Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study

• Autores: Lorenzo, Carmen; Ferreira, Henrique B.; Monteiro, Karina M.; Rosenzvit, Mara C.; Kamenetzky, Laura; Garcia, Héctor H.; Vasquez, Yessika; Naquira, Cesar; Sanchez, Elizabeth; Lorca, Myriam; Contreras, María; Last, Jerry A.; Gonzalez-Sapienza, Gualberto G.
• Año de publicación: 2005
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Journal of Clinical Microbiology, 2005, 43(6), 2764–2770.
• Materia: Echinococcus granulosus; Pruebas Serológicas; Equinococosis
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

The serodiagnosis of hydatid disease is a valuable instrument for clinical diagnosis and epidemiological surveillance of high-risk populations. In the past decade a wealth of reports on the diagnostic performance of numerous antigens have been produced. However, their diagnostic value has been estimated under different conditions, using different serum collection, therefore precluding their direct comparison. Here we report an unbiased comparison of the same batch of six major E. granulosus antigens, namely, hydatid cyst fluid (HCF), native antigen B (AgB), two recombinant AgB subunits, an AgB-derived synthetic peptide, and recombinant cytosolic malate dehydrogenase from E. granulosus (EgMDH), against the same serum collection. The doubleblind analysis was performed using a standardized protocol and receiver operating characteristic (ROC) data analysis by a network of six South American laboratories. High intercenter reproducibility was attained, and the intralaboratory analysis allowed the comparative ranking of the antigen panel. HCF, AgB, and its AgB8/1 subunit exhibited equivalent diagnostic efficiencies, 81.4% 0.5%, 81.3% 0.6%, and 81.9% 2.0%, respectively; with a more favorable balance toward specificity in the case of the last antigen. The diagnostic efficiencies for the other three antigens were 76.8% 6.8%, 69.1% 2.7%, and 66.8% 2.1%, for the peptide, the AgB8/2 subunit, and the EgMDH, respectively. The study also included an analysis of batch-to-batch variation in the diagnostic performance of different HCF regional preparations. Based on these results, a suggested recommendation on the use of these antigens was drawn.
Fil: Lorenzo, Carmen. Instituto de Higiene. Cátedra de Inmunología; Uruguay.
Fil: Ferreira, Henrique B. Universidade Federal do Rio Grande. Laboratório de Biologia Molecular de Cestódeos; Brazil.
Fil: Monteiro, Karina M. Universidade Federal do Rio Grande. Laboratório de Biologia Molecular de Cestódeos; Brazil.
Fil: Rosenzvit, Mara. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Kamenetzky, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Garcia, Héctor H. Instituto de Ciencias Neurológicas. Departamento de Microbiología; Perú.
Fil: Vasquez, Yessika. Instituto de Ciencias Neurológicas. Departamento de Microbiología; Perú.
Fil: Naquira, Cesar. Instituto Nacional de Salud. Laboratorio de Zoonosis; Perú.
Fil: Sanchez, Elizabeth. Instituto Nacional de Salud. Laboratorio de Zoonosis; Perú.
Fil: Lorca, Myriam. Universidad de Chile. Unidad de Parasitología; Chile.
Fil: Contreras, María. Universidad de Chile. Unidad de Parasitología; Chile.
Fil: Last, Jerry A. University of California. Department of Internal Medicine (Pulmonary); Estados Unidos.
Fil: Gonzalez-Sapienza, Gualberto G. Instituto de Higiene. Cátedra de Inmunología; Uruguay.