Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediat...
- Autores
- Conde Molina, Débora; Liporace, Franco; Quevedo, Carla
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Contaminated sites with petroleum compounds are frequently observed, requiring the development of innovative technologies for its remediation. The problem is caused due to the widespread usage of petroleum-based products. Their discharge and accidental spillage in the environment prove to be hazardous both to the surroundings and life forms. Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. It is a non-invasive and cost-effective technique that relies on natural decontamination using microbes of isolated strains from contaminated areas for the clean-up of these petroleum hydrocarbons. The Zárate-Campana industrial center, located in Buenos Aires, represents one of the most important petrochemical areas in Argentina, with several companies carrying out petrochemical activities. In this study, we have investigated the ability of microorganisms to degrade these hydrocarbons. Samples were collected in the surroundings of the Campana area and screened for hydrocarbon degrading bacteria. 4 of the 13 strains previously isolated from contaminated sites were screened and identified as Pseudomonas sp, Cellulosimicrobium sp and Ochrobactrum sp. A new approach using MT1A3, belonging to Pseudomonas genus in petroleum biodegradation from the use of different carbon and nitrogen sources, was proposed to provide maximum biomass production and was evaluated for its degradation characteristics. MT1A3 grew in all carbon sources tested and was able to grow in a hydrocarbon mixture obtaining 1.79 g/L of biomass production at 25 ºC after 7 days. When comparing the use of different low-cost agro-industrial co-products as an alternative carbon source, the biomass production was significantly higher in crude peanut oil in comparison to all other substrates (p < 0.05), thus resulting in a biomass of 7.29 g/L. The most efficient nitrogen source for obtaining biomass from MT1A3 was NaNO3. Based on these results, the effectiveness was evaluated by monitoring total hydrocarbons (THs) and n-alkanes degradation as well as changes in bacterial population of natural attenuation, biostimulation and bioaugmentation treatments in microcosm design over a 120-day period. The best treatment, which involved bioaugmentation (MT1A3) and biostimulation strategies, showed a degradation of 40.05 % of total hydrocarbons with respect to the natural attenuation treatment used as control. The highest concentration of THAB and HDB was recorded, reaching a value of 2,17x1010 CFU and 8,91x106 UFC respectively.
Fil: Universidad Tecnológica Nacional. Facultad Regional Delta
Peer Reviewed - Materia
-
UTN
FRD
Biorremediación
Suelos contaminados
Hidrocarburos
Microcosmos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Universidad Tecnológica Nacional
- OAI Identificador
- oai:ria.utn.edu.ar:20.500.12272/3926
Ver los metadatos del registro completo
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Repositorio Institucional Abierto (UTN) |
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Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies.Conde Molina, DéboraLiporace, FrancoQuevedo, CarlaUTNFRDBiorremediaciónSuelos contaminadosHidrocarburosMicrocosmosContaminated sites with petroleum compounds are frequently observed, requiring the development of innovative technologies for its remediation. The problem is caused due to the widespread usage of petroleum-based products. Their discharge and accidental spillage in the environment prove to be hazardous both to the surroundings and life forms. Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. It is a non-invasive and cost-effective technique that relies on natural decontamination using microbes of isolated strains from contaminated areas for the clean-up of these petroleum hydrocarbons. The Zárate-Campana industrial center, located in Buenos Aires, represents one of the most important petrochemical areas in Argentina, with several companies carrying out petrochemical activities. In this study, we have investigated the ability of microorganisms to degrade these hydrocarbons. Samples were collected in the surroundings of the Campana area and screened for hydrocarbon degrading bacteria. 4 of the 13 strains previously isolated from contaminated sites were screened and identified as Pseudomonas sp, Cellulosimicrobium sp and Ochrobactrum sp. A new approach using MT1A3, belonging to Pseudomonas genus in petroleum biodegradation from the use of different carbon and nitrogen sources, was proposed to provide maximum biomass production and was evaluated for its degradation characteristics. MT1A3 grew in all carbon sources tested and was able to grow in a hydrocarbon mixture obtaining 1.79 g/L of biomass production at 25 ºC after 7 days. When comparing the use of different low-cost agro-industrial co-products as an alternative carbon source, the biomass production was significantly higher in crude peanut oil in comparison to all other substrates (p < 0.05), thus resulting in a biomass of 7.29 g/L. The most efficient nitrogen source for obtaining biomass from MT1A3 was NaNO3. Based on these results, the effectiveness was evaluated by monitoring total hydrocarbons (THs) and n-alkanes degradation as well as changes in bacterial population of natural attenuation, biostimulation and bioaugmentation treatments in microcosm design over a 120-day period. The best treatment, which involved bioaugmentation (MT1A3) and biostimulation strategies, showed a degradation of 40.05 % of total hydrocarbons with respect to the natural attenuation treatment used as control. The highest concentration of THAB and HDB was recorded, reaching a value of 2,17x1010 CFU and 8,91x106 UFC respectively.Fil: Universidad Tecnológica Nacional. Facultad Regional DeltaPeer Reviewed2019-08-26T23:36:09Z2019-08-26T23:36:09Z2019-07-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf2525-8761http://hdl.handle.net/20.500.12272/392610.34117engenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/El autorAtribución (BY)Attribution-NonCommercial-NoDerivatives 4.0 Internacionalreponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2025-09-04T11:14:41Zoai:ria.utn.edu.ar:20.500.12272/3926instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:14:41.59Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse |
dc.title.none.fl_str_mv |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
title |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
spellingShingle |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. Conde Molina, Débora UTN FRD Biorremediación Suelos contaminados Hidrocarburos Microcosmos |
title_short |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
title_full |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
title_fullStr |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
title_full_unstemmed |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
title_sort |
Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies. |
dc.creator.none.fl_str_mv |
Conde Molina, Débora Liporace, Franco Quevedo, Carla |
author |
Conde Molina, Débora |
author_facet |
Conde Molina, Débora Liporace, Franco Quevedo, Carla |
author_role |
author |
author2 |
Liporace, Franco Quevedo, Carla |
author2_role |
author author |
dc.subject.none.fl_str_mv |
UTN FRD Biorremediación Suelos contaminados Hidrocarburos Microcosmos |
topic |
UTN FRD Biorremediación Suelos contaminados Hidrocarburos Microcosmos |
dc.description.none.fl_txt_mv |
Contaminated sites with petroleum compounds are frequently observed, requiring the development of innovative technologies for its remediation. The problem is caused due to the widespread usage of petroleum-based products. Their discharge and accidental spillage in the environment prove to be hazardous both to the surroundings and life forms. Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. It is a non-invasive and cost-effective technique that relies on natural decontamination using microbes of isolated strains from contaminated areas for the clean-up of these petroleum hydrocarbons. The Zárate-Campana industrial center, located in Buenos Aires, represents one of the most important petrochemical areas in Argentina, with several companies carrying out petrochemical activities. In this study, we have investigated the ability of microorganisms to degrade these hydrocarbons. Samples were collected in the surroundings of the Campana area and screened for hydrocarbon degrading bacteria. 4 of the 13 strains previously isolated from contaminated sites were screened and identified as Pseudomonas sp, Cellulosimicrobium sp and Ochrobactrum sp. A new approach using MT1A3, belonging to Pseudomonas genus in petroleum biodegradation from the use of different carbon and nitrogen sources, was proposed to provide maximum biomass production and was evaluated for its degradation characteristics. MT1A3 grew in all carbon sources tested and was able to grow in a hydrocarbon mixture obtaining 1.79 g/L of biomass production at 25 ºC after 7 days. When comparing the use of different low-cost agro-industrial co-products as an alternative carbon source, the biomass production was significantly higher in crude peanut oil in comparison to all other substrates (p < 0.05), thus resulting in a biomass of 7.29 g/L. The most efficient nitrogen source for obtaining biomass from MT1A3 was NaNO3. Based on these results, the effectiveness was evaluated by monitoring total hydrocarbons (THs) and n-alkanes degradation as well as changes in bacterial population of natural attenuation, biostimulation and bioaugmentation treatments in microcosm design over a 120-day period. The best treatment, which involved bioaugmentation (MT1A3) and biostimulation strategies, showed a degradation of 40.05 % of total hydrocarbons with respect to the natural attenuation treatment used as control. The highest concentration of THAB and HDB was recorded, reaching a value of 2,17x1010 CFU and 8,91x106 UFC respectively. Fil: Universidad Tecnológica Nacional. Facultad Regional Delta Peer Reviewed |
description |
Contaminated sites with petroleum compounds are frequently observed, requiring the development of innovative technologies for its remediation. The problem is caused due to the widespread usage of petroleum-based products. Their discharge and accidental spillage in the environment prove to be hazardous both to the surroundings and life forms. Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. It is a non-invasive and cost-effective technique that relies on natural decontamination using microbes of isolated strains from contaminated areas for the clean-up of these petroleum hydrocarbons. The Zárate-Campana industrial center, located in Buenos Aires, represents one of the most important petrochemical areas in Argentina, with several companies carrying out petrochemical activities. In this study, we have investigated the ability of microorganisms to degrade these hydrocarbons. Samples were collected in the surroundings of the Campana area and screened for hydrocarbon degrading bacteria. 4 of the 13 strains previously isolated from contaminated sites were screened and identified as Pseudomonas sp, Cellulosimicrobium sp and Ochrobactrum sp. A new approach using MT1A3, belonging to Pseudomonas genus in petroleum biodegradation from the use of different carbon and nitrogen sources, was proposed to provide maximum biomass production and was evaluated for its degradation characteristics. MT1A3 grew in all carbon sources tested and was able to grow in a hydrocarbon mixture obtaining 1.79 g/L of biomass production at 25 ºC after 7 days. When comparing the use of different low-cost agro-industrial co-products as an alternative carbon source, the biomass production was significantly higher in crude peanut oil in comparison to all other substrates (p < 0.05), thus resulting in a biomass of 7.29 g/L. The most efficient nitrogen source for obtaining biomass from MT1A3 was NaNO3. Based on these results, the effectiveness was evaluated by monitoring total hydrocarbons (THs) and n-alkanes degradation as well as changes in bacterial population of natural attenuation, biostimulation and bioaugmentation treatments in microcosm design over a 120-day period. The best treatment, which involved bioaugmentation (MT1A3) and biostimulation strategies, showed a degradation of 40.05 % of total hydrocarbons with respect to the natural attenuation treatment used as control. The highest concentration of THAB and HDB was recorded, reaching a value of 2,17x1010 CFU and 8,91x106 UFC respectively. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-26T23:36:09Z 2019-08-26T23:36:09Z 2019-07-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
2525-8761 http://hdl.handle.net/20.500.12272/3926 10.34117 |
identifier_str_mv |
2525-8761 10.34117 |
url |
http://hdl.handle.net/20.500.12272/3926 |
dc.language.none.fl_str_mv |
eng eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ El autor Atribución (BY) Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ El autor Atribución (BY) Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositorio Institucional Abierto (UTN) instname:Universidad Tecnológica Nacional |
reponame_str |
Repositorio Institucional Abierto (UTN) |
collection |
Repositorio Institucional Abierto (UTN) |
instname_str |
Universidad Tecnológica Nacional |
repository.name.fl_str_mv |
Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional |
repository.mail.fl_str_mv |
gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar |
_version_ |
1842344357259116544 |
score |
12.623145 |