Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
- Autores
- Clop, P. D.; Marchesini, G. R.; Colpo, P.; Perillo, M. A.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.
Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.
Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase space
Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.
Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.
Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Biofísica - Materia
-
Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Universidad Nacional de Córdoba
- OAI Identificador
- oai:rdu.unc.edu.ar:11086/24368
Ver los metadatos del registro completo
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Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topographyClop, P. D.Marchesini, G. R.Colpo, P.Perillo, M. A.NanobiosensorBeta-galactosidaseSurface plasmon resonanceProteinFil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase spaceFil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.Biofísica2014info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/24368enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:42:01Zoai:rdu.unc.edu.ar:11086/24368Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:42:02.03Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse |
dc.title.none.fl_str_mv |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
title |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
spellingShingle |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography Clop, P. D. Nanobiosensor Beta-galactosidase Surface plasmon resonance Protein |
title_short |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
title_full |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
title_fullStr |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
title_full_unstemmed |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
title_sort |
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography |
dc.creator.none.fl_str_mv |
Clop, P. D. Marchesini, G. R. Colpo, P. Perillo, M. A. |
author |
Clop, P. D. |
author_facet |
Clop, P. D. Marchesini, G. R. Colpo, P. Perillo, M. A. |
author_role |
author |
author2 |
Marchesini, G. R. Colpo, P. Perillo, M. A. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Nanobiosensor Beta-galactosidase Surface plasmon resonance Protein |
topic |
Nanobiosensor Beta-galactosidase Surface plasmon resonance Protein |
dc.description.none.fl_txt_mv |
Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina. Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia. Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia. Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina. The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase space Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina. Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia. Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia. Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina. Biofísica |
description |
Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11086/24368 |
url |
http://hdl.handle.net/11086/24368 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositorio Digital Universitario (UNC) instname:Universidad Nacional de Córdoba instacron:UNC |
reponame_str |
Repositorio Digital Universitario (UNC) |
collection |
Repositorio Digital Universitario (UNC) |
instname_str |
Universidad Nacional de Córdoba |
instacron_str |
UNC |
institution |
UNC |
repository.name.fl_str_mv |
Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba |
repository.mail.fl_str_mv |
oca.unc@gmail.com |
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1844618916989501440 |
score |
13.070432 |