Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris
- Autores
- Leavitt, William D.; Waldbauer, Jacob; Venceslau, Sofia S.; Sim, Min Sub; Zhang, Lichun; Boidi, Flavia Jaquelina; Plummer, Sydney; Díaz, Julia M.; Pereira, Inês A. C.; Bradley, Alexander S.
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America.
Fil: Leavitt, William D. Washington University in St. Louis. Department of Earth and Planetary Sciences, Missouri; United States of America.
Fil: Waldbauer, Jacob. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.
Fil: Venceslau, Sofia S. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.
Fil: Sim, Min Sub. Seoul National University. School of Earth and Environmental Sciences; South Korea.
Fil: Zhang, Lichun. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.
Fil: Flavia Jaquelina Boidi. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.
Fil: Flavia Jaquelina Boidi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina.
Fil: Flavia Jaquelina Boidi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Plummer, Sydney. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.
Fil: Díaz, Julia M. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.
Fil: Pereira, Inês A. C. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.
Fil: Bradley, Alexander S. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.
Fil: Bradley, Alexander S. Washington University in St. Louis. Division of Biology and Biomedical Sciences, Missouri; United States of America.
Abstract: Microbial sulfate reduction is central to the global carbon cycle and the redox evolu-tion of Earth's surface. Tracking the activity of sulfate reducing microorganisms overspace and time relies on a nuanced understanding of stable sulfur isotope fractiona-tion in the context of the biochemical machinery of the metabolism. Here, we link themagnitude of stable sulfur isotopic fractionation to proteomic and metabolite profilesunder different cellular energetic regimes. When energy availability is limited, cell-specific sulfate respiration rates and net sulfur isotope fractionation inversely covary. Beyond net S isotope fractionation values, we also quantified shifts in protein expres-sion, abundances and isotopic composition of intracellular S metabolites, and lipidstructures and lipid/water H isotope fractionation values. These coupled approachesreveal which protein abundances shift directly as a function of energy flux, those thatvary minimally, and those that may vary independent of energy flux and likely do notcontribute to shifts in S-isotope fractionation. By coupling the bulk S-isotope obser-vations with quantitative proteomics, we provide novel constraints for metabolic iso-tope models. Together, these results lay the foundation for more predictive metabolicfractionation models, alongside interpretations of environmental sulfur and sulfatereducer lipid-H isotope data.
info:eu-repo/semantics/publishedVersion
Fil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America.
Fil: Leavitt, William D. Washington University in St. Louis. Department of Earth and Planetary Sciences, Missouri; United States of America.
Fil: Waldbauer, Jacob. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.
Fil: Venceslau, Sofia S. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.
Fil: Sim, Min Sub. Seoul National University. School of Earth and Environmental Sciences; South Korea.
Fil: Zhang, Lichun. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.
Fil: Flavia Jaquelina Boidi. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.
Fil: Flavia Jaquelina Boidi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina.
Fil: Flavia Jaquelina Boidi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Plummer, Sydney. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.
Fil: Díaz, Julia M. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.
Fil: Pereira, Inês A. C. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.
Fil: Bradley, Alexander S. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.
Fil: Bradley, Alexander S. Washington University in St. Louis. Division of Biology and Biomedical Sciences, Missouri; United States of America. - Materia
-
Chemostat
Compound-specific hydrogen isotopes
Microbial sulfate reduction
Quantitative proteomics
Stable sulfur isotopes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Universidad Nacional de Córdoba
- OAI Identificador
- oai:rdu.unc.edu.ar:11086/553191
Ver los metadatos del registro completo
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Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgarisLeavitt, William D.Waldbauer, JacobVenceslau, Sofia S.Sim, Min SubZhang, LichunBoidi, Flavia JaquelinaPlummer, SydneyDíaz, Julia M.Pereira, Inês A. C.Bradley, Alexander S.ChemostatCompound-specific hydrogen isotopesMicrobial sulfate reductionQuantitative proteomicsStable sulfur isotopesFil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America.Fil: Leavitt, William D. Washington University in St. Louis. Department of Earth and Planetary Sciences, Missouri; United States of America.Fil: Waldbauer, Jacob. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.Fil: Venceslau, Sofia S. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.Fil: Sim, Min Sub. Seoul National University. School of Earth and Environmental Sciences; South Korea.Fil: Zhang, Lichun. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.Fil: Flavia Jaquelina Boidi. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.Fil: Flavia Jaquelina Boidi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina.Fil: Flavia Jaquelina Boidi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Plummer, Sydney. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.Fil: Díaz, Julia M. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.Fil: Pereira, Inês A. C. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.Fil: Bradley, Alexander S. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.Fil: Bradley, Alexander S. Washington University in St. Louis. Division of Biology and Biomedical Sciences, Missouri; United States of America.Abstract: Microbial sulfate reduction is central to the global carbon cycle and the redox evolu-tion of Earth's surface. Tracking the activity of sulfate reducing microorganisms overspace and time relies on a nuanced understanding of stable sulfur isotope fractiona-tion in the context of the biochemical machinery of the metabolism. Here, we link themagnitude of stable sulfur isotopic fractionation to proteomic and metabolite profilesunder different cellular energetic regimes. When energy availability is limited, cell-specific sulfate respiration rates and net sulfur isotope fractionation inversely covary. Beyond net S isotope fractionation values, we also quantified shifts in protein expres-sion, abundances and isotopic composition of intracellular S metabolites, and lipidstructures and lipid/water H isotope fractionation values. These coupled approachesreveal which protein abundances shift directly as a function of energy flux, those thatvary minimally, and those that may vary independent of energy flux and likely do notcontribute to shifts in S-isotope fractionation. By coupling the bulk S-isotope obser-vations with quantitative proteomics, we provide novel constraints for metabolic iso-tope models. Together, these results lay the foundation for more predictive metabolicfractionation models, alongside interpretations of environmental sulfur and sulfatereducer lipid-H isotope data.info:eu-repo/semantics/publishedVersionFil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America.Fil: Leavitt, William D. Washington University in St. Louis. Department of Earth and Planetary Sciences, Missouri; United States of America.Fil: Waldbauer, Jacob. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.Fil: Venceslau, Sofia S. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.Fil: Sim, Min Sub. Seoul National University. School of Earth and Environmental Sciences; South Korea.Fil: Zhang, Lichun. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America.Fil: Flavia Jaquelina Boidi. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.Fil: Flavia Jaquelina Boidi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina.Fil: Flavia Jaquelina Boidi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Plummer, Sydney. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.Fil: Díaz, Julia M. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America.Fil: Pereira, Inês A. C. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal.Fil: Bradley, Alexander S. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America.Fil: Bradley, Alexander S. Washington University in St. Louis. Division of Biology and Biomedical Sciences, Missouri; United States of America.https://orcid.org/0000-0002-7909-2475https://orcid.org/0000-0002-0338-6143https://orcid.org/0000-0001-8774-1351https://orcid.org/0000-0002-3491-9002https://orcid.org/0000-0001-7135-87452024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfLeavitt, W. D., Waldbauer, J.,Venceslau, S. S., Sim, M. S., Zhang, L., Boidi, F. J., Plummer, S.,Diaz, J. M., Pereira, I. A. C., & Bradley, A. S. (2024). Energyflux couples sulfur isotope fractionation to proteomic andmetabolite profiles in Desulfovibrio vulgaris. Geobiology, 22,e12600. https://doi.org/10.1111/gbi.126001472-4677http://hdl.handle.net/11086/5531911472-4669https://doi.org/10.1111/gbi.12600enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:44:22Zoai:rdu.unc.edu.ar:11086/553191Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:44:22.593Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse |
| dc.title.none.fl_str_mv |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| title |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| spellingShingle |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris Leavitt, William D. Chemostat Compound-specific hydrogen isotopes Microbial sulfate reduction Quantitative proteomics Stable sulfur isotopes |
| title_short |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| title_full |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| title_fullStr |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| title_full_unstemmed |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| title_sort |
Energy flux couples sulfur isotope fractionation to proteomicand metabolite profiles in Desulfovibrio vulgaris |
| dc.creator.none.fl_str_mv |
Leavitt, William D. Waldbauer, Jacob Venceslau, Sofia S. Sim, Min Sub Zhang, Lichun Boidi, Flavia Jaquelina Plummer, Sydney Díaz, Julia M. Pereira, Inês A. C. Bradley, Alexander S. |
| author |
Leavitt, William D. |
| author_facet |
Leavitt, William D. Waldbauer, Jacob Venceslau, Sofia S. Sim, Min Sub Zhang, Lichun Boidi, Flavia Jaquelina Plummer, Sydney Díaz, Julia M. Pereira, Inês A. C. Bradley, Alexander S. |
| author_role |
author |
| author2 |
Waldbauer, Jacob Venceslau, Sofia S. Sim, Min Sub Zhang, Lichun Boidi, Flavia Jaquelina Plummer, Sydney Díaz, Julia M. Pereira, Inês A. C. Bradley, Alexander S. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
https://orcid.org/0000-0002-7909-2475 https://orcid.org/0000-0002-0338-6143 https://orcid.org/0000-0001-8774-1351 https://orcid.org/0000-0002-3491-9002 https://orcid.org/0000-0001-7135-8745 |
| dc.subject.none.fl_str_mv |
Chemostat Compound-specific hydrogen isotopes Microbial sulfate reduction Quantitative proteomics Stable sulfur isotopes |
| topic |
Chemostat Compound-specific hydrogen isotopes Microbial sulfate reduction Quantitative proteomics Stable sulfur isotopes |
| dc.description.none.fl_txt_mv |
Fil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America. Fil: Leavitt, William D. Washington University in St. Louis. Department of Earth and Planetary Sciences, Missouri; United States of America. Fil: Waldbauer, Jacob. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America. Fil: Venceslau, Sofia S. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal. Fil: Sim, Min Sub. Seoul National University. School of Earth and Environmental Sciences; South Korea. Fil: Zhang, Lichun. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America. Fil: Flavia Jaquelina Boidi. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America. Fil: Flavia Jaquelina Boidi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina. Fil: Flavia Jaquelina Boidi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Plummer, Sydney. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America. Fil: Díaz, Julia M. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America. Fil: Pereira, Inês A. C. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal. Fil: Bradley, Alexander S. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America. Fil: Bradley, Alexander S. Washington University in St. Louis. Division of Biology and Biomedical Sciences, Missouri; United States of America. Abstract: Microbial sulfate reduction is central to the global carbon cycle and the redox evolu-tion of Earth's surface. Tracking the activity of sulfate reducing microorganisms overspace and time relies on a nuanced understanding of stable sulfur isotope fractiona-tion in the context of the biochemical machinery of the metabolism. Here, we link themagnitude of stable sulfur isotopic fractionation to proteomic and metabolite profilesunder different cellular energetic regimes. When energy availability is limited, cell-specific sulfate respiration rates and net sulfur isotope fractionation inversely covary. Beyond net S isotope fractionation values, we also quantified shifts in protein expres-sion, abundances and isotopic composition of intracellular S metabolites, and lipidstructures and lipid/water H isotope fractionation values. These coupled approachesreveal which protein abundances shift directly as a function of energy flux, those thatvary minimally, and those that may vary independent of energy flux and likely do notcontribute to shifts in S-isotope fractionation. By coupling the bulk S-isotope obser-vations with quantitative proteomics, we provide novel constraints for metabolic iso-tope models. Together, these results lay the foundation for more predictive metabolicfractionation models, alongside interpretations of environmental sulfur and sulfatereducer lipid-H isotope data. info:eu-repo/semantics/publishedVersion Fil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America. Fil: Leavitt, William D. Washington University in St. Louis. Department of Earth and Planetary Sciences, Missouri; United States of America. Fil: Waldbauer, Jacob. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America. Fil: Venceslau, Sofia S. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal. Fil: Sim, Min Sub. Seoul National University. School of Earth and Environmental Sciences; South Korea. Fil: Zhang, Lichun. University of Chicago. Department of the Geophysical Sciences, Illinois; United States of America. Fil: Flavia Jaquelina Boidi. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America. Fil: Flavia Jaquelina Boidi. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; Argentina. Fil: Flavia Jaquelina Boidi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Plummer, Sydney. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America. Fil: Díaz, Julia M. University of California, San Diego, La Jolla. Scripps Institution of Oceanography, California; United States of America. Fil: Pereira, Inês A. C. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier, Oeiras; Portugal. Fil: Bradley, Alexander S. Washington University in St. Louis. Department of Earth and Planetary Sciences; Missouri, United States of America. Fil: Bradley, Alexander S. Washington University in St. Louis. Division of Biology and Biomedical Sciences, Missouri; United States of America. |
| description |
Fil: Leavitt, William D. Dartmouth College. Department of Earth Sciences, Hanover, New Hampshire; United States of America. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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publishedVersion |
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article |
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Leavitt, W. D., Waldbauer, J.,Venceslau, S. S., Sim, M. S., Zhang, L., Boidi, F. J., Plummer, S.,Diaz, J. M., Pereira, I. A. C., & Bradley, A. S. (2024). Energyflux couples sulfur isotope fractionation to proteomic andmetabolite profiles in Desulfovibrio vulgaris. Geobiology, 22,e12600. https://doi.org/10.1111/gbi.12600 1472-4677 http://hdl.handle.net/11086/553191 1472-4669 https://doi.org/10.1111/gbi.12600 |
| identifier_str_mv |
Leavitt, W. D., Waldbauer, J.,Venceslau, S. S., Sim, M. S., Zhang, L., Boidi, F. J., Plummer, S.,Diaz, J. M., Pereira, I. A. C., & Bradley, A. S. (2024). Energyflux couples sulfur isotope fractionation to proteomic andmetabolite profiles in Desulfovibrio vulgaris. Geobiology, 22,e12600. https://doi.org/10.1111/gbi.12600 1472-4677 1472-4669 |
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http://hdl.handle.net/11086/553191 https://doi.org/10.1111/gbi.12600 |
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eng |
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