Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads
- Autores
- Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; Iruzubieta Villagra, Ana Lucrecia; Fernandez, Silvia Nelida
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg.
Fil: Medina, Marcela Fatima. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cosci, Aldo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cisint, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Crespo, Claudia Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ramos, Inés del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Iruzubieta Villagra, Ana Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fernandez, Silvia Nelida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina - Materia
-
Cadmium
Gonads
Reproduction
Rhinella Arenarum - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/61457
Ver los metadatos del registro completo
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Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonadsMedina, Marcela FatimaCosci, AldoCisint, Susana BeatrizCrespo, Claudia AlejandraRamos, Inés del CarmenIruzubieta Villagra, Ana LucreciaFernandez, Silvia NelidaCadmiumGonadsReproductionRhinella Arenarumhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg.Fil: Medina, Marcela Fatima. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cosci, Aldo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cisint, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Crespo, Claudia Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Ramos, Inés del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Iruzubieta Villagra, Ana Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fernandez, Silvia Nelida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61457Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; et al.; Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads; Elsevier; Tissue & Cell; 44; 6; 9-2012; 418-4260040-8166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0040816612000791info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tice.2012.08.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:23Zoai:ri.conicet.gov.ar:11336/61457instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:23.772CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
title |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
spellingShingle |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads Medina, Marcela Fatima Cadmium Gonads Reproduction Rhinella Arenarum |
title_short |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
title_full |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
title_fullStr |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
title_full_unstemmed |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
title_sort |
Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads |
dc.creator.none.fl_str_mv |
Medina, Marcela Fatima Cosci, Aldo Cisint, Susana Beatriz Crespo, Claudia Alejandra Ramos, Inés del Carmen Iruzubieta Villagra, Ana Lucrecia Fernandez, Silvia Nelida |
author |
Medina, Marcela Fatima |
author_facet |
Medina, Marcela Fatima Cosci, Aldo Cisint, Susana Beatriz Crespo, Claudia Alejandra Ramos, Inés del Carmen Iruzubieta Villagra, Ana Lucrecia Fernandez, Silvia Nelida |
author_role |
author |
author2 |
Cosci, Aldo Cisint, Susana Beatriz Crespo, Claudia Alejandra Ramos, Inés del Carmen Iruzubieta Villagra, Ana Lucrecia Fernandez, Silvia Nelida |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Cadmium Gonads Reproduction Rhinella Arenarum |
topic |
Cadmium Gonads Reproduction Rhinella Arenarum |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg. Fil: Medina, Marcela Fatima. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Cosci, Aldo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Cisint, Susana Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Crespo, Claudia Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Ramos, Inés del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Iruzubieta Villagra, Ana Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Fernandez, Silvia Nelida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina |
description |
This study was to determine the lethal dose 50 (LD50) of CdCl2 in adult Rhinella arenarum and analyzed the effect of two sublethal doses (0.5 and 5mg/kg) of the xenobiotic in gonads. The 48h LD50 were 50.0 and 49.8mg/kg for males and females respectively. Alterations in the ovary were evidenced by nuclear pleomorphism and cytoplasmic vacuolization of the oocytes at the early stages of development with the highest dose and an increase in the population of atretic oocytes. In the interstitial tissue we noticed congestion, edema and fibroblast proliferation. The nuclear maturation of the oocytes was affected by the xenobiotic in a dose- and time-dependent manner. In males, treatment with 5mg/kg of cadmium (Cd) caused a decrease in the concentration, viability and straight progressive motility of sperm while there was an increase in immotile sperm. Testis histopathology revealed dilated seminiferous tubules, disappearance of cysts, tissue disorganization and leukocyte infiltration. Numerous germ cells showed hydropic tumefaction or signs of focal necrosis. The Cd content in animals intoxicated gonads with the highest sublethal dose was significantly higher than in the control. Results indicate that R. arenarum gonads are target for the xenobiotic, compromising the formation of gametes competent for fertilization, the effective CdCl2 dose being 5mg/kg. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/61457 Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; et al.; Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads; Elsevier; Tissue & Cell; 44; 6; 9-2012; 418-426 0040-8166 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/61457 |
identifier_str_mv |
Medina, Marcela Fatima; Cosci, Aldo; Cisint, Susana Beatriz; Crespo, Claudia Alejandra; Ramos, Inés del Carmen; et al.; Histopathological and biological studies of the effect of cadmium on Rhinella arenarum gonads; Elsevier; Tissue & Cell; 44; 6; 9-2012; 418-426 0040-8166 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0040816612000791 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tice.2012.08.005 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268921120423936 |
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13.13397 |