Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
- Autores
- Martínez, L.D.; Bezard, M.; Brunotto, M.; Dodelson de Kremer, R.
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Creatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes.
publishedVersion - Materia
-
Creatine deficiency
Biomarkers
Saliva
Brain
Chromatography, Gas - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Universidad Nacional de Córdoba
- OAI Identificador
- oai:rdu.unc.edu.ar:11086/4888
Ver los metadatos del registro completo
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2572 |
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Repositorio Digital Universitario (UNC) |
spelling |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.Martínez, L.D.Bezard, M.Brunotto, M.Dodelson de Kremer, R.Creatine deficiencyBiomarkersSalivaBrainChromatography, GasCreatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes.publishedVersionActa Odontológica Latinoamericana : AOL.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfMartínez, L.D, Bezard, M, Brunotto, M, Dodelson de kremer, R. Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Acta Odontol Latinoam. 2016;29(1): 49-53.0326-4815http://hdl.handle.net/11086/4888enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:43:10Zoai:rdu.unc.edu.ar:11086/4888Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:43:10.779Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse |
dc.title.none.fl_str_mv |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
title |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
spellingShingle |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Martínez, L.D. Creatine deficiency Biomarkers Saliva Brain Chromatography, Gas |
title_short |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
title_full |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
title_fullStr |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
title_full_unstemmed |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
title_sort |
Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. |
dc.creator.none.fl_str_mv |
Martínez, L.D. Bezard, M. Brunotto, M. Dodelson de Kremer, R. |
author |
Martínez, L.D. |
author_facet |
Martínez, L.D. Bezard, M. Brunotto, M. Dodelson de Kremer, R. |
author_role |
author |
author2 |
Bezard, M. Brunotto, M. Dodelson de Kremer, R. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Creatine deficiency Biomarkers Saliva Brain Chromatography, Gas |
topic |
Creatine deficiency Biomarkers Saliva Brain Chromatography, Gas |
dc.description.none.fl_txt_mv |
Creatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes. publishedVersion |
description |
Creatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
Martínez, L.D, Bezard, M, Brunotto, M, Dodelson de kremer, R. Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Acta Odontol Latinoam. 2016;29(1): 49-53. 0326-4815 http://hdl.handle.net/11086/4888 |
identifier_str_mv |
Martínez, L.D, Bezard, M, Brunotto, M, Dodelson de kremer, R. Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Acta Odontol Latinoam. 2016;29(1): 49-53. 0326-4815 |
url |
http://hdl.handle.net/11086/4888 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Acta Odontológica Latinoamericana : AOL. |
publisher.none.fl_str_mv |
Acta Odontológica Latinoamericana : AOL. |
dc.source.none.fl_str_mv |
reponame:Repositorio Digital Universitario (UNC) instname:Universidad Nacional de Córdoba instacron:UNC |
reponame_str |
Repositorio Digital Universitario (UNC) |
collection |
Repositorio Digital Universitario (UNC) |
instname_str |
Universidad Nacional de Córdoba |
instacron_str |
UNC |
institution |
UNC |
repository.name.fl_str_mv |
Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba |
repository.mail.fl_str_mv |
oca.unc@gmail.com |
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1844618947836510208 |
score |
13.070432 |