Effect of drugs on virulence factors of candida albicans isolated from oral lesion

Autores
Scatena, María Gabriela; Castillo, Graciela del Valle; Belardinelli, Paola Alejandra; Lehner Rosales, Elena María Paula; Barembaum, Silvina Ruth; Azcurra, Ana Isabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Castillo, Graciela del Valle. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Belardinelli, Paola Alejandra. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología B; Argentina.
Fil: Lehner Rosales, Elena María Paula. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Introducción a la Física y Química Biológica B; Argentina.
Fil: Barembaum, Silvina Ruth. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Física y Química Biológica B; Argentina.
Fil: Azcurra, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.
Candida albicans is an opportunistic fungus widely found in oral cavity, causing stomatological lesions. Chlorhexidine (CLX) and nystatin (NYS) are frequently employed in clinical dentistry. There are evidences of the inhibitory role of aspirin (AAS) on virulence factors for fungal adhesion and infection as lipases (LIP) and biofilm formation (BF). The objective of this study was to evaluate the effect of AAS, CLX and NYS on BF and LIP activity of C. albicans isolated from oral lesions. The strains were isolated from patients with different lesions (chronic candidiasis CC n=6, lichen planus LP n=5, and oral cancer OC n=5) and were identified in chromogenic medium and biochemical tests. A reference strain was employed. BF by XTT method, LIP activity, by rhodamine assay, and inhibition of LIP activity with AAS were assessed. Sub-inhibitory concentrations of CLX, NYS and AAS were used. Data were analyzed by Wilcoxon test (p≤0.05). All isolates showed LIP activity and BF (LIP=1.160.07; BP=305.4 188.7). LIP did not showed significant differences between oral lesions. BF values were higher in OC and CC (p=0.05 y p=0.04, respectively). LIP activity showed lower values with AAS and CLX treatments (p < 0.0001). In OC isolates, the highest values of LIP inhibition were observed (1.88 mM, p<0.0001). BF diminished significantly with CLX (p=0.03). When the oral lesion was considered, AAS treatment showed a diminution of BF in CC (p=0.004) and CLX, in OC (p=0.015). The most important inhibition of virulence factors studied was observed with AAS and CLX. Although that CC and OC strains showed the highest values of LIP and BF, these were the most sensible to the treatment with these drugs. Considering the role of virulence factors in fungal pathogenicity, the present study may mean a contribution in the search of a better therapeutic option.
http://www.ricifa.com.ar
Fil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Castillo, Graciela del Valle. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Belardinelli, Paola Alejandra. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología B; Argentina.
Fil: Lehner Rosales, Elena María Paula. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Introducción a la Física y Química Biológica B; Argentina.
Fil: Barembaum, Silvina Ruth. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Física y Química Biológica B; Argentina.
Fil: Azcurra, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.
Otras Ciencias de la Salud
Materia
Candida
Candida albicans
Aspirin
Nystatin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/23085

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oai_identifier_str oai:rdu.unc.edu.ar:11086/23085
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Effect of drugs on virulence factors of candida albicans isolated from oral lesionScatena, María GabrielaCastillo, Graciela del ValleBelardinelli, Paola AlejandraLehner Rosales, Elena María PaulaBarembaum, Silvina RuthAzcurra, Ana IsabelCandidaCandida albicansAspirinNystatinFil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.Fil: Castillo, Graciela del Valle. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.Fil: Belardinelli, Paola Alejandra. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología B; Argentina.Fil: Lehner Rosales, Elena María Paula. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Introducción a la Física y Química Biológica B; Argentina.Fil: Barembaum, Silvina Ruth. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Física y Química Biológica B; Argentina.Fil: Azcurra, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.Candida albicans is an opportunistic fungus widely found in oral cavity, causing stomatological lesions. Chlorhexidine (CLX) and nystatin (NYS) are frequently employed in clinical dentistry. There are evidences of the inhibitory role of aspirin (AAS) on virulence factors for fungal adhesion and infection as lipases (LIP) and biofilm formation (BF). The objective of this study was to evaluate the effect of AAS, CLX and NYS on BF and LIP activity of C. albicans isolated from oral lesions. The strains were isolated from patients with different lesions (chronic candidiasis CC n=6, lichen planus LP n=5, and oral cancer OC n=5) and were identified in chromogenic medium and biochemical tests. A reference strain was employed. BF by XTT method, LIP activity, by rhodamine assay, and inhibition of LIP activity with AAS were assessed. Sub-inhibitory concentrations of CLX, NYS and AAS were used. Data were analyzed by Wilcoxon test (p&#8804;0.05). All isolates showed LIP activity and BF (LIP=1.16&#61617;0.07; BP=305.4&#61617; 188.7). LIP did not showed significant differences between oral lesions. BF values were higher in OC and CC (p=0.05 y p=0.04, respectively). LIP activity showed lower values with AAS and CLX treatments (p < 0.0001). In OC isolates, the highest values of LIP inhibition were observed (1.88 mM, p<0.0001). BF diminished significantly with CLX (p=0.03). When the oral lesion was considered, AAS treatment showed a diminution of BF in CC (p=0.004) and CLX, in OC (p=0.015). The most important inhibition of virulence factors studied was observed with AAS and CLX. Although that CC and OC strains showed the highest values of LIP and BF, these were the most sensible to the treatment with these drugs. Considering the role of virulence factors in fungal pathogenicity, the present study may mean a contribution in the search of a better therapeutic option.http://www.ricifa.com.arFil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.Fil: Castillo, Graciela del Valle. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.Fil: Belardinelli, Paola Alejandra. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología B; Argentina.Fil: Lehner Rosales, Elena María Paula. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Introducción a la Física y Química Biológica B; Argentina.Fil: Barembaum, Silvina Ruth. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Física y Química Biológica B; Argentina.Fil: Azcurra, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.Otras Ciencias de la Salud2014info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/23085enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-04T12:32:00Zoai:rdu.unc.edu.ar:11086/23085Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-04 12:32:01.145Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Effect of drugs on virulence factors of candida albicans isolated from oral lesion
title Effect of drugs on virulence factors of candida albicans isolated from oral lesion
spellingShingle Effect of drugs on virulence factors of candida albicans isolated from oral lesion
Scatena, María Gabriela
Candida
Candida albicans
Aspirin
Nystatin
title_short Effect of drugs on virulence factors of candida albicans isolated from oral lesion
title_full Effect of drugs on virulence factors of candida albicans isolated from oral lesion
title_fullStr Effect of drugs on virulence factors of candida albicans isolated from oral lesion
title_full_unstemmed Effect of drugs on virulence factors of candida albicans isolated from oral lesion
title_sort Effect of drugs on virulence factors of candida albicans isolated from oral lesion
dc.creator.none.fl_str_mv Scatena, María Gabriela
Castillo, Graciela del Valle
Belardinelli, Paola Alejandra
Lehner Rosales, Elena María Paula
Barembaum, Silvina Ruth
Azcurra, Ana Isabel
author Scatena, María Gabriela
author_facet Scatena, María Gabriela
Castillo, Graciela del Valle
Belardinelli, Paola Alejandra
Lehner Rosales, Elena María Paula
Barembaum, Silvina Ruth
Azcurra, Ana Isabel
author_role author
author2 Castillo, Graciela del Valle
Belardinelli, Paola Alejandra
Lehner Rosales, Elena María Paula
Barembaum, Silvina Ruth
Azcurra, Ana Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Candida
Candida albicans
Aspirin
Nystatin
topic Candida
Candida albicans
Aspirin
Nystatin
dc.description.none.fl_txt_mv Fil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Castillo, Graciela del Valle. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Belardinelli, Paola Alejandra. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología B; Argentina.
Fil: Lehner Rosales, Elena María Paula. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Introducción a la Física y Química Biológica B; Argentina.
Fil: Barembaum, Silvina Ruth. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Física y Química Biológica B; Argentina.
Fil: Azcurra, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.
Candida albicans is an opportunistic fungus widely found in oral cavity, causing stomatological lesions. Chlorhexidine (CLX) and nystatin (NYS) are frequently employed in clinical dentistry. There are evidences of the inhibitory role of aspirin (AAS) on virulence factors for fungal adhesion and infection as lipases (LIP) and biofilm formation (BF). The objective of this study was to evaluate the effect of AAS, CLX and NYS on BF and LIP activity of C. albicans isolated from oral lesions. The strains were isolated from patients with different lesions (chronic candidiasis CC n=6, lichen planus LP n=5, and oral cancer OC n=5) and were identified in chromogenic medium and biochemical tests. A reference strain was employed. BF by XTT method, LIP activity, by rhodamine assay, and inhibition of LIP activity with AAS were assessed. Sub-inhibitory concentrations of CLX, NYS and AAS were used. Data were analyzed by Wilcoxon test (p&#8804;0.05). All isolates showed LIP activity and BF (LIP=1.16&#61617;0.07; BP=305.4&#61617; 188.7). LIP did not showed significant differences between oral lesions. BF values were higher in OC and CC (p=0.05 y p=0.04, respectively). LIP activity showed lower values with AAS and CLX treatments (p < 0.0001). In OC isolates, the highest values of LIP inhibition were observed (1.88 mM, p<0.0001). BF diminished significantly with CLX (p=0.03). When the oral lesion was considered, AAS treatment showed a diminution of BF in CC (p=0.004) and CLX, in OC (p=0.015). The most important inhibition of virulence factors studied was observed with AAS and CLX. Although that CC and OC strains showed the highest values of LIP and BF, these were the most sensible to the treatment with these drugs. Considering the role of virulence factors in fungal pathogenicity, the present study may mean a contribution in the search of a better therapeutic option.
http://www.ricifa.com.ar
Fil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Castillo, Graciela del Valle. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
Fil: Belardinelli, Paola Alejandra. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología B; Argentina.
Fil: Lehner Rosales, Elena María Paula. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Introducción a la Física y Química Biológica B; Argentina.
Fil: Barembaum, Silvina Ruth. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Física y Química Biológica B; Argentina.
Fil: Azcurra, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.
Otras Ciencias de la Salud
description Fil: Scatena, María Gabriela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica B; Argentina.
publishDate 2014
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