Melatonin effect on gingival fibroblast culture from rats : preliminary study
- Autores
- Solá, Verónica; Aguilar, Juan; Grenón, Miriam; Carpentieri, Agata
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.
Fil: Aguilar, Juan. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Introducción a la Física y Química Biológica; Argentina.
Fil: Grenón, Miriam. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.
Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.
Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.
Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina.
Melatonin (MEL), hormone involved in circadian rhythms synchronization, also acts as an antioxidant, immune modulator and promotes bone and fibroblastic formation. Since salivary and serum MEL levels decrease in periodontitis, MEL is proposed as a therapeutic agent for oral cavity. AIM: To investigate the effects of MEL on growing fibroblast (FB) culture from rat gingiva and to compare it with other agents. Methods: A primary culture of maxillary gingiva from Wistar rats in enriched culture medium (CM) was performed. After reaching cell confluence, FB were plated (FB 45000 ml/well) and divided into 3 experimental groups: 1) Control (FB-MC only), 2) FB+collagen (FB-MC + lysate collagen 0.01%) 3) FB+MEL (FBMC + solution MEL 50 nM). To perform the growth curve, cells were suspended with a solution of trypsin (0.05%) and counted with the Neubauer chamber and crystal violet staining for 9 days. ANOVA with Bonferroni comparisons for analysis were used. Results: we found that between 3 and 6 day of culture, FB showed exponential growth. Since seventh day, it was observed a decrease in the number of FB. No significant differences (p > 0.05)among the agents tested were observed. However a trend toward better survival in FB+MEL with respect to the controls and treated with other agents was found. Conclusión: we were able to establish the culture conditions of FB from gum which would be a useful tool for future developments related to oral health. More experiments are needed to verify the role of MEL in the establishment and proliferation of FB.
Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.
Fil: Aguilar, Juan. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Introducción a la Física y Química Biológica; Argentina.
Fil: Grenón, Miriam. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.
Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.
Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.
Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina.
Otras Ciencias Médicas - Materia
-
Melatonin
Celular culture
Periodontal disease - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Universidad Nacional de Córdoba
- OAI Identificador
- oai:rdu.unc.edu.ar:11086/556292
Ver los metadatos del registro completo
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Melatonin effect on gingival fibroblast culture from rats : preliminary studySolá, VerónicaAguilar, JuanGrenón, MiriamCarpentieri, AgataMelatoninCelular culturePeriodontal diseaseFil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.Fil: Aguilar, Juan. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Introducción a la Física y Química Biológica; Argentina.Fil: Grenón, Miriam. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina.Melatonin (MEL), hormone involved in circadian rhythms synchronization, also acts as an antioxidant, immune modulator and promotes bone and fibroblastic formation. Since salivary and serum MEL levels decrease in periodontitis, MEL is proposed as a therapeutic agent for oral cavity. AIM: To investigate the effects of MEL on growing fibroblast (FB) culture from rat gingiva and to compare it with other agents. Methods: A primary culture of maxillary gingiva from Wistar rats in enriched culture medium (CM) was performed. After reaching cell confluence, FB were plated (FB 45000 ml/well) and divided into 3 experimental groups: 1) Control (FB-MC only), 2) FB+collagen (FB-MC + lysate collagen 0.01%) 3) FB+MEL (FBMC + solution MEL 50 nM). To perform the growth curve, cells were suspended with a solution of trypsin (0.05%) and counted with the Neubauer chamber and crystal violet staining for 9 days. ANOVA with Bonferroni comparisons for analysis were used. Results: we found that between 3 and 6 day of culture, FB showed exponential growth. Since seventh day, it was observed a decrease in the number of FB. No significant differences (p > 0.05)among the agents tested were observed. However a trend toward better survival in FB+MEL with respect to the controls and treated with other agents was found. Conclusión: we were able to establish the culture conditions of FB from gum which would be a useful tool for future developments related to oral health. More experiments are needed to verify the role of MEL in the establishment and proliferation of FB.Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.Fil: Aguilar, Juan. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Introducción a la Física y Química Biológica; Argentina.Fil: Grenón, Miriam. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina.Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina.Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina.Otras Ciencias Médicas2015info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/556292info:eu-repo/semantics/openAccessengreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-10-16T09:30:50Zoai:rdu.unc.edu.ar:11086/556292Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-10-16 09:30:51.121Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse |
| dc.title.none.fl_str_mv |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| title |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| spellingShingle |
Melatonin effect on gingival fibroblast culture from rats : preliminary study Solá, Verónica Melatonin Celular culture Periodontal disease |
| title_short |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| title_full |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| title_fullStr |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| title_full_unstemmed |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| title_sort |
Melatonin effect on gingival fibroblast culture from rats : preliminary study |
| dc.creator.none.fl_str_mv |
Solá, Verónica Aguilar, Juan Grenón, Miriam Carpentieri, Agata |
| author |
Solá, Verónica |
| author_facet |
Solá, Verónica Aguilar, Juan Grenón, Miriam Carpentieri, Agata |
| author_role |
author |
| author2 |
Aguilar, Juan Grenón, Miriam Carpentieri, Agata |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Melatonin Celular culture Periodontal disease |
| topic |
Melatonin Celular culture Periodontal disease |
| dc.description.none.fl_txt_mv |
Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina. Fil: Aguilar, Juan. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Introducción a la Física y Química Biológica; Argentina. Fil: Grenón, Miriam. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina. Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina. Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina. Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Melatonin (MEL), hormone involved in circadian rhythms synchronization, also acts as an antioxidant, immune modulator and promotes bone and fibroblastic formation. Since salivary and serum MEL levels decrease in periodontitis, MEL is proposed as a therapeutic agent for oral cavity. AIM: To investigate the effects of MEL on growing fibroblast (FB) culture from rat gingiva and to compare it with other agents. Methods: A primary culture of maxillary gingiva from Wistar rats in enriched culture medium (CM) was performed. After reaching cell confluence, FB were plated (FB 45000 ml/well) and divided into 3 experimental groups: 1) Control (FB-MC only), 2) FB+collagen (FB-MC + lysate collagen 0.01%) 3) FB+MEL (FBMC + solution MEL 50 nM). To perform the growth curve, cells were suspended with a solution of trypsin (0.05%) and counted with the Neubauer chamber and crystal violet staining for 9 days. ANOVA with Bonferroni comparisons for analysis were used. Results: we found that between 3 and 6 day of culture, FB showed exponential growth. Since seventh day, it was observed a decrease in the number of FB. No significant differences (p > 0.05)among the agents tested were observed. However a trend toward better survival in FB+MEL with respect to the controls and treated with other agents was found. Conclusión: we were able to establish the culture conditions of FB from gum which would be a useful tool for future developments related to oral health. More experiments are needed to verify the role of MEL in the establishment and proliferation of FB. Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina. Fil: Aguilar, Juan. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Introducción a la Física y Química Biológica; Argentina. Fil: Grenón, Miriam. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina. Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina. Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “A” de Periodoncia; Argentina. Fil: Carpentieri, Agata. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Otras Ciencias Médicas |
| description |
Fil: Solá, Verónica. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra “B” de Química Biológica; Argentina. |
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2015 |
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2015 |
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