Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides

Autores
Pires, Natalia S.; Lascano, Cecilia; Ousset, Julia; Ceschin, Danilo G.; Venturino, Andrés
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae.
Fil: Pires, Natalia S. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Pires, Natalia S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Lascano, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Lascano, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Ousset, Julia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Ousset, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Ceschin, Danilo G. Centro de Investigación en Medicina Traslacional “Severo R. Amuchástegui; Argentina.
Fil: Ceschin, Danilo G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra ; Argentina.
Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fuente
Scientific Reports 12:17712
Materia
Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Repositorio Digital Institucional (UNCo)
Institución
Universidad Nacional del Comahue
OAI Identificador
oai:rdi.uncoma.edu.ar:uncomaid/17808
Acceder
id RDIUNCO_04b47367be44e38b60ae587548b50049
oai_identifier_str oai:rdi.uncoma.edu.ar:uncomaid/17808
network_acronym_str RDIUNCO
repository_id_str 7108
network_name_str Repositorio Digital Institucional (UNCo)
spelling Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticidesPires, Natalia S.Lascano, CeciliaOusset, JuliaCeschin, Danilo G.Venturino, AndrésToxicogenomicRhinella arenarumBiología molecularCiencias de la Tierra y Medio AmbienteCiencias Agrarias y ForestalesTranscriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae.Fil: Pires, Natalia S. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Pires, Natalia S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Fil: Lascano, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Lascano, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Fil: Ousset, Julia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Ousset, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Fil: Ceschin, Danilo G. Centro de Investigación en Medicina Traslacional “Severo R. Amuchástegui; Argentina.Fil: Ceschin, Danilo G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra ; Argentina.Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; ArgentinaFil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Nature Publishing Group2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf2045-2322http://rdi.uncoma.edu.ar/handle/uncomaid/17808Scientific Reports 12:17712reponame:Repositorio Digital Institucional (UNCo)instname:Universidad Nacional del Comahueenghttps://doi.org/10.1038/s41598-022-21748-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/2025-09-04T11:12:48Zoai:rdi.uncoma.edu.ar:uncomaid/17808instacron:UNCoInstitucionalhttp://rdi.uncoma.edu.ar/Universidad públicaNo correspondehttp://rdi.uncoma.edu.ar/oaimirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:71082025-09-04 11:12:48.989Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahuefalse
dc.title.none.fl_str_mv Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
spellingShingle Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
Pires, Natalia S.
Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
title_short Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_full Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_fullStr Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_full_unstemmed Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
title_sort Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides
dc.creator.none.fl_str_mv Pires, Natalia S.
Lascano, Cecilia
Ousset, Julia
Ceschin, Danilo G.
Venturino, Andrés
author Pires, Natalia S.
author_facet Pires, Natalia S.
Lascano, Cecilia
Ousset, Julia
Ceschin, Danilo G.
Venturino, Andrés
author_role author
author2 Lascano, Cecilia
Ousset, Julia
Ceschin, Danilo G.
Venturino, Andrés
author2_role author
author
author
author
dc.subject.none.fl_str_mv Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
topic Toxicogenomic
Rhinella arenarum
Biología molecular
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
dc.description.none.fl_txt_mv Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae.
Fil: Pires, Natalia S. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Pires, Natalia S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Lascano, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Lascano, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Ousset, Julia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Ousset, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Ceschin, Danilo G. Centro de Investigación en Medicina Traslacional “Severo R. Amuchástegui; Argentina.
Fil: Ceschin, Danilo G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra ; Argentina.
Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
description Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv 2045-2322
http://rdi.uncoma.edu.ar/handle/uncomaid/17808
identifier_str_mv 2045-2322
url http://rdi.uncoma.edu.ar/handle/uncomaid/17808
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1038/s41598-022-21748-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific Reports 12:17712
reponame:Repositorio Digital Institucional (UNCo)
instname:Universidad Nacional del Comahue
reponame_str Repositorio Digital Institucional (UNCo)
collection Repositorio Digital Institucional (UNCo)
instname_str Universidad Nacional del Comahue
repository.name.fl_str_mv Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahue
repository.mail.fl_str_mv mirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.ar
_version_ 1842344057046564864
score 12.623145

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