Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation

Autores
Pellegrini, María Celeste; Okada, Elena; Gonzalez Pasayo, Ramon Alejandro; Ponce, Alejandra Graciela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.
EEA Balcarce
Fil: Pellegrini, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: González Pasayo, Ramón Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Ponce, Alejandra Graciela. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Comisión de Investigaciones Científicas; Argentina. Instituto de Ciencia y Tecnología de los alimentos; Argentina.
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fuente
Environmental Science and Pollution Research 29 : 23225-23236 (2022)
Materia
Escherichia Coli
Suelo
Aguas Subterráneas
Cama (animales)
Antibióticos
Biofilmes (microbiología)
Soil
Groundwater
Litter for Animals
Antibiotics
Biofilms (microbiology)
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13375
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13375
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliationPellegrini, María CelesteOkada, ElenaGonzalez Pasayo, Ramon AlejandroPonce, Alejandra GracielaEscherichia ColiSueloAguas SubterráneasCama (animales)AntibióticosBiofilmes (microbiología)SoilGroundwaterLitter for AnimalsAntibioticsBiofilms (microbiology)Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.EEA BalcarceFil: Pellegrini, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: González Pasayo, Ramón Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Ponce, Alejandra Graciela. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Comisión de Investigaciones Científicas; Argentina. Instituto de Ciencia y Tecnología de los alimentos; Argentina.Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Springer2022-11-10T14:21:40Z2022-11-10T14:21:40Z2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13375https://link.springer.com/article/10.1007/s11356-021-17523-11614-7499https://doi.org/10.1007/s11356-021-17523-1Environmental Science and Pollution Research 29 : 23225-23236 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E2-I518-002/2019-PD-E2-I518-002/AR./Estudio del impacto ambiental, gestión y tratamiento de residuos y efluentes sobre sistemas agropecuarios y agroindustriales para su valorización agronómicainfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:59Zoai:localhost:20.500.12123/13375instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:59.472INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
title Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
spellingShingle Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
Pellegrini, María Celeste
Escherichia Coli
Suelo
Aguas Subterráneas
Cama (animales)
Antibióticos
Biofilmes (microbiología)
Soil
Groundwater
Litter for Animals
Antibiotics
Biofilms (microbiology)
title_short Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
title_full Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
title_fullStr Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
title_full_unstemmed Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
title_sort Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
dc.creator.none.fl_str_mv Pellegrini, María Celeste
Okada, Elena
Gonzalez Pasayo, Ramon Alejandro
Ponce, Alejandra Graciela
author Pellegrini, María Celeste
author_facet Pellegrini, María Celeste
Okada, Elena
Gonzalez Pasayo, Ramon Alejandro
Ponce, Alejandra Graciela
author_role author
author2 Okada, Elena
Gonzalez Pasayo, Ramon Alejandro
Ponce, Alejandra Graciela
author2_role author
author
author
dc.subject.none.fl_str_mv Escherichia Coli
Suelo
Aguas Subterráneas
Cama (animales)
Antibióticos
Biofilmes (microbiología)
Soil
Groundwater
Litter for Animals
Antibiotics
Biofilms (microbiology)
topic Escherichia Coli
Suelo
Aguas Subterráneas
Cama (animales)
Antibióticos
Biofilmes (microbiología)
Soil
Groundwater
Litter for Animals
Antibiotics
Biofilms (microbiology)
dc.description.none.fl_txt_mv Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.
EEA Balcarce
Fil: Pellegrini, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: González Pasayo, Ramón Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Ponce, Alejandra Graciela. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Comisión de Investigaciones Científicas; Argentina. Instituto de Ciencia y Tecnología de los alimentos; Argentina.
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
description Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-10T14:21:40Z
2022-11-10T14:21:40Z
2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13375
https://link.springer.com/article/10.1007/s11356-021-17523-1
1614-7499
https://doi.org/10.1007/s11356-021-17523-1
url http://hdl.handle.net/20.500.12123/13375
https://link.springer.com/article/10.1007/s11356-021-17523-1
https://doi.org/10.1007/s11356-021-17523-1
identifier_str_mv 1614-7499
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E2-I518-002/2019-PD-E2-I518-002/AR./Estudio del impacto ambiental, gestión y tratamiento de residuos y efluentes sobre sistemas agropecuarios y agroindustriales para su valorización agronómica
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Environmental Science and Pollution Research 29 : 23225-23236 (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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