Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse

Autores
Díaz, Gabriela Verónica; Zapata, Pedro Dario; Villalba, Laura; Fonseca, Maria Isabel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and β-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL−1 of endoxylanases, respectively; 0.6 UmL−1 of β-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Villalba, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia
ASPERGILLUS
ENDOXYLANASES
B-XYLOSIDASES
PROTEASES
SUGARCANE BAGASSE
MULTIVARIATE ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107324
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/107324
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasseDíaz, Gabriela VerónicaZapata, Pedro DarioVillalba, LauraFonseca, Maria IsabelASPERGILLUSENDOXYLANASESB-XYLOSIDASESPROTEASESSUGARCANE BAGASSEMULTIVARIATE ANALYSIShttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and β-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL−1 of endoxylanases, respectively; 0.6 UmL−1 of β-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Villalba, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaTaylor & Francis2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107324Díaz, Gabriela Verónica; Zapata, Pedro Dario; Villalba, Laura; Fonseca, Maria Isabel; Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse; Taylor & Francis; Arab Journal of Basic and Applied Sciences; 26; 1; 7-2019; 292-3012576-5299CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1622922info:eu-repo/semantics/altIdentifier/doi/10.1080/25765299.2019.1622922info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:34Zoai:ri.conicet.gov.ar:11336/107324instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:34.306CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
spellingShingle Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
Díaz, Gabriela Verónica
ASPERGILLUS
ENDOXYLANASES
B-XYLOSIDASES
PROTEASES
SUGARCANE BAGASSE
MULTIVARIATE ANALYSIS
title_short Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_full Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_fullStr Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_full_unstemmed Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
title_sort Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
dc.creator.none.fl_str_mv Díaz, Gabriela Verónica
Zapata, Pedro Dario
Villalba, Laura
Fonseca, Maria Isabel
author Díaz, Gabriela Verónica
author_facet Díaz, Gabriela Verónica
Zapata, Pedro Dario
Villalba, Laura
Fonseca, Maria Isabel
author_role author
author2 Zapata, Pedro Dario
Villalba, Laura
Fonseca, Maria Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv ASPERGILLUS
ENDOXYLANASES
B-XYLOSIDASES
PROTEASES
SUGARCANE BAGASSE
MULTIVARIATE ANALYSIS
topic ASPERGILLUS
ENDOXYLANASES
B-XYLOSIDASES
PROTEASES
SUGARCANE BAGASSE
MULTIVARIATE ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and β-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL−1 of endoxylanases, respectively; 0.6 UmL−1 of β-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Villalba, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and β-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL−1 of endoxylanases, respectively; 0.6 UmL−1 of β-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107324
Díaz, Gabriela Verónica; Zapata, Pedro Dario; Villalba, Laura; Fonseca, Maria Isabel; Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse; Taylor & Francis; Arab Journal of Basic and Applied Sciences; 26; 1; 7-2019; 292-301
2576-5299
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107324
identifier_str_mv Díaz, Gabriela Verónica; Zapata, Pedro Dario; Villalba, Laura; Fonseca, Maria Isabel; Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse; Taylor & Francis; Arab Journal of Basic and Applied Sciences; 26; 1; 7-2019; 292-301
2576-5299
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1622922
info:eu-repo/semantics/altIdentifier/doi/10.1080/25765299.2019.1622922
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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