Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds

Autores
De Rossi, Roberto Luis; Reis, Erlei Melo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Northern corn leaf blight, caused by Exserohilum turcicum (Et), is a disease of widespread occurrence in regions where corn, sweetcorn and popcorn are grown. This disease has great potential to cause damage and has been studied for years, but the association of its causal agent with seeds remains unconfirmed. Thus, the availability of a sensitive method to detect and quantify the inoculum in seeds, even at low incidence, is essential. The aim of this study was to develop a method to detect and quantify the presence of the fungus infecting and infesting corn and popcorn seeds. Artificially and naturally infected seeds were employed to develop the medium. The semi-selective medium was composed of carbendazim (active ingredient) (60 mg/L), captan (30 mg/L), streptomycin sulfate (500 mg/L) and neomycin sulfate (600 mg/L) aggregated to the medium lactose casein hydrolysate agar medium. By using this, Et was detected in naturally infected corn seeds, showing 0.124% incidence, in four out of ten analyzed samples. In addition, 1.04 conidia were detected per infested seed. By means of isolation, pathogenicity test, morphological characterization and comparison with descriptions of the species in the literature, the fungus isolated from the seeds was confirmed to be Et. Both infection and infestation were considered low; thus, for studies of Et detection in corn seeds, the use of semi-selective medium and more than 1,200 seeds/sample is suggested.
Fil: De Rossi, Roberto Luis. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fuente
De Rossi, Roberto Luis ORCID: https://orcid.org/0000-0003-0130-5415 and Reis, Erlei Melo (2014) Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds. Summa Phytopathologica, 40 (2). pp. 163-167. ISSN 0100-5405
Materia
SB Cultura de la planta
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Repositorio
Producción Académica (UCC)
Institución
Universidad Católica de Córdoba
OAI Identificador
oai:pa.bibdigital.uccor.edu.ar:1407

id PAUCC_771b0c122b4707d93631d796e69ed933
oai_identifier_str oai:pa.bibdigital.uccor.edu.ar:1407
network_acronym_str PAUCC
repository_id_str 2718
network_name_str Producción Académica (UCC)
spelling Semi-selective culture medium for Exserohilum turcicum isolation from corn seedsDe Rossi, Roberto LuisReis, Erlei MeloSB Cultura de la plantaNorthern corn leaf blight, caused by Exserohilum turcicum (Et), is a disease of widespread occurrence in regions where corn, sweetcorn and popcorn are grown. This disease has great potential to cause damage and has been studied for years, but the association of its causal agent with seeds remains unconfirmed. Thus, the availability of a sensitive method to detect and quantify the inoculum in seeds, even at low incidence, is essential. The aim of this study was to develop a method to detect and quantify the presence of the fungus infecting and infesting corn and popcorn seeds. Artificially and naturally infected seeds were employed to develop the medium. The semi-selective medium was composed of carbendazim (active ingredient) (60 mg/L), captan (30 mg/L), streptomycin sulfate (500 mg/L) and neomycin sulfate (600 mg/L) aggregated to the medium lactose casein hydrolysate agar medium. By using this, Et was detected in naturally infected corn seeds, showing 0.124% incidence, in four out of ten analyzed samples. In addition, 1.04 conidia were detected per infested seed. By means of isolation, pathogenicity test, morphological characterization and comparison with descriptions of the species in the literature, the fungus isolated from the seeds was confirmed to be Et. Both infection and infestation were considered low; thus, for studies of Et detection in corn seeds, the use of semi-selective medium and more than 1,200 seeds/sample is suggested.Fil: De Rossi, Roberto Luis. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaAsociación de Fitopatología del Estado de São Paulo (APF)2014-06-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://pa.bibdigital.ucc.edu.ar/1407/1/A_DeRossi.pdf De Rossi, Roberto Luis ORCID: https://orcid.org/0000-0003-0130-5415 <https://orcid.org/0000-0003-0130-5415> and Reis, Erlei Melo (2014) Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds. Summa Phytopathologica, 40 (2). pp. 163-167. ISSN 0100-5405 reponame:Producción Académica (UCC)instname:Universidad Católica de Córdobaenghttp://pa.bibdigital.ucc.edu.ar/1407/http://www.summanet.com.br/summanet-site/site/revista.phpinfo:eu-repo/semantics/altIdentifier/doi/10.1590/0100-5405/1925info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es2025-09-29T14:29:03Zoai:pa.bibdigital.uccor.edu.ar:1407instacron:UCCInstitucionalhttp://pa.bibdigital.uccor.edu.ar/Universidad privadaNo correspondehttp://pa.bibdigital.uccor.edu.ar/cgi/oai2bibdir@uccor.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:27182025-09-29 14:29:04.023Producción Académica (UCC) - Universidad Católica de Córdobafalse
dc.title.none.fl_str_mv Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
title Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
spellingShingle Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
De Rossi, Roberto Luis
SB Cultura de la planta
title_short Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
title_full Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
title_fullStr Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
title_full_unstemmed Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
title_sort Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds
dc.creator.none.fl_str_mv De Rossi, Roberto Luis
Reis, Erlei Melo
author De Rossi, Roberto Luis
author_facet De Rossi, Roberto Luis
Reis, Erlei Melo
author_role author
author2 Reis, Erlei Melo
author2_role author
dc.subject.none.fl_str_mv SB Cultura de la planta
topic SB Cultura de la planta
dc.description.none.fl_txt_mv Northern corn leaf blight, caused by Exserohilum turcicum (Et), is a disease of widespread occurrence in regions where corn, sweetcorn and popcorn are grown. This disease has great potential to cause damage and has been studied for years, but the association of its causal agent with seeds remains unconfirmed. Thus, the availability of a sensitive method to detect and quantify the inoculum in seeds, even at low incidence, is essential. The aim of this study was to develop a method to detect and quantify the presence of the fungus infecting and infesting corn and popcorn seeds. Artificially and naturally infected seeds were employed to develop the medium. The semi-selective medium was composed of carbendazim (active ingredient) (60 mg/L), captan (30 mg/L), streptomycin sulfate (500 mg/L) and neomycin sulfate (600 mg/L) aggregated to the medium lactose casein hydrolysate agar medium. By using this, Et was detected in naturally infected corn seeds, showing 0.124% incidence, in four out of ten analyzed samples. In addition, 1.04 conidia were detected per infested seed. By means of isolation, pathogenicity test, morphological characterization and comparison with descriptions of the species in the literature, the fungus isolated from the seeds was confirmed to be Et. Both infection and infestation were considered low; thus, for studies of Et detection in corn seeds, the use of semi-selective medium and more than 1,200 seeds/sample is suggested.
Fil: De Rossi, Roberto Luis. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
description Northern corn leaf blight, caused by Exserohilum turcicum (Et), is a disease of widespread occurrence in regions where corn, sweetcorn and popcorn are grown. This disease has great potential to cause damage and has been studied for years, but the association of its causal agent with seeds remains unconfirmed. Thus, the availability of a sensitive method to detect and quantify the inoculum in seeds, even at low incidence, is essential. The aim of this study was to develop a method to detect and quantify the presence of the fungus infecting and infesting corn and popcorn seeds. Artificially and naturally infected seeds were employed to develop the medium. The semi-selective medium was composed of carbendazim (active ingredient) (60 mg/L), captan (30 mg/L), streptomycin sulfate (500 mg/L) and neomycin sulfate (600 mg/L) aggregated to the medium lactose casein hydrolysate agar medium. By using this, Et was detected in naturally infected corn seeds, showing 0.124% incidence, in four out of ten analyzed samples. In addition, 1.04 conidia were detected per infested seed. By means of isolation, pathogenicity test, morphological characterization and comparison with descriptions of the species in the literature, the fungus isolated from the seeds was confirmed to be Et. Both infection and infestation were considered low; thus, for studies of Et detection in corn seeds, the use of semi-selective medium and more than 1,200 seeds/sample is suggested.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/1407/1/A_DeRossi.pdf
url http://pa.bibdigital.ucc.edu.ar/1407/1/A_DeRossi.pdf
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/1407/
http://www.summanet.com.br/summanet-site/site/revista.php
info:eu-repo/semantics/altIdentifier/doi/10.1590/0100-5405/1925
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación de Fitopatología del Estado de São Paulo (APF)
publisher.none.fl_str_mv Asociación de Fitopatología del Estado de São Paulo (APF)
dc.source.none.fl_str_mv De Rossi, Roberto Luis ORCID: https://orcid.org/0000-0003-0130-5415 <https://orcid.org/0000-0003-0130-5415> and Reis, Erlei Melo (2014) Semi-selective culture medium for Exserohilum turcicum isolation from corn seeds. Summa Phytopathologica, 40 (2). pp. 163-167. ISSN 0100-5405
reponame:Producción Académica (UCC)
instname:Universidad Católica de Córdoba
reponame_str Producción Académica (UCC)
collection Producción Académica (UCC)
instname_str Universidad Católica de Córdoba
repository.name.fl_str_mv Producción Académica (UCC) - Universidad Católica de Córdoba
repository.mail.fl_str_mv bibdir@uccor.edu.ar
_version_ 1844621578536484864
score 12.559606