Salinity response and genetic variability in clones of panicum coloratum to breeding purposes

• Autores: Ribotta, Andrea Noemí; López Colomba, Eliana; Tommasino, Exequiel; Fiol de Cúneo, Marta; Griffa, Sabrina; Quiroga, María Angélica; Carloni, Edgardo José; Grunberg, Karina
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: documento de conferencia
• Materia: Salinity; Genetic variability; Panicum coloratum; ISSR
• Institución: Universidad Nacional de Córdoba

Fil: Ribotta, Andrea Noemí. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: López Colomba, Eliana. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Tommasino, Exequiel. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Fiol de Cúneo, Marta. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Griffa, Sabrina. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Quiroga, María Angélica. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Carloni, Edgardo José. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Grunberg, Karina. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Panicum coloratum is a cross-pollinated perennial grass native of South Africa that has been introduced to different parts of the world including Argentina.In this country, this species has been shown good forage quality and production. The continuous increase in the area dedicated to agriculture has displaced livestock production to areas with adverse environmental conditions. Therefore, a breeding program to increase environmental stress tolerance in P. coloratum is actually been developed for INTA, with emphasis in enhancing salinity tolerance. The aim of this work was to evaluate salt response of clones through morpho-physiological traits and to determinate genetic variability by ISSR markers. The plant material consisted of 18 clones derived from two accessions of different source. These clones were previously selected for Good performance of agro-morphological traits. The salt assay was conducted in a greenhouse under hydroponic conditions with two treatments (0 and 300 mMNaCl). Different traits contributing to biomass productionand traits related to physiological state as malondialdehyde content, the ability to reduce iron, chlorophyll content, total membrane integrity (MI),total soluble sugars, total protein, relative content of water (RCW) and ions Na+, K+ and Mg2+were evaluated. An index that measure the effect generated by salt stress respect to control for each variable was estimated anda multivariate Principal Components Analysis was performed.. Groups of differential behavior in salt stress conditions were obtained by this analysis. Groups with more biomass production were the least affected to salt stress, and this was related to lower damage in the traits MI, CRA and Na+/K+. To performed DNA extraction, 100 mg of young leaves of each clone were used. A total of 28 ISSR primers were screened initially and 23 ISSR primers that produced clear and reproducible fragments were selected. Of the total bands obtained, 443 (95.67%) were polymorphic.The results obtained in this work showed differential response in salt stress and genetic diversity among clones of Panicum coloratum.This is important in a breeding program for their future use as parents in the generation of new germplasm.
Fil: Ribotta, Andrea Noemí. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: López Colomba, Eliana. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Tommasino, Exequiel. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Fiol de Cúneo, Marta. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Griffa, Sabrina. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Quiroga, María Angélica. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Carloni, Edgardo José. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Grunberg, Karina. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Otras Ciencias Agrícolas