Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
- Autores
- Garcia, Julia Elena; Pagnussat, Luciana Anabella; Amenta, Melina B.; Casanovas, E. Mabel; Diaz, Pablo R.; Labarthe, María Mercedes; Martino, María V.; Groppa, María Daniela; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina
Fil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina - Fuente
- Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)
- Materia
-
Estrés Abiótico
Biofertilizantes
Maíz
Azospirillum
Trehalosa
Zea mays
Abiotic Stress
Biofertilizers
Maize
Trehalose
Azospirillum argentinense Az19
Inoculante
Inoculant - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21088
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Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulationGarcia, Julia ElenaPagnussat, Luciana AnabellaAmenta, Melina B.Casanovas, E. MabelDiaz, Pablo R.Labarthe, María MercedesMartino, María V.Groppa, María DanielaCreus, Cecilia MónicaMaroniche, Guillermo AndrésEstrés AbióticoBiofertilizantesMaízAzospirillumTrehalosaZea maysAbiotic StressBiofertilizersMaizeTrehaloseAzospirillum argentinense Az19InoculanteInoculantAzospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); ArgentinaFil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); ArgentinaFil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); ArgentinaFil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaSpringer2025-01-27T14:50:28Z2025-01-27T14:50:28Z2024-12-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21088https://link.springer.com/article/10.1007/s00253-024-13391-0Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)1432-0614 (Online)0175-7598https://doi.org/10.1007/s00253-024-13391-0Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestalesinfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:53Zoai:localhost:20.500.12123/21088instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:53.637INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
title |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
spellingShingle |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation Garcia, Julia Elena Estrés Abiótico Biofertilizantes Maíz Azospirillum Trehalosa Zea mays Abiotic Stress Biofertilizers Maize Trehalose Azospirillum argentinense Az19 Inoculante Inoculant |
title_short |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
title_full |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
title_fullStr |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
title_full_unstemmed |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
title_sort |
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
dc.creator.none.fl_str_mv |
Garcia, Julia Elena Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
author |
Garcia, Julia Elena |
author_facet |
Garcia, Julia Elena Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
author_role |
author |
author2 |
Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Estrés Abiótico Biofertilizantes Maíz Azospirillum Trehalosa Zea mays Abiotic Stress Biofertilizers Maize Trehalose Azospirillum argentinense Az19 Inoculante Inoculant |
topic |
Estrés Abiótico Biofertilizantes Maíz Azospirillum Trehalosa Zea mays Abiotic Stress Biofertilizers Maize Trehalose Azospirillum argentinense Az19 Inoculante Inoculant |
dc.description.none.fl_txt_mv |
Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina Fil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina |
description |
Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-12-27 2025-01-27T14:50:28Z 2025-01-27T14:50:28Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/21088 https://link.springer.com/article/10.1007/s00253-024-13391-0 Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024) 1432-0614 (Online) 0175-7598 https://doi.org/10.1007/s00253-024-13391-0 |
url |
http://hdl.handle.net/20.500.12123/21088 https://link.springer.com/article/10.1007/s00253-024-13391-0 https://doi.org/10.1007/s00253-024-13391-0 |
identifier_str_mv |
Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024) 1432-0614 (Online) 0175-7598 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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