Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads

Autores
Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; Iruzubieta Villagra, Ana Lucrecia; Fernandez, Silvia Nelida
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg.
Fil: Medina, Marcela Fatima. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cosci, Aldo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cisint, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Crespo, Claudia Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ramos, Inés del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Iruzubieta Villagra, Ana Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fernandez, Silvia Nelida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Cadmium
Gonads
Reproduction
Rhinella Arenarum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61457
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61457
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonadsMedina, Marcela FatimaCosci, AldoCisint, Susana BeatrizCrespo, Claudia AlejandraRamos, Inés del CarmenIruzubieta Villagra, Ana LucreciaFernandez, Silvia NelidaCadmiumGonadsReproductionRhinella Arenarumhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg.Fil: Medina, Marcela Fatima. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cosci, Aldo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cisint, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Crespo, Claudia Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Ramos, Inés del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Iruzubieta Villagra, Ana Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fernandez, Silvia Nelida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61457Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; et al.; Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads; Elsevier; Tissue & Cell; 44; 6; 9-2012; 418-4260040-8166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0040816612000791info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tice.2012.08.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:32Zoai:ri.conicet.gov.ar:11336/61457instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:33.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
title Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
spellingShingle Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
Medina, Marcela Fatima
Cadmium
Gonads
Reproduction
Rhinella Arenarum
title_short Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
title_full Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
title_fullStr Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
title_full_unstemmed Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
title_sort Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
dc.creator.none.fl_str_mv Medina, Marcela Fatima
Cosci, Aldo
Cisint, Susana Beatriz
Crespo, Claudia Alejandra
Ramos, Inés del Carmen
Iruzubieta Villagra, Ana Lucrecia
Fernandez, Silvia Nelida
author Medina, Marcela Fatima
author_facet Medina, Marcela Fatima
Cosci, Aldo
Cisint, Susana Beatriz
Crespo, Claudia Alejandra
Ramos, Inés del Carmen
Iruzubieta Villagra, Ana Lucrecia
Fernandez, Silvia Nelida
author_role author
author2 Cosci, Aldo
Cisint, Susana Beatriz
Crespo, Claudia Alejandra
Ramos, Inés del Carmen
Iruzubieta Villagra, Ana Lucrecia
Fernandez, Silvia Nelida
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cadmium
Gonads
Reproduction
Rhinella Arenarum
topic Cadmium
Gonads
Reproduction
Rhinella Arenarum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg.
Fil: Medina, Marcela Fatima. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cosci, Aldo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cisint, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Crespo, Claudia Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ramos, Inés del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Iruzubieta Villagra, Ana Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fernandez, Silvia Nelida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61457
Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; et al.; Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads; Elsevier; Tissue & Cell; 44; 6; 9-2012; 418-426
0040-8166
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61457
identifier_str_mv Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; et al.; Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads; Elsevier; Tissue & Cell; 44; 6; 9-2012; 418-426
0040-8166
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0040816612000791
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tice.2012.08.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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