Description and comparison of methods for the determination of anti-sars-COV-2 antibodies

Autores
Aznar, Patricia Marcela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
tesis doctoral
Estado
versión publicada
Colaborador/a o director/a de tesis
Oldano, Ana Verónica
Alcaraz, G. P.
Descripción
Trabajo Integrador Final (Especialista en Bioquímica Clínica Área Inmunología) - - Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, 2022.
Fil: Aznar, Patricia Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
Fil: Aznar, Patricia Marcela. Tucumán. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Laboratorio de Salud Pública. Sección Inmunología; Argentina.
The SARS-COV-2 pandemic had an impact with more than 570 million cases and more than 6.8 million deaths as recorded by the United Nations by July 2022. Within the diagnostic context, a few immunological techniques for the detection of anti-SARS-CoV-2 IgG antibodies emerged, including Chemiluminescent Microparticle Immunoassay (CMIA) and Enzyme-Linked Immunosorbent Assay (ELISA). This study aimed to compare three detection techniques that have different methodological basis. The study was observational, cross-sectional and retrospective in design. Materials and methods: Serum samples were processed by the following methodologies: CMIA and “in-house” ELISA. Results: The methodologies were quantitatively evaluated by the correlation method, and qualitatively by assessing individual and simultaneous sensitivity and specificity. Conclusions: These methodologies were designed in a health emergency context so they do not have a gold standard; thus, different algorithms are proposed to improve both the sensitivity and specificity of the detection of SARS-CoV-2 antibodies.
2024-10-31
Fil: Aznar, Patricia Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
Fil: Aznar, Patricia Marcela. Tucumán. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Laboratorio de Salud Pública. Sección Inmunología; Argentina.
Materia
Anticuerpos
Pruebas serológicas
Pruebas de laboratorio
Virus
Covid 19
Métodos epidemiológicos
Inmunología
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/30139
Acceder
id RDUUNC_b26ec0d966fe721f669c1f67cf005b27
oai_identifier_str oai:rdu.unc.edu.ar:11086/30139
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Description and comparison of methods for the determination of anti-sars-COV-2 antibodiesAznar, Patricia MarcelaAnticuerposPruebas serológicasPruebas de laboratorioVirusCovid 19Métodos epidemiológicosInmunologíaTrabajo Integrador Final (Especialista en Bioquímica Clínica Área Inmunología) - - Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, 2022.Fil: Aznar, Patricia Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Aznar, Patricia Marcela. Tucumán. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Laboratorio de Salud Pública. Sección Inmunología; Argentina.The SARS-COV-2 pandemic had an impact with more than 570 million cases and more than 6.8 million deaths as recorded by the United Nations by July 2022. Within the diagnostic context, a few immunological techniques for the detection of anti-SARS-CoV-2 IgG antibodies emerged, including Chemiluminescent Microparticle Immunoassay (CMIA) and Enzyme-Linked Immunosorbent Assay (ELISA). This study aimed to compare three detection techniques that have different methodological basis. The study was observational, cross-sectional and retrospective in design. Materials and methods: Serum samples were processed by the following methodologies: CMIA and “in-house” ELISA. Results: The methodologies were quantitatively evaluated by the correlation method, and qualitatively by assessing individual and simultaneous sensitivity and specificity. Conclusions: These methodologies were designed in a health emergency context so they do not have a gold standard; thus, different algorithms are proposed to improve both the sensitivity and specificity of the detection of SARS-CoV-2 antibodies.2024-10-31Fil: Aznar, Patricia Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Aznar, Patricia Marcela. Tucumán. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Laboratorio de Salud Pública. Sección Inmunología; Argentina.Oldano, Ana VerónicaAlcaraz, G. P.2022-11-01info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_db06info:ar-repo/semantics/tesisDoctoralapplication/pdfhttp://hdl.handle.net/11086/30139enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-10-16T09:29:23Zoai:rdu.unc.edu.ar:11086/30139Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-10-16 09:29:23.605Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
title Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
spellingShingle Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
Aznar, Patricia Marcela
Anticuerpos
Pruebas serológicas
Pruebas de laboratorio
Virus
Covid 19
Métodos epidemiológicos
Inmunología
title_short Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
title_full Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
title_fullStr Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
title_full_unstemmed Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
title_sort Description and comparison of methods for the determination of anti-sars-COV-2 antibodies
dc.creator.none.fl_str_mv Aznar, Patricia Marcela
author Aznar, Patricia Marcela
author_facet Aznar, Patricia Marcela
author_role author
dc.contributor.none.fl_str_mv Oldano, Ana Verónica
Alcaraz, G. P.
dc.subject.none.fl_str_mv Anticuerpos
Pruebas serológicas
Pruebas de laboratorio
Virus
Covid 19
Métodos epidemiológicos
Inmunología
topic Anticuerpos
Pruebas serológicas
Pruebas de laboratorio
Virus
Covid 19
Métodos epidemiológicos
Inmunología
dc.description.none.fl_txt_mv Trabajo Integrador Final (Especialista en Bioquímica Clínica Área Inmunología) - - Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, 2022.
Fil: Aznar, Patricia Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
Fil: Aznar, Patricia Marcela. Tucumán. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Laboratorio de Salud Pública. Sección Inmunología; Argentina.
The SARS-COV-2 pandemic had an impact with more than 570 million cases and more than 6.8 million deaths as recorded by the United Nations by July 2022. Within the diagnostic context, a few immunological techniques for the detection of anti-SARS-CoV-2 IgG antibodies emerged, including Chemiluminescent Microparticle Immunoassay (CMIA) and Enzyme-Linked Immunosorbent Assay (ELISA). This study aimed to compare three detection techniques that have different methodological basis. The study was observational, cross-sectional and retrospective in design. Materials and methods: Serum samples were processed by the following methodologies: CMIA and “in-house” ELISA. Results: The methodologies were quantitatively evaluated by the correlation method, and qualitatively by assessing individual and simultaneous sensitivity and specificity. Conclusions: These methodologies were designed in a health emergency context so they do not have a gold standard; thus, different algorithms are proposed to improve both the sensitivity and specificity of the detection of SARS-CoV-2 antibodies.
2024-10-31
Fil: Aznar, Patricia Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
Fil: Aznar, Patricia Marcela. Tucumán. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Laboratorio de Salud Pública. Sección Inmunología; Argentina.
description Trabajo Integrador Final (Especialista en Bioquímica Clínica Área Inmunología) - - Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, 2022.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_db06
info:ar-repo/semantics/tesisDoctoral
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11086/30139
url http://hdl.handle.net/11086/30139
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 12.711113

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