Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation

Autores
Garcia, Julia Elena; Pagnussat, Luciana Anabella; Amenta, Melina B.; Casanovas, E. Mabel; Diaz, Pablo R.; Labarthe, María Mercedes; Martino, María V.; Groppa, María Daniela; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina
Fil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)
Materia
Estrés Abiótico
Biofertilizantes
Maíz
Azospirillum
Trehalosa
Zea mays
Abiotic Stress
Biofertilizers
Maize
Trehalose
Azospirillum argentinense Az19
Inoculante
Inoculant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21088
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21088
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulationGarcia, Julia ElenaPagnussat, Luciana AnabellaAmenta, Melina B.Casanovas, E. MabelDiaz, Pablo R.Labarthe, María MercedesMartino, María V.Groppa, María DanielaCreus, Cecilia MónicaMaroniche, Guillermo AndrésEstrés AbióticoBiofertilizantesMaízAzospirillumTrehalosaZea maysAbiotic StressBiofertilizersMaizeTrehaloseAzospirillum argentinense Az19InoculanteInoculantAzospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); ArgentinaFil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); ArgentinaFil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); ArgentinaFil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaSpringer2025-01-27T14:50:28Z2025-01-27T14:50:28Z2024-12-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21088https://link.springer.com/article/10.1007/s00253-024-13391-0Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)1432-0614 (Online)0175-7598https://doi.org/10.1007/s00253-024-13391-0Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestalesinfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:04Zoai:localhost:20.500.12123/21088instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:06.871INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
spellingShingle Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
Garcia, Julia Elena
Estrés Abiótico
Biofertilizantes
Maíz
Azospirillum
Trehalosa
Zea mays
Abiotic Stress
Biofertilizers
Maize
Trehalose
Azospirillum argentinense Az19
Inoculante
Inoculant
title_short Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_full Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_fullStr Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_full_unstemmed Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_sort Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
dc.creator.none.fl_str_mv Garcia, Julia Elena
Pagnussat, Luciana Anabella
Amenta, Melina B.
Casanovas, E. Mabel
Diaz, Pablo R.
Labarthe, María Mercedes
Martino, María V.
Groppa, María Daniela
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author Garcia, Julia Elena
author_facet Garcia, Julia Elena
Pagnussat, Luciana Anabella
Amenta, Melina B.
Casanovas, E. Mabel
Diaz, Pablo R.
Labarthe, María Mercedes
Martino, María V.
Groppa, María Daniela
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author_role author
author2 Pagnussat, Luciana Anabella
Amenta, Melina B.
Casanovas, E. Mabel
Diaz, Pablo R.
Labarthe, María Mercedes
Martino, María V.
Groppa, María Daniela
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrés Abiótico
Biofertilizantes
Maíz
Azospirillum
Trehalosa
Zea mays
Abiotic Stress
Biofertilizers
Maize
Trehalose
Azospirillum argentinense Az19
Inoculante
Inoculant
topic Estrés Abiótico
Biofertilizantes
Maíz
Azospirillum
Trehalosa
Zea mays
Abiotic Stress
Biofertilizers
Maize
Trehalose
Azospirillum argentinense Az19
Inoculante
Inoculant
dc.description.none.fl_txt_mv Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina
Fil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-27
2025-01-27T14:50:28Z
2025-01-27T14:50:28Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21088
https://link.springer.com/article/10.1007/s00253-024-13391-0
Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)
1432-0614 (Online)
0175-7598
https://doi.org/10.1007/s00253-024-13391-0
url http://hdl.handle.net/20.500.12123/21088
https://link.springer.com/article/10.1007/s00253-024-13391-0
https://doi.org/10.1007/s00253-024-13391-0
identifier_str_mv Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)
1432-0614 (Online)
0175-7598
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales
info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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