Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET

Autores
Brouard, Danny; Ratelle, Olivier; Bracamonte, Angel Guillermo; St-Louis, Maryse; Boudreau, Denis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A biosensor based on a cationic polymer and metal@silica core–shell nanoparticles is demonstrated for the rapid identification of the SRY gene from unamplified human genomic DNA. By amplifying the fluorescence signal triggered by the capture of target molecules, this biosensor avoids the added complexity of enzyme-based chemical amplification, makes genotyping assays faster and less costly and could have important applications in various areas of health sciences.
Fil: Brouard, Danny. Laval University; Canadá
Fil: Ratelle, Olivier. Laval University; Canadá
Fil: Bracamonte, Angel Guillermo. Laval University; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: St-Louis, Maryse. Héma-Québec; Canadá
Fil: Boudreau, Denis. Laval University; Canadá
Materia
Nanobiosensor
Pcr-Free
Genotyping
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25256
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25256
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRETBrouard, DannyRatelle, OlivierBracamonte, Angel GuillermoSt-Louis, MaryseBoudreau, DenisNanobiosensorPcr-FreeGenotypinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A biosensor based on a cationic polymer and metal@silica core–shell nanoparticles is demonstrated for the rapid identification of the SRY gene from unamplified human genomic DNA. By amplifying the fluorescence signal triggered by the capture of target molecules, this biosensor avoids the added complexity of enzyme-based chemical amplification, makes genotyping assays faster and less costly and could have important applications in various areas of health sciences.Fil: Brouard, Danny. Laval University; CanadáFil: Ratelle, Olivier. Laval University; CanadáFil: Bracamonte, Angel Guillermo. Laval University; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: St-Louis, Maryse. Héma-Québec; CanadáFil: Boudreau, Denis. Laval University; CanadáRoyal Society of Chemistry2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25256Brouard, Danny; Ratelle, Olivier; Bracamonte, Angel Guillermo; St-Louis, Maryse; Boudreau, Denis; Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET; Royal Society of Chemistry; Acs Nano; 5; 24; 1-2013; 6896-68991936-0851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/C3AY41428Kinfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2013/ay/c3ay41428k#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:13Zoai:ri.conicet.gov.ar:11336/25256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:13.342CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
title Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
spellingShingle Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
Brouard, Danny
Nanobiosensor
Pcr-Free
Genotyping
title_short Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
title_full Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
title_fullStr Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
title_full_unstemmed Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
title_sort Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET
dc.creator.none.fl_str_mv Brouard, Danny
Ratelle, Olivier
Bracamonte, Angel Guillermo
St-Louis, Maryse
Boudreau, Denis
author Brouard, Danny
author_facet Brouard, Danny
Ratelle, Olivier
Bracamonte, Angel Guillermo
St-Louis, Maryse
Boudreau, Denis
author_role author
author2 Ratelle, Olivier
Bracamonte, Angel Guillermo
St-Louis, Maryse
Boudreau, Denis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Nanobiosensor
Pcr-Free
Genotyping
topic Nanobiosensor
Pcr-Free
Genotyping
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A biosensor based on a cationic polymer and metal@silica core–shell nanoparticles is demonstrated for the rapid identification of the SRY gene from unamplified human genomic DNA. By amplifying the fluorescence signal triggered by the capture of target molecules, this biosensor avoids the added complexity of enzyme-based chemical amplification, makes genotyping assays faster and less costly and could have important applications in various areas of health sciences.
Fil: Brouard, Danny. Laval University; Canadá
Fil: Ratelle, Olivier. Laval University; Canadá
Fil: Bracamonte, Angel Guillermo. Laval University; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: St-Louis, Maryse. Héma-Québec; Canadá
Fil: Boudreau, Denis. Laval University; Canadá
description A biosensor based on a cationic polymer and metal@silica core–shell nanoparticles is demonstrated for the rapid identification of the SRY gene from unamplified human genomic DNA. By amplifying the fluorescence signal triggered by the capture of target molecules, this biosensor avoids the added complexity of enzyme-based chemical amplification, makes genotyping assays faster and less costly and could have important applications in various areas of health sciences.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25256
Brouard, Danny; Ratelle, Olivier; Bracamonte, Angel Guillermo; St-Louis, Maryse; Boudreau, Denis; Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET; Royal Society of Chemistry; Acs Nano; 5; 24; 1-2013; 6896-6899
1936-0851
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25256
identifier_str_mv Brouard, Danny; Ratelle, Olivier; Bracamonte, Angel Guillermo; St-Louis, Maryse; Boudreau, Denis; Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET; Royal Society of Chemistry; Acs Nano; 5; 24; 1-2013; 6896-6899
1936-0851
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/C3AY41428K
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2013/ay/c3ay41428k#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614503226933248
score 13.070432

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