Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation

Autores
García, Julia E.; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Casanovas, Elda Mabel; Diaz, Pablo Rafael; Labarthe, María Mercedes; Martino, María V.; Groppa, María Daniela; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
Fil: García, Julia E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Casanovas, Elda Mabel. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Diaz, Pablo Rafael. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Martino, María V.. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Materia
PGPR
Abiotic stress
Osmolyre
Inoculant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/264924
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulationGarcía, Julia E.Pagnussat, Luciana AnabellaAmenta, Melina BeatrizCasanovas, Elda MabelDiaz, Pablo RafaelLabarthe, María MercedesMartino, María V.Groppa, María DanielaCreus, Cecilia MónicaMaroniche, Guillermo AndrésPGPRAbiotic stressOsmolyreInoculanthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.Fil: García, Julia E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Casanovas, Elda Mabel. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Diaz, Pablo Rafael. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Martino, María V.. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; ArgentinaSpringer2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264924García, Julia E.; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Casanovas, Elda Mabel; Diaz, Pablo Rafael; et al.; Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation; Springer; Applied Microbiology and Biotechnology; 108; 1; 12-20240175-7598CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s00253-024-13391-0info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-024-13391-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:19Zoai:ri.conicet.gov.ar:11336/264924instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:19.74CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
spellingShingle Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
García, Julia E.
PGPR
Abiotic stress
Osmolyre
Inoculant
title_short Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_full Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_fullStr Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_full_unstemmed Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
title_sort Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
dc.creator.none.fl_str_mv García, Julia E.
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Casanovas, Elda Mabel
Diaz, Pablo Rafael
Labarthe, María Mercedes
Martino, María V.
Groppa, María Daniela
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author García, Julia E.
author_facet García, Julia E.
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Casanovas, Elda Mabel
Diaz, Pablo Rafael
Labarthe, María Mercedes
Martino, María V.
Groppa, María Daniela
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author_role author
author2 Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Casanovas, Elda Mabel
Diaz, Pablo Rafael
Labarthe, María Mercedes
Martino, María V.
Groppa, María Daniela
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PGPR
Abiotic stress
Osmolyre
Inoculant
topic PGPR
Abiotic stress
Osmolyre
Inoculant
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
Fil: García, Julia E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Casanovas, Elda Mabel. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Diaz, Pablo Rafael. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Martino, María V.. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Introducción A Las Cs.agrarias. Area Biomolecular; Argentina
description Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/264924
García, Julia E.; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Casanovas, Elda Mabel; Diaz, Pablo Rafael; et al.; Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation; Springer; Applied Microbiology and Biotechnology; 108; 1; 12-2024
0175-7598
CONICET Digital
CONICET
url http://hdl.handle.net/11336/264924
identifier_str_mv García, Julia E.; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Casanovas, Elda Mabel; Diaz, Pablo Rafael; et al.; Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation; Springer; Applied Microbiology and Biotechnology; 108; 1; 12-2024
0175-7598
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s00253-024-13391-0
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-024-13391-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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