mv Díaz, Gabriela Verónica
Zapata, Pedro Darío
Villalba, Laura Lidia
Fonseca, María Isabel
author Díaz, Gabriela Verónica
author_facet Díaz, Gabriela Verónica
Zapata, Pedro Darío
Villalba, Laura Lidia
Fonseca, María Isabel
author_role author
author2 Zapata, Pedro Darío
Villalba, Laura Lidia
Fonseca, María Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv Aspergillus
Endoxylanases
B-xylosidases
Proteases
Sugarcane bagasse
Multivariate analysis
topic Aspergillus
Endoxylanases
B-xylosidases
Proteases
Sugarcane bagasse
Multivariate analysis
dc.description.none.fl_txt_mv Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and b-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL1 of endoxylanases, respectively; 0.6 UmL1 of b-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.
description Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12219/5428
url https://hdl.handle.net/20.500.12219/5428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/107324
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1080/25765299.2019.1622922
info:eu-repo/semantics/altIdentifier/urn/https://www.tandfonline.com/doi/epdf/10.1080/25765299.2019.1622922?needAccess=true
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
application/pdf
1.8 MB
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
instname:Universidad Nacional de Misiones
reponame_str Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
collection Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
instname_str Universidad Nacional de Misiones
repository.name.fl_str_mv Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misiones
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