Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences

Autores
Muntaabski, Irina; Russo, Romina Maria; Liendo, María Clara; Palacio, María Alejandra; Cladera, Jorge Luis; Lanzavecchia, Silvia Beatriz; Scannapieco, Alejandra Carla
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor.
Instituto de Genética
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liendo, Marí­a Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Parasitology Research 119 (2) : 411-421 (Febrero 2020)
Materia
Genetic Variation
Genetic Markers
Variación Genética
Marcadores Genéticos
Apis mellifera
Varroa destructor
Argentina
Haplotypes
Heteroplasmy
Haplotipos
Heteroplasmia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7894
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7894
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spelling Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequencesMuntaabski, IrinaRusso, Romina MariaLiendo, María ClaraPalacio, María AlejandraCladera, Jorge LuisLanzavecchia, Silvia BeatrizScannapieco, Alejandra CarlaGenetic VariationGenetic MarkersVariación GenéticaMarcadores GenéticosApis melliferaVarroa destructorArgentinaHaplotypesHeteroplasmyHaplotiposHeteroplasmiaVarroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor.Instituto de GenéticaFil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liendo, Marí­a Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2020-09-15T11:35:37Z2020-09-15T11:35:37Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7894https://link.springer.com/article/10.1007/s00436-019-06591-51432-1955https://doi.org/10.1007/s00436-019-06591-5Parasitology Research 119 (2) : 411-421 (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena.info:eu-repo/semantics/restrictedAccess2025-10-23T11:17:22Zoai:localhost:20.500.12123/7894instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:22.716INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
title Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
spellingShingle Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
Muntaabski, Irina
Genetic Variation
Genetic Markers
Variación Genética
Marcadores Genéticos
Apis mellifera
Varroa destructor
Argentina
Haplotypes
Heteroplasmy
Haplotipos
Heteroplasmia
title_short Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
title_full Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
title_fullStr Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
title_full_unstemmed Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
title_sort Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
dc.creator.none.fl_str_mv Muntaabski, Irina
Russo, Romina Maria
Liendo, María Clara
Palacio, María Alejandra
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author Muntaabski, Irina
author_facet Muntaabski, Irina
Russo, Romina Maria
Liendo, María Clara
Palacio, María Alejandra
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author_role author
author2 Russo, Romina Maria
Liendo, María Clara
Palacio, María Alejandra
Cladera, Jorge Luis
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genetic Variation
Genetic Markers
Variación Genética
Marcadores Genéticos
Apis mellifera
Varroa destructor
Argentina
Haplotypes
Heteroplasmy
Haplotipos
Heteroplasmia
topic Genetic Variation
Genetic Markers
Variación Genética
Marcadores Genéticos
Apis mellifera
Varroa destructor
Argentina
Haplotypes
Heteroplasmy
Haplotipos
Heteroplasmia
dc.description.none.fl_txt_mv Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor.
Instituto de Genética
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liendo, Marí­a Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-15T11:35:37Z
2020-09-15T11:35:37Z
2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7894
https://link.springer.com/article/10.1007/s00436-019-06591-5
1432-1955
https://doi.org/10.1007/s00436-019-06591-5
url http://hdl.handle.net/20.500.12123/7894
https://link.springer.com/article/10.1007/s00436-019-06591-5
https://doi.org/10.1007/s00436-019-06591-5
identifier_str_mv 1432-1955
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Parasitology Research 119 (2) : 411-421 (Febrero 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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