Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease

Autores
Novak, Ivón; Orquera, Abel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
1 p.
Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
The processing and antigen presentation are involved in human macrophage lymphocyte rosette (MLR) and in this phenomenon of multiple immune synapse (IS) the autologous antigens of senescent neutrophils are presented by endocitic way like phagocytosis of cells undergoing apoptosis naturally in the body. In Chagas disease cardiomyopathy T. cruzi is the etiologic agent but also autoimmune phenomena have been described and observed an increment in occurrence of this phenomenon and CD4 PAS-positive lymphocytes producing IFN gamma are involved. Material and methods: healthy human blood samples, anticoagulated with heparin (n = 10) and chagasic samples (n=6) (Blood Bank, UNC) Autologous cultures in TC199 medium (SIGMA, St. Louis, MO). Samples: 48, 72, 96 and 120 h. MLR technique. PAS technique. Samples of MLRs underwent electron microscopy. Results: MLRs of chagasic patients involved more lymphocytes. Significant differences were observed between PAS positive lymphocytes from MLRs (p <0.01). In Chagas cultures were observed plasma cells and neutrophils up to 48 h. Ultrastructure: redistribution of mitochondria in lymphocytes of MLRs, to the IS area. We observed multivesicular, multilamellar and tubular structures in spatial organization of MIIC along time culture in macrophages. In lymphocytes were observed differences in arrangement of chromatin between healthy individuals and Chagas patients as in previous works. Is noted that in cytoplasm were observed double-membrane vesicles to a greater extent in Chagas patients, we postulate costimulator B7 molecules carriers and we are preparing further studies to immunostaining. In all cases observed exosomes, IS and MLR phenomenon greater degree in chagasic patients cultures.
https://www.frontiersin.org/10.3389/conf.fimmu.2013.02.00875/event_abstract
Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Inmunología
Fuente
Novak IT and Orquera AD (2013). Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00875
Materia
Antigen processing and presentation
Ultrastucture
Inmunological Synapses
Macrophages
Lymphocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/16452
Acceder
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oai_identifier_str oai:rdu.unc.edu.ar:11086/16452
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas diseaseNovak, IvónOrquera, AbelAntigen processing and presentationUltrastuctureInmunological SynapsesMacrophagesLymphocytes1 p.Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.The processing and antigen presentation are involved in human macrophage lymphocyte rosette (MLR) and in this phenomenon of multiple immune synapse (IS) the autologous antigens of senescent neutrophils are presented by endocitic way like phagocytosis of cells undergoing apoptosis naturally in the body. In Chagas disease cardiomyopathy T. cruzi is the etiologic agent but also autoimmune phenomena have been described and observed an increment in occurrence of this phenomenon and CD4 PAS-positive lymphocytes producing IFN gamma are involved. Material and methods: healthy human blood samples, anticoagulated with heparin (n = 10) and chagasic samples (n=6) (Blood Bank, UNC) Autologous cultures in TC199 medium (SIGMA, St. Louis, MO). Samples: 48, 72, 96 and 120 h. MLR technique. PAS technique. Samples of MLRs underwent electron microscopy. Results: MLRs of chagasic patients involved more lymphocytes. Significant differences were observed between PAS positive lymphocytes from MLRs (p <0.01). In Chagas cultures were observed plasma cells and neutrophils up to 48 h. Ultrastructure: redistribution of mitochondria in lymphocytes of MLRs, to the IS area. We observed multivesicular, multilamellar and tubular structures in spatial organization of MIIC along time culture in macrophages. In lymphocytes were observed differences in arrangement of chromatin between healthy individuals and Chagas patients as in previous works. Is noted that in cytoplasm were observed double-membrane vesicles to a greater extent in Chagas patients, we postulate costimulator B7 molecules carriers and we are preparing further studies to immunostaining. In all cases observed exosomes, IS and MLR phenomenon greater degree in chagasic patients cultures.https://www.frontiersin.org/10.3389/conf.fimmu.2013.02.00875/event_abstractFil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.InmunologíaInternational Union of Immunological Societies (IUIS)International Union of Immunological Societies (IUIS)2013info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciatext/htmlapplication/pdf1664-3224doi: 10.3389/conf.fimmu.2013.02.00875http://hdl.handle.net/11086/16452Novak IT and Orquera AD (2013). Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00875reponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNCeng11086/14404info:eu-repo/semantics/openAccess2025-10-16T09:31:13Zoai:rdu.unc.edu.ar:11086/16452Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-10-16 09:31:13.278Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
title Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
spellingShingle Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
Novak, Ivón
Antigen processing and presentation
Ultrastucture
Inmunological Synapses
Macrophages
Lymphocytes
title_short Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
title_full Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
title_fullStr Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
title_full_unstemmed Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
title_sort Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease
dc.creator.none.fl_str_mv Novak, Ivón
Orquera, Abel
author Novak, Ivón
author_facet Novak, Ivón
Orquera, Abel
author_role author
author2 Orquera, Abel
author2_role author
dc.subject.none.fl_str_mv Antigen processing and presentation
Ultrastucture
Inmunological Synapses
Macrophages
Lymphocytes
topic Antigen processing and presentation
Ultrastucture
Inmunological Synapses
Macrophages
Lymphocytes
dc.description.none.fl_txt_mv 1 p.
Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
The processing and antigen presentation are involved in human macrophage lymphocyte rosette (MLR) and in this phenomenon of multiple immune synapse (IS) the autologous antigens of senescent neutrophils are presented by endocitic way like phagocytosis of cells undergoing apoptosis naturally in the body. In Chagas disease cardiomyopathy T. cruzi is the etiologic agent but also autoimmune phenomena have been described and observed an increment in occurrence of this phenomenon and CD4 PAS-positive lymphocytes producing IFN gamma are involved. Material and methods: healthy human blood samples, anticoagulated with heparin (n = 10) and chagasic samples (n=6) (Blood Bank, UNC) Autologous cultures in TC199 medium (SIGMA, St. Louis, MO). Samples: 48, 72, 96 and 120 h. MLR technique. PAS technique. Samples of MLRs underwent electron microscopy. Results: MLRs of chagasic patients involved more lymphocytes. Significant differences were observed between PAS positive lymphocytes from MLRs (p <0.01). In Chagas cultures were observed plasma cells and neutrophils up to 48 h. Ultrastructure: redistribution of mitochondria in lymphocytes of MLRs, to the IS area. We observed multivesicular, multilamellar and tubular structures in spatial organization of MIIC along time culture in macrophages. In lymphocytes were observed differences in arrangement of chromatin between healthy individuals and Chagas patients as in previous works. Is noted that in cytoplasm were observed double-membrane vesicles to a greater extent in Chagas patients, we postulate costimulator B7 molecules carriers and we are preparing further studies to immunostaining. In all cases observed exosomes, IS and MLR phenomenon greater degree in chagasic patients cultures.
https://www.frontiersin.org/10.3389/conf.fimmu.2013.02.00875/event_abstract
Fil: Novak, Ivón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Orquera, Abel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Inmunología
description 1 p.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv 1664-3224
doi: 10.3389/conf.fimmu.2013.02.00875
http://hdl.handle.net/11086/16452
identifier_str_mv 1664-3224
doi: 10.3389/conf.fimmu.2013.02.00875
url http://hdl.handle.net/11086/16452
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 11086/14404
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
application/pdf
dc.publisher.none.fl_str_mv International Union of Immunological Societies (IUIS)
International Union of Immunological Societies (IUIS)
publisher.none.fl_str_mv International Union of Immunological Societies (IUIS)
International Union of Immunological Societies (IUIS)
dc.source.none.fl_str_mv Novak IT and Orquera AD (2013). Ultrastructure of human autologous macrophage lymphocyte rosettes in Chagas disease. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00875
reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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