Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.

Autores
Martínez, L.D.; Bezard, M.; Brunotto, M.; Dodelson de Kremer, R.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Creatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes.
publishedVersion
Materia
Creatine deficiency
Biomarkers
Saliva
Brain
Chromatography, Gas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/4888

id RDUUNC_93944e755bea262a31190f0ad7692382
oai_identifier_str oai:rdu.unc.edu.ar:11086/4888
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.Martínez, L.D.Bezard, M.Brunotto, M.Dodelson de Kremer, R.Creatine deficiencyBiomarkersSalivaBrainChromatography, GasCreatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes.publishedVersionActa Odontológica Latinoamericana : AOL.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfMartínez, L.D, Bezard, M, Brunotto, M, Dodelson de kremer, R. Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Acta Odontol Latinoam. 2016;29(1): 49-53.0326-4815http://hdl.handle.net/11086/4888enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:43:10Zoai:rdu.unc.edu.ar:11086/4888Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:43:10.779Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
title Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
spellingShingle Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
Martínez, L.D.
Creatine deficiency
Biomarkers
Saliva
Brain
Chromatography, Gas
title_short Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
title_full Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
title_fullStr Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
title_full_unstemmed Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
title_sort Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects.
dc.creator.none.fl_str_mv Martínez, L.D.
Bezard, M.
Brunotto, M.
Dodelson de Kremer, R.
author Martínez, L.D.
author_facet Martínez, L.D.
Bezard, M.
Brunotto, M.
Dodelson de Kremer, R.
author_role author
author2 Bezard, M.
Brunotto, M.
Dodelson de Kremer, R.
author2_role author
author
author
dc.subject.none.fl_str_mv Creatine deficiency
Biomarkers
Saliva
Brain
Chromatography, Gas
topic Creatine deficiency
Biomarkers
Saliva
Brain
Chromatography, Gas
dc.description.none.fl_txt_mv Creatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes.
publishedVersion
description Creatine (Cr) plays an important role in storage and transmission of phosphate-bound energy. Cerebral creatine deficiency syndromes comprise three inherited defects in Cr biosynthesis and transport. The aim of this study was to investigate whether Cr and Guanidinoacetate (GAA) can be detected in saliva of healthy subjects and to establish the relationship between salivary and plasma levels of these molecules. An adapted gas chromatography (GC) method is described for the quantification of Cr and GAA biomarkers in saliva. Reference values were established for GAA and Cr in saliva. These values were age dependent (p= 0.001). No difference between genders was observed. We detected a difference between GAA and Cr concentrations in saliva and in plasma. The GC method for simultaneous determination of GAA and Cr in human saliva is fast, reliable, sensitive, non-invasive and precise to use as a biochemical approach in early detection of cerebral creatine deficiency syndromes.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Martínez, L.D, Bezard, M, Brunotto, M, Dodelson de kremer, R. Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Acta Odontol Latinoam. 2016;29(1): 49-53.
0326-4815
http://hdl.handle.net/11086/4888
identifier_str_mv Martínez, L.D, Bezard, M, Brunotto, M, Dodelson de kremer, R. Creatine metabolism: detection of creatine and guanidinoacetate in saliva of healthy subjects. Acta Odontol Latinoam. 2016;29(1): 49-53.
0326-4815
url http://hdl.handle.net/11086/4888
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Acta Odontológica Latinoamericana : AOL.
publisher.none.fl_str_mv Acta Odontológica Latinoamericana : AOL.
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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