Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography

Autores
Clop, P. D.; Marchesini, G. R.; Colpo, P.; Perillo, M. A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.
Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.
Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase space
Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.
Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.
Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Biofísica
Materia
Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/24368
Acceder
id RDUUNC_8ff137e0667f6fc1a22bd99c88d1533d
oai_identifier_str oai:rdu.unc.edu.ar:11086/24368
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topographyClop, P. D.Marchesini, G. R.Colpo, P.Perillo, M. A.NanobiosensorBeta-galactosidaseSurface plasmon resonanceProteinFil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase spaceFil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.Biofísica2014info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/24368enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-10-23T11:16:19Zoai:rdu.unc.edu.ar:11086/24368Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-10-23 11:16:20.12Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
spellingShingle Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
Clop, P. D.
Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
title_short Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_full Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_fullStr Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_full_unstemmed Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_sort Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
dc.creator.none.fl_str_mv Clop, P. D.
Marchesini, G. R.
Colpo, P.
Perillo, M. A.
author Clop, P. D.
author_facet Clop, P. D.
Marchesini, G. R.
Colpo, P.
Perillo, M. A.
author_role author
author2 Marchesini, G. R.
Colpo, P.
Perillo, M. A.
author2_role author
author
author
dc.subject.none.fl_str_mv Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
topic Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
dc.description.none.fl_txt_mv Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.
Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.
Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase space
Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Fil: Marchesini, G. R. CEO Plasmore S.R.L, Ranco; Italia.
Fil: Colpo, P. JRC IHCP Biosensing on nanostructures, Ispra (VA); Italia.
Fil: Perillo, M. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
Biofísica
description Fil: Clop, P. D. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas: Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11086/24368
url http://hdl.handle.net/11086/24368
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 12.982451

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