Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas

Autores
Poca, María; Coomans, Olivia; Urcelay, Roberto Carlos; Zeballos, Sebastián Rodolfo; Bodé, Samuel; Boeckx, Pascal
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: A growing number of studies show a discrepancy between the isotopic composition of xylem water and plant water sources. We tested the effect of arbuscular mycorrhizal fungi (AMF) on the isotopic composition of Acacia caven xylem water. As the most common plant-fungal association, AMF might explain this isotopic mismatch. Methods: Seedlings were grown with and without AMF and irrigated with the same water. After 120 days, stem and soil samples were collected and following cryogenic distillation, H and O isotopic composition of xylem and soil water, as well as irrigation water, was measured. Results: Xylem water of non-mycorrhizal seedlings was significantly depleted in 2H compared to soil water (differences up to −15.6‰). When AMF were present, the depletion was significantly higher and appeared for both H and O (differences up to −24.6‰ for δ2H and − 2.9‰ for δ18O between soil and xylem water). Conclusions: Results suggest that isotopic fractionation occurred during water uptake in this xerophytic species. To explain this, we propose an aquaporin-driven mechanism mediating water transport via transmembrane passage. Furthermore, we show for the first time, that AMF enhance the observed discrimination against heavy isotopes, probably by enforcing water passage through aquaporins. Given their ubiquity, AMF could question the fractionation-free assumption during root water uptake.
Fil: Poca, María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Coomans, Olivia. University College Ghent. Faculty Applied Bioscience Engineering; Bélgica
Fil: Urcelay, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Zeballos, Sebastián Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bodé, Samuel. University of Ghent; Bélgica
Fil: Boeckx, Pascal. University of Ghent; Bélgica
Materia
AQUAPORINS
ECOHYDROLOGY
ISOTOPE FRACTIONATION
MYCORRHIZA
PLANT-SOIL-WATER INTERACTIONS
STABLE ISOTOPES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/135932
Acceder
id CONICETDig_79dcdc69aff05e518eb10e3edbb9f49b
oai_identifier_str oai:ri.conicet.gov.ar:11336/135932
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizasPoca, MaríaCoomans, OliviaUrcelay, Roberto CarlosZeballos, Sebastián RodolfoBodé, SamuelBoeckx, PascalAQUAPORINSECOHYDROLOGYISOTOPE FRACTIONATIONMYCORRHIZAPLANT-SOIL-WATER INTERACTIONSSTABLE ISOTOPEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aim: A growing number of studies show a discrepancy between the isotopic composition of xylem water and plant water sources. We tested the effect of arbuscular mycorrhizal fungi (AMF) on the isotopic composition of Acacia caven xylem water. As the most common plant-fungal association, AMF might explain this isotopic mismatch. Methods: Seedlings were grown with and without AMF and irrigated with the same water. After 120 days, stem and soil samples were collected and following cryogenic distillation, H and O isotopic composition of xylem and soil water, as well as irrigation water, was measured. Results: Xylem water of non-mycorrhizal seedlings was significantly depleted in 2H compared to soil water (differences up to −15.6‰). When AMF were present, the depletion was significantly higher and appeared for both H and O (differences up to −24.6‰ for δ2H and − 2.9‰ for δ18O between soil and xylem water). Conclusions: Results suggest that isotopic fractionation occurred during water uptake in this xerophytic species. To explain this, we propose an aquaporin-driven mechanism mediating water transport via transmembrane passage. Furthermore, we show for the first time, that AMF enhance the observed discrimination against heavy isotopes, probably by enforcing water passage through aquaporins. Given their ubiquity, AMF could question the fractionation-free assumption during root water uptake.Fil: Poca, María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Coomans, Olivia. University College Ghent. Faculty Applied Bioscience Engineering; BélgicaFil: Urcelay, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Zeballos, Sebastián Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Bodé, Samuel. University of Ghent; BélgicaFil: Boeckx, Pascal. University of Ghent; BélgicaSpringer2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/135932Poca, María; Coomans, Olivia; Urcelay, Roberto Carlos; Zeballos, Sebastián Rodolfo; Bodé, Samuel; et al.; Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas; Springer; Plant and Soil; 441; 1-2; 5-2019; 485-4970032-079XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11104-019-04139-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s11104-019-04139-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:31Zoai:ri.conicet.gov.ar:11336/135932instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:31.573CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
title Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
spellingShingle Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
Poca, María
AQUAPORINS
ECOHYDROLOGY
ISOTOPE FRACTIONATION
MYCORRHIZA
PLANT-SOIL-WATER INTERACTIONS
STABLE ISOTOPES
title_short Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
title_full Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
title_fullStr Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
title_full_unstemmed Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
title_sort Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas
dc.creator.none.fl_str_mv Poca, María
Coomans, Olivia
Urcelay, Roberto Carlos
Zeballos, Sebastián Rodolfo
Bodé, Samuel
Boeckx, Pascal
author Poca, María
author_facet Poca, María
Coomans, Olivia
Urcelay, Roberto Carlos
Zeballos, Sebastián Rodolfo
Bodé, Samuel
Boeckx, Pascal
author_role author
author2 Coomans, Olivia
Urcelay, Roberto Carlos
Zeballos, Sebastián Rodolfo
Bodé, Samuel
Boeckx, Pascal
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AQUAPORINS
ECOHYDROLOGY
ISOTOPE FRACTIONATION
MYCORRHIZA
PLANT-SOIL-WATER INTERACTIONS
STABLE ISOTOPES
topic AQUAPORINS
ECOHYDROLOGY
ISOTOPE FRACTIONATION
MYCORRHIZA
PLANT-SOIL-WATER INTERACTIONS
STABLE ISOTOPES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aim: A growing number of studies show a discrepancy between the isotopic composition of xylem water and plant water sources. We tested the effect of arbuscular mycorrhizal fungi (AMF) on the isotopic composition of Acacia caven xylem water. As the most common plant-fungal association, AMF might explain this isotopic mismatch. Methods: Seedlings were grown with and without AMF and irrigated with the same water. After 120 days, stem and soil samples were collected and following cryogenic distillation, H and O isotopic composition of xylem and soil water, as well as irrigation water, was measured. Results: Xylem water of non-mycorrhizal seedlings was significantly depleted in 2H compared to soil water (differences up to −15.6‰). When AMF were present, the depletion was significantly higher and appeared for both H and O (differences up to −24.6‰ for δ2H and − 2.9‰ for δ18O between soil and xylem water). Conclusions: Results suggest that isotopic fractionation occurred during water uptake in this xerophytic species. To explain this, we propose an aquaporin-driven mechanism mediating water transport via transmembrane passage. Furthermore, we show for the first time, that AMF enhance the observed discrimination against heavy isotopes, probably by enforcing water passage through aquaporins. Given their ubiquity, AMF could question the fractionation-free assumption during root water uptake.
Fil: Poca, María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Coomans, Olivia. University College Ghent. Faculty Applied Bioscience Engineering; Bélgica
Fil: Urcelay, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Zeballos, Sebastián Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bodé, Samuel. University of Ghent; Bélgica
Fil: Boeckx, Pascal. University of Ghent; Bélgica
description Aim: A growing number of studies show a discrepancy between the isotopic composition of xylem water and plant water sources. We tested the effect of arbuscular mycorrhizal fungi (AMF) on the isotopic composition of Acacia caven xylem water. As the most common plant-fungal association, AMF might explain this isotopic mismatch. Methods: Seedlings were grown with and without AMF and irrigated with the same water. After 120 days, stem and soil samples were collected and following cryogenic distillation, H and O isotopic composition of xylem and soil water, as well as irrigation water, was measured. Results: Xylem water of non-mycorrhizal seedlings was significantly depleted in 2H compared to soil water (differences up to −15.6‰). When AMF were present, the depletion was significantly higher and appeared for both H and O (differences up to −24.6‰ for δ2H and − 2.9‰ for δ18O between soil and xylem water). Conclusions: Results suggest that isotopic fractionation occurred during water uptake in this xerophytic species. To explain this, we propose an aquaporin-driven mechanism mediating water transport via transmembrane passage. Furthermore, we show for the first time, that AMF enhance the observed discrimination against heavy isotopes, probably by enforcing water passage through aquaporins. Given their ubiquity, AMF could question the fractionation-free assumption during root water uptake.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/135932
Poca, María; Coomans, Olivia; Urcelay, Roberto Carlos; Zeballos, Sebastián Rodolfo; Bodé, Samuel; et al.; Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas; Springer; Plant and Soil; 441; 1-2; 5-2019; 485-497
0032-079X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/135932
identifier_str_mv Poca, María; Coomans, Olivia; Urcelay, Roberto Carlos; Zeballos, Sebastián Rodolfo; Bodé, Samuel; et al.; Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas; Springer; Plant and Soil; 441; 1-2; 5-2019; 485-497
0032-079X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11104-019-04139-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11104-019-04139-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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